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[184134_at]

Description F49E8.1 /REP_DB=WormBase Gene ID /WP=CE28406 /TR=Q20633 /GB=AAB03156.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 74      6grp_high1_10_10 > 47      4grp_highest_8_8 > 41      4grp_high_8_8 > 50      6grp_highest_nonorm > 81      6grp_total > 47      4grp_total > 62      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 322.2
(3)
P 		225.2
(-0.2)
P 		215.8
(-0.5)
P 		138
(-3)
P 		184
B 307.3
(3)
P 		241.1
(0.6)
P 		267.5
(1.6)
P 		140.5
(-3)
P 		167
C 298.6
(3)
P 		241.2
(0.5)
P 		292.2
(2.7)
P 		163
(-3)
P 		136
avr 309.4
(3) 		235.8
(0.3) 		258.5
(1.1) 		147.2
(-3) 		162

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 150
1 2 3 4 5 6 range
A 318
(3)
P 		294.7
(1.9)
P 		255.5
(0.1)
P 		249.1
(-0.2)
P 		189.5
(-3)
P 		262.3
(0.4)
P 		129
B 312.3
(1.4)
P 		274.6
(-0.9)
P 		282.7
(-0.4)
P 		238.5
(-3)
P 		260.9
(-1.7)
P 		339.8
(3)
P 		101
C 259.8
(-0.1)
P 		290.6
(1.4)
P 		243.2
(-1)
P 		202.1
(-3)
P 		222.6
(-2)
P 		322.7
(3)
P 		121
avr 296.7
(2.2) 		286.6
(1.5) 		260.5
(-0.4) 		229.9
(-2.6) 		224.3
(-3) 		308.3
(3) 		84
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 117.3
(3)
P 		48.9
(-1.2)
P 		56.4
(-0.8)
P 		20.2
(-3)
P 		97
B 98
(3)
P 		59.5
(-0.4)
P 		62
(-0.2)
P 		29.9
(-3)
P 		68
C 98.9
(2.8)
P 		69.3
(0.4)
P 		101.8
(3)
P 		27.5
(-3)
P 		74
avr 104.7
(3) 		59.2
(-0.5) 		73.4
(0.6) 		25.9
(-3) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 58.6
(-2.6)
P 		104.8
(3)
P 		55.7
(-3)
P 		63.2
(-2.1)
P 		57.2
(-2.8)
P 		63.9
(-2)
P 		49
B 108.7
(1.3)
P 		91.4
(-0.3)
P 		68.7
(-2.5)
P 		63.2
(-3)
P 		106
(1)
P 		126.7
(3)
P 		64
C 96.3
(2)
P 		88.7
(1.3)
P 		61.9
(-1.1)
P 		40.6
(-3)
P 		72
(-0.2)
P 		108
(3)
P 		67
avr 87.9
(1.4) 		95
(2.4) 		62.1
(-2.1) 		55.7
(-3) 		78.4
(0.1) 		99.5
(3) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184134_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10104
Sequence Type Exemplar sequence
Representative Public ID F49E8.1 NCBI
Target Description F49E8.1 /REP_DB=WormBase Gene ID /WP=CE28406 /TR=Q20633 /GB=AAB03156.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068825
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068825 NCBI Caenorhabditis elegans F49E8.1 (F49E8.1) mRNA, complete cds. A 
F49E8.1 ENSEMBL cdna:known chromosome:CEL140:IV:7556652:7558733:1 gene:F49E8.1 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7556638-7558686(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49E8.1
Entrez Gene 177532 Entrez gene
SwissProt Q20633 EMBL-EBI
WormBase CE33650 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1584669_AT similar to Tumor suppressor candidate 4 (NPR2-like) (Gene 21 protein) (G21 protein) cfa
CANINE_2:CFA.19822.1.S1_S_AT similar to Tumor suppressor candidate 4 (NPR2-like) (Gene 21 protein) (G21 protein) cfa
CANINE_2:CFA.6418.1.A1_AT similar to Tumor suppressor candidate 4 (NPR2-like) (Gene 21 protein) (G21 protein) cfa
CANINE_2:CFAAFFX.16554.1.S1_AT similar to Tumor suppressor candidate 4 (NPR2-like) (Gene 21 protein) (G21 protein) cfa
DROSOPHILA_2:1640290_AT dm
DROSGENOME1:154897_AT dm
HG-U133_PLUS_2:210373_AT tumor suppressor candidate 4 hs
HG-U133A:210373_AT tumor suppressor candidate 4 hs
HG-U133A_2:210373_AT tumor suppressor candidate 4 hs
HG-U133_PLUS_2:203246_S_AT tumor suppressor candidate 4 hs
HG-U133A:203246_S_AT tumor suppressor candidate 4 hs
HG-U133A_2:203246_S_AT tumor suppressor candidate 4 hs
HG-U95AV2:40499_R_AT tumor suppressor candidate 4 hs
HU35KSUBA:RC_AA599751_AT tumor suppressor candidate 4 hs
U133_X3P:G2935584_3P_A_AT tumor suppressor candidate 4 hs
HG-U95AV2:40497_AT tumor suppressor candidate 4 hs
HG-U95AV2:40498_G_AT tumor suppressor candidate 4 hs
MU11KSUBB:MSA.13871.0_S_AT tumor suppressor candidate 4 mm
MG-U74BV2:162550_F_AT tumor suppressor candidate 4 mm
MOE430A:1417046_AT tumor suppressor candidate 4 mm
MOUSE430A_2:1417046_AT tumor suppressor candidate 4 mm
MOUSE430_2:1417046_AT tumor suppressor candidate 4 mm
MU19KSUBA:TC14567_AT Tumor suppressor candidate 4, mRNA (cDNA clone MGC:35941 IMAGE:5028027) mm
RG-U34C:RC_AI170249_AT tumor suppressor candidate 4 (predicted) rn
RAE230A:1375858_AT tumor suppressor candidate 4 (predicted) rn
RAT230_2:1375858_AT tumor suppressor candidate 4 (predicted) rn
RAE230B:1394580_AT tumor suppressor candidate 4 (predicted) rn
RAT230_2:1394580_AT tumor suppressor candidate 4 (predicted) rn
YEAST_2:1770211_AT Regulator of nitrogen permeases; transcription is induced in response to proline and urea; contains two PEST sequences Sc
Protein Similarities
Method ID Description E-Value
blast AAB03156 Hypothetical protein F49E8.1 [Caenorhabditis elegans] ref|NP_501226.2| F49E8.1 [Caenorhabditis elegans] 0.0
blast CAE61915 Hypothetical protein CBG05911 [Caenorhabditis briggsae] 0.0
blast XP_392834 PREDICTED: similar to CG9104-PA [Apis mellifera] 2.0E-44
Protein Domains
Database ID Description E-Value
Pfam IPR009348 EMBL-EBI Nitrogen permease regulator 2 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:184134_AT
acagttcgagagattgctattattctcgaacttgatccatccctcgtcgccagatgtgtt
cgtaatttacacttctacgaatgtgtcagtcttgttccaatgtttctctactataataca
tatgtggcaactgaaagagttcacgacttttacaaaaatcaaacggaaatcaatgactgc
cttgagtttgtgaaaattcaaacggaaatcggtgcagacggctcagaaattccaacgcga
actccagaattttcggatgtgttccgactttatttgagcatgaaagtggtgggacctaaa
cttccgatcgacgaatctgatagcaatcgactacgttttcagtgcggcaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAGTTCGAGAGATTGCTATTATTC 174 107 1111 Antisense
ATTATTCTCGAACTTGATCCATCCC 635 13 1129 Antisense
CCTCGTCGCCAGATGTGTTCGTAAT 692 265 1152 Antisense
CTACGAATGTGTCAGTCTTGTTCCA 246 225 1185 Antisense
GTCTTGTTCCAATGTTTCTCTACTA 188 469 1199 Antisense
CAATGACTGCCTTGAGTTTGTGAAA 269 183 1281 Antisense
GGAAATCGGTGCAGACGGCTCAGAA 286 527 1314 Antisense
TGGTGGGACCTAAACTTCCGATCGA 446 551 1397 Antisense
AAACTTCCGATCGACGAATCTGATA 536 127 1408 Antisense
GAATCTGATAGCAATCGACTACGTT 500 329 1423 Antisense
TCGACTACGTTTTCAGTGCGGCAAC 437 599 1437 Antisense
Sequence Source Affymetrix Proprietary Database