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[184135_s_at]

Description F49E8.1 /REP_DB=WormBase Gene ID /WP=CE28406 /TR=Q20633 /GB=AAB03156.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 56      6grp_high1_10_10 > 65      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_highest_nonorm > 82      6grp_total > 55      4grp_total > 60      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 209
1 2 3 4 range
A 371.6
(3)
P 		356.3
(2.4)
P 		320.9
(1.2)
P 		206.3
(-3)
P 		165
B 364.4
(2.3)
P 		327
(1.1)
P 		386.4
(3)
P 		199.6
(-3)
P 		187
C 404.4
(2.9)
P 		405.7
(2.9)
P 		408.9
(3)
P 		206.3
(-3)
P 		203
avr 380.1
(3) 		363
(2.4) 		372.1
(2.7) 		204.1
(-3) 		176

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 137
1 2 3 4 5 6 range
A 390.8
(2.4)
P 		330.8
(-0.8)
P 		402.4
(3)
P 		290.5
(-3)
P 		310.6
(-1.9)
P 		389.2
(2.3)
P 		112
B 348.7
(-1.3)
P 		356.8
(-0.9)
P 		427.7
(3)
P 		390.1
(0.9)
P 		318.1
(-3)
P 		412.9
(2.2)
P 		110
C 302.3
(-3)
P 		364.6
(0.3)
P 		352.3
(-0.4)
P 		337.9
(-1.1)
P 		355.6
(-0.2)
P 		416.9
(3)
P 		115
avr 347.3
(-1.5) 		350.7
(-1.3) 		394.1
(2.1) 		339.5
(-2.1) 		328.1
(-3) 		406.3
(3) 		78
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 135.3
(3)
P 		77.3
(-0.3)
P 		83.9
(0.1)
P 		30.2
(-3)
P 		105
B 116.2
(3)
P 		80.8
(0.1)
P 		89.5
(0.8)
P 		42.5
(-3)
P 		74
C 133.9
(2.5)
P 		116.6
(1.6)
P 		142.5
(3)
P 		34.8
(-3)
P 		108
avr 128.5
(3) 		91.6
(0.6) 		105.3
(1.5) 		35.8
(-3) 		93

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 72
(-3)
P 		117.7
(3)
P 		87.6
(-1)
P 		73.7
(-2.8)
P 		93.7
(-0.2)
P 		94.7
(-0)
P 		46
B 121.3
(-0.9)
P 		118.8
(-1.2)
P 		103.9
(-2.9)
P 		103.3
(-3)
P 		129.2
(0.1)
P 		154
(3)
P 		51
C 112.1
(0.7)
P 		111.2
(0.6)
P 		89.7
(-1.2)
P 		67.9
(-3)
P 		115.1
(1)
P 		139.5
(3)
P 		72
avr 101.8
(-0.5) 		115.9
(1.3) 		93.7
(-1.5) 		81.6
(-3) 		112.7
(0.9) 		129.4
(3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184135_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10104
Sequence Type Exemplar sequence
Representative Public ID F49E8.1 NCBI
Target Description F49E8.1 /REP_DB=WormBase Gene ID /WP=CE28406 /TR=Q20633 /GB=AAB03156.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068825(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068825 NCBI Caenorhabditis elegans F49E8.1 (F49E8.1) mRNA, complete cds. 11/11 None
F49E8.1 ENSEMBL cdna:known chromosome:CEL140:IV:7556652:7558733:1 gene:F49E8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7556638-7558686(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49E8.1
Entrez Gene 177532 Entrez gene
SwissProt Q20633 EMBL-EBI
WormBase CE33650 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1584669_AT similar to Tumor suppressor candidate 4 (NPR2-like) (Gene 21 protein) (G21 protein) cfa
CANINE_2:CFA.19822.1.S1_S_AT similar to Tumor suppressor candidate 4 (NPR2-like) (Gene 21 protein) (G21 protein) cfa
CANINE_2:CFA.6418.1.A1_AT similar to Tumor suppressor candidate 4 (NPR2-like) (Gene 21 protein) (G21 protein) cfa
CANINE_2:CFAAFFX.16554.1.S1_AT similar to Tumor suppressor candidate 4 (NPR2-like) (Gene 21 protein) (G21 protein) cfa
DROSOPHILA_2:1640290_AT dm
DROSGENOME1:154897_AT dm
HG-U133_PLUS_2:210373_AT tumor suppressor candidate 4 hs
HG-U133A:210373_AT tumor suppressor candidate 4 hs
HG-U133A_2:210373_AT tumor suppressor candidate 4 hs
HG-U133_PLUS_2:203246_S_AT tumor suppressor candidate 4 hs
HG-U133A:203246_S_AT tumor suppressor candidate 4 hs
HG-U133A_2:203246_S_AT tumor suppressor candidate 4 hs
HG-U95AV2:40499_R_AT tumor suppressor candidate 4 hs
HU35KSUBA:RC_AA599751_AT tumor suppressor candidate 4 hs
U133_X3P:G2935584_3P_A_AT tumor suppressor candidate 4 hs
HG-U95AV2:40497_AT tumor suppressor candidate 4 hs
HG-U95AV2:40498_G_AT tumor suppressor candidate 4 hs
MU11KSUBB:MSA.13871.0_S_AT tumor suppressor candidate 4 mm
MG-U74BV2:162550_F_AT tumor suppressor candidate 4 mm
MOE430A:1417046_AT tumor suppressor candidate 4 mm
MOUSE430A_2:1417046_AT tumor suppressor candidate 4 mm
MOUSE430_2:1417046_AT tumor suppressor candidate 4 mm
MU19KSUBA:TC14567_AT Tumor suppressor candidate 4, mRNA (cDNA clone MGC:35941 IMAGE:5028027) mm
RG-U34C:RC_AI170249_AT tumor suppressor candidate 4 (predicted) rn
RAE230A:1375858_AT tumor suppressor candidate 4 (predicted) rn
RAT230_2:1375858_AT tumor suppressor candidate 4 (predicted) rn
RAE230B:1394580_AT tumor suppressor candidate 4 (predicted) rn
RAT230_2:1394580_AT tumor suppressor candidate 4 (predicted) rn
YEAST_2:1770211_AT Regulator of nitrogen permeases; transcription is induced in response to proline and urea; contains two PEST sequences Sc
Protein Similarities
Method ID Description E-Value
blast AAB03156 Hypothetical protein F49E8.1 [Caenorhabditis elegans] ref|NP_501226.2| F49E8.1 [Caenorhabditis elegans] 0.0
blast CAE61915 Hypothetical protein CBG05911 [Caenorhabditis briggsae] 0.0
blast XP_392834 PREDICTED: similar to CG9104-PA [Apis mellifera] 2.0E-44
Protein Domains
Database ID Description E-Value
Pfam IPR009348 EMBL-EBI Nitrogen permease regulator 2 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:184135_S_AT
agaaattccaacgcgaactccagaattttcggatgtgttccgactttatttgagcatgaa
agtggtgggacctaaacttccgatcgacgaatctgatagcaatcgactacgttttcagtg
cggcaacaatctagcagaatgggccgatctggagaaaccaagatcgatgggtattgatga
acagcgacttgtacaatttggaatgcatcatcagtttcttcgtaaactttctaaatatcc
tatttgtgttgcaaacccaggcaacaacactctcctgaaacggtgtaatggaaaaacttc
aattgacgaactttctcttcaacataatgtggaccccgaacaactttgctcactgttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAATTCCAACGCGAACTCCAGAA 509 71 1335 Antisense
ACTCCAGAATTTTCGGATGTGTTCC 27 99 1351 Antisense
GATGTGTTCCGACTTTATTTGAGCA 127 409 1366 Antisense
ACAATCTAGCAGAATGGGCCGATCT 183 115 1460 Antisense
GATGGGTATTGATGAACAGCGACTT 209 407 1500 Antisense
ATTTGGAATGCATCATCAGTTTCTT 154 19 1530 Antisense
TCCTATTTGTGTTGCAAACCCAGGC 397 583 1572 Antisense
GCAACAACACTCTCCTGAAACGGTG 357 317 1595 Antisense
GACGAACTTTCTCTTCAACATAATG 165 379 1639 Antisense
AACATAATGTGGACCCCGAACAACT 327 129 1655 Antisense
CCCCGAACAACTTTGCTCACTGTTG 496 261 1668 Antisense
Sequence Source Affymetrix Proprietary Database