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[184127_at] guanine-nucleotide releasing factor

Description F07C3.5 /REP_DB=WormBase Gene ID /WP=CE07027 /TR=Q19150 /GB=AAG24029.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 10      4grp_total > 12      6grp_cgc > 55      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 134
1 2 3 4 range
A 51.7
(-0.6)
A 		19.7
(-2.5)
A 		11
(-3)
A 		113
(3)
A 		102
B 72.7
(3)
A 		13.6
(-3)
A 		18.8
(-2.5)
A 		39.7
(-0.4)
A 		59
C 11.8
(-2.8)
A 		31.9
(-1.9)
A 		6.9
(-3)
A 		141.2
(3)
A 		134
avr 45.4
(-0.7) 		21.7
(-2.3) 		12.2
(-3) 		98
(3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 12.2
(-3)
A 		24.8
(3)
A 		22.6
(2)
A 		20
(0.7)
A 		16.5
(-1)
A 		21.2
(1.3)
A 		13
B 10.6
(-0.2)
A 		12.9
(1.7)
A 		14.5
(3)
A 		13.4
(2.1)
A 		11.2
(0.3)
A 		7.2
(-3)
A 		7
C 15.2
(1.4)
A 		12.7
(-0.3)
A 		9.7
(-2.4)
A 		15.2
(1.4)
A 		8.9
(-3)
A 		17.5
(3)
A 		9
avr 12.7
(-2.3) 		16.8
(3) 		15.6
(1.4) 		16.2
(2.2) 		12.2
(-3) 		15.3
(1) 		5
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 18.8
(3)
A 		4.3
(-2.5)
A 		2.9
(-3)
A 		16.5
(2.1)
A 		16
B 23.2
(3)
A 		3.4
(-3)
A 		4.4
(-2.7)
A 		8.5
(-1.5)
A 		20
C 3.9
(-2.6)
A 		9.2
(-1.1)
A 		2.4
(-3)
A 		23.8
(3)
A 		21
avr 15.3
(2.5) 		5.6
(-1.9) 		3.2
(-3) 		16.3
(3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 2.2
(-3)
A 		8.8
(3)
A 		4.9
(-0.5)
A 		5.1
(-0.4)
A 		5
(-0.5)
A 		5.2
(-0.3)
A 		7
B 3.7
(0.3)
A 		4.3
(2.3)
A 		3.5
(-0.3)
A 		3.6
(0)
A 		4.5
(3)
A 		2.7
(-3)
A 		2
C 5.6
(2.5)
A 		3.9
(-0.5)
A 		2.5
(-3)
A 		3.1
(-1.9)
A 		2.9
(-2.3)
A 		5.9
(3)
A 		3
avr 3.8
(-2.4) 		5.7
(3) 		3.6
(-3) 		3.9
(-2.1) 		4.1
(-1.6) 		4.6
(-0.1) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184127_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12078
Sequence Type Exemplar sequence
Representative Public ID F07C3.5 NCBI
Target Description F07C3.5 /REP_DB=WormBase Gene ID /WP=CE07027 /TR=Q19150 /GB=AAG24029.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072991(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072991 NCBI Caenorhabditis elegans Gut granule LOss family member (glo-4) (glo-4) mRNA, complete cds. 11/11 None
SNAP00000009706 ENSEMBL cdna:SNAP chromosome:CEL140:V:9242705:9245749:-1 11/11 None
GENEFINDER00000009730 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9237571:9245749:-1 11/11 None
F07C3.4 ENSEMBL cdna:known chromosome:CEL140:V:9237209:9245749:-1 gene:F07C3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9243478-9245749(-) 100.0 100.0
Public Domain and Genome References
Gene Title guanine-nucleotide releasing factor
Gene Symbol glo-4
Ensembl F07C3.4
Entrez Gene 179306 Entrez gene
SwissProt Q5DX34 EMBL-EBI
WormBase CE38062 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_505392 Gut granule LOss family member (glo-4) [Caenorhabditis elegans] gb|AAW88404.1| Hypothetical protein F07C3.4 [Caenorhabditis elegans] sp|Q5DX34|RPGRH_CAEEL X-linked retinitis pigmentosa GTPase regulator homolog 0.0
blast CAE72137 Hypothetical protein CBG19235 [Caenorhabditis briggsae] 0.0
blast CAE72138 Hypothetical protein CBG19236 [Caenorhabditis briggsae] 1.0E-123
Protein Domains
Database ID Description E-Value
Pfam IPR000408 EMBL-EBI Regulator of chromosome condensation, RCC1 2.9E-7
Pfam IPR000408 EMBL-EBI Regulator of chromosome condensation, RCC1 2.9E-7
Sequence
Target Sequence 
>C. ELEGANS:184127_AT
gattcgtttcttctaatcttgctgcttacgtcaaaaggagacgttcaagcttatttggag
acaagtcgagacacaaagccaagaaaaaacgttgtaaatatcaaaacggcatgctccctc
aatgttagcccggttactgtatgcattggaagtcaagcagcattcgccatctttggactt
gctgatggtaatcttctcgttactccaatcaggcttcttatcgacgtgacgtggggtggt
tcctcgtgggcgacaacgactgtaattgatttgagtcttccaacagtggatccttgcctg
gccacacccacttgcactaaatgcttcgtttccaattttccaccgtgcactatggctgtg
gttgcaaataaagctgggaacattcttctcgtcgatcttcacctcagaaaatgtgtgtcc
gagttgaaagcaccgcaaagtttgcaccagattgacattcttcttgatgaaaactctatc
gagttgcttgtcacgggatttactggtgcacaatggataattccaattgaaagaagtgga
aagggatttcgagaagttttgacaacatgcgtaccatcagatcttactg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCGTTTCTTCTAATCTTGCTGC 28 433 271 Antisense
AATATCAAAACGGCATGCTCCCTCA 255 173 367 Antisense
CCCTCAATGTTAGCCCGGTTACTGT 109 1 386 Antisense
TCAAGCAGCATTCGCCATCTTTGGA 575 631 423 Antisense
TACTCCAATCAGGCTTCTTATCGAC 420 645 471 Antisense
GTCTTCCAACAGTGGATCCTTGCCT 226 467 545 Antisense
CCACACCCACTTGCACTAAATGCTT 655 273 572 Antisense
CCAATTTTCCACCGTGCACTATGGC 595 277 602 Antisense
AAAGCTGGGAACATTCTTCTCGTCG 394 121 640 Antisense
CTCTATCGAGTTGCTTGTCACGGGA 498 227 744 Antisense
ACATGCGTACCATCAGATCTTACTG 393 105 835 Antisense
Sequence Source Affymetrix Proprietary Database