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[184112_at]

Description W06B4.3 /REP_DB=WormBase Gene ID /WP=CE25141 /TR=Q23194 /GB=AAC46819.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      4grp_high_8_8 > 40      6grp_highest_nonorm > 81      6grp_total > 26      4grp_total > 54      6grp_highest_sub > 01_02      6grp_high1_sub > 01_02      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 250
1 2 3 4 range
A 279.8
(3)
P 		176
(-1.9)
P 		153.9
(-3)
P 		157.1
(-2.8)
P 		126
B 329.6
(3)
P 		218.3
(0.3)
P 		219.9
(0.4)
P 		79.9
(-3)
A 		250
C 294.6
(3)
P 		169.3
(-1.6)
P 		210.8
(-0.1)
P 		131.1
(-3)
A 		164
avr 301.3
(3) 		187.9
(-0.8) 		194.9
(-0.6) 		122.7
(-3) 		179

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 137
1 2 3 4 5 6 range
A 360.6
(3)
P 		319.3
(0.9)
P 		243.2
(-2.8)
P 		240
(-3)
P 		297.6
(-0.1)
P 		298.8
(-0.1)
P 		121
B 359.2
(3)
P 		357.4
(2.9)
P 		295.3
(-1.8)
P 		334.4
(1.1)
P 		279.6
(-3)
P 		285.3
(-2.6)
P 		80
C 283.5
(0.1)
P 		338.5
(3)
P 		223.7
(-3)
P 		300.8
(1)
P 		230.1
(-2.7)
P 		291.9
(0.6)
P 		115
avr 334.4
(2.7) 		338.4
(3) 		254.1
(-3) 		291.7
(-0.3) 		269.1
(-1.9) 		292
(-0.3) 		84
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 101.9
(3)
P 		38.2
(-1.8)
P 		40.2
(-1.7)
P 		23
(-3)
P 		79
B 105.1
(3)
P 		53.9
(-0.5)
P 		50.9
(-0.7)
P 		17
(-3)
A 		88
C 97.5
(3)
P 		48.7
(-0.9)
P 		73.5
(1.1)
P 		22.1
(-3)
A 		75
avr 101.5
(3) 		46.9
(-1.1) 		54.9
(-0.5) 		20.7
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 66.5
(-1.7)
P 		113.5
(3)
P 		53
(-3)
P 		60.9
(-2.2)
P 		89.8
(0.6)
P 		72.7
(-1)
P 		61
B 125
(3)
P 		119
(2.3)
P 		71.7
(-3)
P 		88.6
(-1.1)
P 		113.6
(1.7)
P 		106.4
(0.9)
P 		53
C 105.1
(3)
P 		103.3
(2.8)
P 		56.9
(-3)
P 		60.5
(-2.6)
P 		74.5
(-0.8)
P 		97.7
(2.1)
P 		48
avr 98.9
(1.5) 		111.9
(3) 		60.5
(-3) 		70
(-1.9) 		92.6
(0.7) 		92.3
(0.7) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184112_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5069
Sequence Type Exemplar sequence
Representative Public ID W06B4.3 NCBI
Target Description W06B4.3 /REP_DB=WormBase Gene ID /WP=CE25141 /TR=Q23194 /GB=AAC46819.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062387(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062387 NCBI Caenorhabditis elegans W06B4.3 (W06B4.3) mRNA, complete cds. 11/11 None
SNAP00000036999 ENSEMBL cdna:SNAP chromosome:CEL140:II:4461418:4467721:1 11/11 None
GENEFINDER00000037004 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4461420:4467721:1 11/11 None
W06B4.3 ENSEMBL cdna:known chromosome:CEL140:II:4461420:4467854:1 gene:W06B4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4461418-4467720(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W06B4.3
Entrez Gene 173783 Entrez gene
SwissProt Q23194 EMBL-EBI
WormBase CE32071 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC46819 Hypothetical protein W06B4.3 [Caenorhabditis elegans] ref|NP_494788.2| W06B4.3 [Caenorhabditis elegans] 0.0
blast CAE67889 Hypothetical protein CBG13485 [Caenorhabditis briggsae] 0.0
blast AAH86828 Vacuolar protein sorting protein 18 [Danio rerio] emb|CAI11898.1| vacuolar protein sorting protein 18 [Danio rerio] emb|CAI20654.1| vacuolar protein sorting protein 18 [Danio rerio] 4.0E-90
Protein Domains
Database ID Description E-Value
Pfam IPR007810 EMBL-EBI Pep3/Vps18/deep orange 7.1E-76
Pfam IPR007810 EMBL-EBI Pep3/Vps18/deep orange 7.1E-76
Pfam IPR007810 EMBL-EBI Pep3/Vps18/deep orange 7.1E-64
Sequence
Target Sequence 
>C. ELEGANS:184112_AT
caacgaatcgaatcatcttctgacaatacaagatcttcttccaattattccaaaattcac
tcgagttggtgctctcaaaccgattatcgtagactttttgaaacgaaacaaacaacgtct
cgaaaaactggaacgatcaatgaaagaagccactgaaattgcttcagaaatccgtgataa
acaagaaaaactgaaaaatcgaacgacagttgtgaagccgtcggatgtttgctcacattg
tgctcggccaatttctggaagagctttcaatgttcactcgtgccgtcattttttccatcg
agaatgccttgaaatcgctatgatttcatttttatcgcaggaagaagttgaaaaaatgaa
gacgctgataattgatgaagaacgagttttaagccaaatgaaagcagagcagttggctgg
aaatcagaaaggatttatcgagaaacaggagaaatatctgaaaattgcggcttttatctc
gaatattgttggagcagagtgcccattgtgtggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAACGAATCGAATCATCTTCTGACA 696 187 2451 Antisense
AATTCACTCGAGTTGGTGCTCTCAA 593 175 2504 Antisense
GTTGGTGCTCTCAAACCGATTATCG 224 437 2515 Antisense
TGAAGCCGTCGGATGTTTGCTCACA 231 575 2663 Antisense
TTTGCTCACATTGTGCTCGGCCAAT 22 669 2678 Antisense
TGTGCTCGGCCAATTTCTGGAAGAG 159 557 2689 Antisense
AGAGCTTTCAATGTTCACTCGTGCC 465 69 2710 Antisense
TGCCGTCATTTTTTCCATCGAGAAT 492 585 2731 Antisense
GAGAATGCCTTGAAATCGCTATGAT 565 391 2750 Antisense
AATTGCGGCTTTTATCTCGAATATT 47 179 2913 Antisense
GGAGCAGAGTGCCCATTGTGTGGAA 694 519 2941 Antisense
Sequence Source Affymetrix Proprietary Database