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[184111_s_at]

Description F26F4.7 /REP_DB=WormBase Gene ID /WP=CE01264 /TR=Q19818 /GB=AAA91222.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 57      4grp_total > 22      6grp_cgc > 38      6grp_cgc_sub > Da      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 271.2
(3)
P 		133.9
(-2.1)
P 		108.8
(-3)
A 		222.8
(1.2)
A 		162
B 197.2
(-0)
P 		158.9
(-1.2)
P 		102.4
(-3)
A 		292.7
(3)
P 		190
C 218.9
(2.4)
P 		135.8
(-3)
A 		140.6
(-2.7)
A 		227.5
(3)
P 		92
avr 229.1
(2.1) 		142.9
(-1.8) 		117.3
(-3) 		247.7
(3) 		130

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 163
1 2 3 4 5 6 range
A 52.8
(-1.6)
A 		45
(-2)
A 		31.2
(-2.8)
A 		27.6
(-3)
A 		70.3
(-0.6)
A 		133
(3)
M 		105
B 58.3
(-1.3)
A 		44
(-1.8)
A 		21.2
(-2.7)
A 		41.8
(-1.9)
A 		11.9
(-3)
A 		174.5
(3)
P 		163
C 78.3
(-0.5)
A 		34.6
(-2.5)
A 		63.6
(-1.1)
A 		33.3
(-2.5)
A 		23.4
(-3)
A 		153.4
(3)
P 		130
avr 63.1
(-1.5) 		41.2
(-2.6) 		38.7
(-2.8) 		34.2
(-3) 		35.2
(-2.9) 		153.6
(3) 		119
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 98.7
(3)
P 		29.1
(-2.9)
P 		28.4
(-3)
A 		32.6
(-2.6)
A 		70
B 62.9
(3)
P 		39.2
(-0.6)
P 		23.7
(-3)
A 		62.4
(2.9)
P 		39
C 72.5
(3)
P 		39
(-2.9)
A 		49
(-1.1)
A 		38.4
(-3)
P 		34
avr 78
(3) 		35.8
(-2.7) 		33.7
(-3) 		44.5
(-1.5) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 9.7
(-2.3)
A 		16
(-0.8)
A 		6.8
(-3)
A 		7
(-3)
A 		21.2
(0.4)
A 		32.4
(3)
M 		26
B 20.3
(-1.5)
A 		14.7
(-2)
A 		5.1
(-3)
A 		11.1
(-2.4)
A 		4.8
(-3)
A 		65.1
(3)
P 		60
C 29
(0)
A 		10.6
(-2.5)
A 		16.2
(-1.7)
A 		6.7
(-3)
A 		7.6
(-2.9)
A 		51.3
(3)
P 		45
avr 19.7
(-1.3) 		13.8
(-2.2) 		9.4
(-2.8) 		8.3
(-3) 		11.2
(-2.6) 		49.6
(3) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184111_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6482
Sequence Type Exemplar sequence
Representative Public ID F26F4.7 NCBI
Target Description F26F4.7 /REP_DB=WormBase Gene ID /WP=CE01264 /TR=Q19818 /GB=AAA91222.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065625(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065625 NCBI Caenorhabditis elegans NHL (ring finger b-box coiled coil) domain containing family member (nhl-2) (nhl-2) mRNA, complete cds. 11/11 None
SNAP00000005749 ENSEMBL cdna:SNAP chromosome:CEL140:III:4895116:4898918:-1 11/11 None
GENEFINDER00000005768 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4895116:4898918:-1 11/11 None
F26F4.7 ENSEMBL cdna:known chromosome:CEL140:III:4894608:4899046:-1 gene:F26F4.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4895115-4898918(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nhl-2
Ensembl F26F4.7
Entrez Gene 175654 Entrez gene
SwissProt Q19818 EMBL-EBI
WormBase CE29291 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1634020_AT dm
DROSGENOME1:152315_AT dm
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA91222 Nhl (ring finger b-box coiled coil) domain containing protein 2 [Caenorhabditis elegans] ref|NP_498026.1| NHL (ring finger b-box coiled coil) domain containing family member (nhl-2) [Caenorhabditis elegans] 0.0
blast CAE71093 Hypothetical protein CBG17943 [Caenorhabditis briggsae] 0.0
blast XP_624929 PREDICTED: similar to ENSANGP00000015377 [Apis mellifera] 9.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR000315 EMBL-EBI Zn-finger, B-box 7.0E-11
Pfam IPR000315 EMBL-EBI Zn-finger, B-box 7.4E-5
Pfam IPR001258 EMBL-EBI NHL repeat 8.2E-8
Pfam IPR001258 EMBL-EBI NHL repeat 3.0E-6
Pfam IPR001258 EMBL-EBI NHL repeat 6.0E-4
Pfam IPR001258 EMBL-EBI NHL repeat 0.0027
Sequence
Target Sequence 
>C. ELEGANS:184111_S_AT
cgtgctcagatcttcgaagctcgtggtgaattcgtcaaacgtctcaacatgatggctttg
gttccacgtggcggaatagaagtatccgctgctgccgcaaccccgaatggacaactttta
cttgttgatactgctggatttgtgtattctattgatgtagatgcaccacgagtcacattc
tggttcgatgcttccactcaactcggagaagcttctgatgttgccatgtttgataacctg
atttatataacggatttcaagcatcattgtgtccaagtttacacttctgaaggaaaattc
atccgaaaaatgggagaaccatcacagaccccatatccaattggaatagacgtctcgaag
gcaggagaggttcttgtagctgatactcatggaaatcatctgcacgttgttgtattctca
cccgaaggacaacatattcattcatttactcacaacgagtttagactttctcggtgcgtt
ggtctccgaatcgctaaaagtggtcacattgtgacgttgtgtaagcataaccacactcta
ttcgtcttcaaaccattgattcttccaaatggacttcctgttccaactc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTGCTCAGATCTTCGAAGCTCGTG 631 245 2512 Antisense
CATGATGGCTTTGGTTCCACGTGGC 30 207 2559 Antisense
GGAATAGAAGTATCCGCTGCTGCCG 616 533 2584 Antisense
GTCACATTCTGGTTCGATGCTTCCA 489 465 2683 Antisense
GGAGAAGCTTCTGATGTTGCCATGT 660 517 2716 Antisense
ATCACAGACCCCATATCCAATTGGA 11 27 2832 Antisense
CATCTGCACGTTGTTGTATTCTCAC 547 211 2908 Antisense
GTGCGTTGGTCTCCGAATCGCTAAA 43 475 2985 Antisense
GTAAGCATAACCACACTCTATTCGT 93 463 3032 Antisense
CACTCTATTCGTCTTCAAACCATTG 227 197 3045 Antisense
CCAAATGGACTTCCTGTTCCAACTC 583 275 3076 Antisense
Sequence Source Affymetrix Proprietary Database