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[184114_at]

Description F25E5.7 /REP_DB=WormBase Gene ID /WP=CE07141 /TR=O76649 /GB=AAG24078.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 66      4grp_total > 01      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 244
1 2 3 4 range
A 6.2
(-3)
A 		9.4
(-2.9)
A 		49.5
(-1.9)
A 		250.5
(3)
P 		244
B 22.5
(-2.6)
A 		9
(-3)
A 		27.8
(-2.4)
A 		203.1
(3)
P 		194
C 34
(-1.9)
A 		8.6
(-2.9)
A 		6.8
(-3)
A 		160.7
(3)
P 		154
avr 20.9
(-2.6) 		9
(-3) 		28
(-2.4) 		204.8
(3) 		196

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 6.9
(-2.7)
A 		26
(-0.7)
A 		26.7
(-0.6)
A 		61
(3)
A 		4.4
(-3)
A 		51.9
(2)
A 		57
B 19.8
(-1.9)
A 		28.4
(-1.2)
A 		28.2
(-1.2)
A 		5.9
(-3)
A 		32.7
(-0.8)
A 		80.3
(3)
A 		74
C 8.7
(-2.5)
A 		8.9
(-2.5)
A 		3
(-3)
A 		12.6
(-2.2)
A 		7.6
(-2.6)
A 		78.7
(3)
P 		76
avr 11.8
(-3) 		21.1
(-2) 		19.3
(-2.2) 		26.5
(-1.5) 		14.9
(-2.7) 		70.3
(3) 		59
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 2.3
(-2.9)
A 		2
(-3)
A 		12.9
(-1.1)
A 		36.7
(3)
P 		35
B 7.2
(-2.3)
A 		2.2
(-3)
A 		6.5
(-2.4)
A 		43.3
(3)
P 		41
C 11.3
(-0.8)
A 		2.5
(-3)
A 		2.4
(-3)
A 		27.1
(3)
P 		25
avr 6.9
(-2.2) 		2.2
(-3) 		7.3
(-2.1) 		35.7
(3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 1.3
(-3)
A 		9.3
(0.4)
A 		5.8
(-1.1)
A 		15.5
(3)
A 		1.3
(-3)
A 		12.6
(1.8)
A 		14
B 6.9
(-1.9)
A 		9.5
(-1.3)
A 		6.8
(-1.9)
A 		1.6
(-3)
A 		13.3
(-0.5)
A 		29.9
(3)
A 		28
C 3.2
(-2.4)
A 		2.7
(-2.6)
A 		0.8
(-3)
A 		2.5
(-2.6)
A 		2.5
(-2.6)
A 		26.3
(3)
P 		26
avr 3.8
(-3) 		7.2
(-1.9) 		4.5
(-2.8) 		6.5
(-2.2) 		5.7
(-2.4) 		22.9
(3) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184114_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11361
Sequence Type Exemplar sequence
Representative Public ID F25E5.7 NCBI
Target Description F25E5.7 /REP_DB=WormBase Gene ID /WP=CE07141 /TR=O76649 /GB=AAG24078.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182435(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182435 NCBI Caenorhabditis elegans F25E5.7 (F25E5.7) mRNA, complete cds. 11/11 None
SNAP00000010676 ENSEMBL cdna:SNAP chromosome:CEL140:V:7442712:7449304:1 11/11 None
GENEFINDER00000010689 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7442712:7449304:1 11/11 None
F25E5.7 ENSEMBL cdna:known chromosome:CEL140:V:7442712:7444075:1 gene:F25E5.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7440914-7444076(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25E5.7
Entrez Gene 353443 Entrez gene
SwissProt O76649 EMBL-EBI
WormBase CE32875 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN84817 Hypothetical protein F25E5.7 [Caenorhabditis elegans] ref|NP_872235.1| F25E5.7 [Caenorhabditis elegans] 0.0
blast F89101 protein F25E5.7 [imported] - Caenorhabditis elegans 0.0
blast AAQ62458 Hypothetical protein F25E5.6b [Caenorhabditis elegans] ref|NP_001023845.1| F25E5.6b [Caenorhabditis elegans] 0.0
blast G89101 protein F25E5.6 [imported] - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 3.6E-49
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 9.4E-21
Sequence
Target Sequence 
>C. ELEGANS:184114_AT
gaagccactggagttgaacccttcaatttggccagtatgcatttcgaacgatccacaatt
atttgacagatcaagtgatttctctgtgtctggtatagacgctaagggtgttttgaattc
gggattgttcaagccagttaattgctcagttgaaggacctttttcatgtgccgaagcggt
agataataaacaacgaatgtgcgcatatgactccggaggtagtgcaataagcaacgtttc
cggacaaaataccgttcttggagtgtatgtaacagggaacatgaactgcaatgcgaatcc
acagaacctcacagacttcaaattcataaacattgctaaccatcgagagtcgatttgcaa
gctaacaggaatatgtgtgccagatccagagagaatcgatcccacatcatccgcacttcc
accaactccatacgaaccagttggttatgacggagaagttttaaccgctgaacacttcga
ccctatgagacttaatggttcggaccccgcggaatccatcagagcggagagaattgaccc
gatgaaactgaacggttcagcgcatcatggag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCCACTGGAGTTGAACCCTTCA 309 345 1410 Antisense
GAACCCTTCAATTTGGCCAGTATGC 195 347 1425 Antisense
ACCTTTTTCATGTGCCGAAGCGGTA 467 83 1566 Antisense
GAATGTGCGCATATGACTCCGGAGG 257 331 1604 Antisense
TGCAATGCGAATCCACAGAACCTCA 50 577 1696 Antisense
AACATTGCTAACCATCGAGAGTCGA 431 137 1738 Antisense
AGAGAGAATCGATCCCACATCATCC 598 67 1797 Antisense
AACACTTCGACCCTATGAGACTTAA 401 135 1880 Antisense
TGAGACTTAATGGTTCGGACCCCGC 212 567 1895 Antisense
GACCCCGCGGAATCCATCAGAGCGG 577 381 1912 Antisense
CTGAACGGTTCAGCGCATCATGGAG 660 235 1957 Antisense
Sequence Source Affymetrix Proprietary Database