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[184110_at]

Description Y87G2A.9 /REP_DB=WormBase Gene ID /WP=CE24688 /TR=Q9U1Q1 /GB=CAB60431.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 50      4grp_highest_8_8 > 25      4grp_high_8_8 > 60      6grp_highest_nonorm > 65      6grp_total > 42      4grp_total > 60      6grp_cgc > 90      6grp_high1_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 714
1 2 3 4 range
A 1014.1
(2)
P 		859.2
(0.4)
P 		1115.6
(3)
P 		516.8
(-3)
P 		599
B 1080.2
(1.6)
P 		1068.2
(1.5)
P 		1189
(3)
P 		706.2
(-3)
P 		483
C 1040.7
(1.1)
P 		1231.1
(3)
P 		1227.5
(3)
P 		629.6
(-3)
P 		602
avr 1045
(1.6) 		1052.8
(1.7) 		1177.4
(3) 		617.5
(-3) 		560

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 612
1 2 3 4 5 6 range
A 1116.9
(-0.8)
P 		1197
(0.2)
P 		1169.7
(-0.2)
P 		1422.8
(3)
P 		1004.2
(-2.3)
P 		945.3
(-3)
P 		478
B 1023.9
(-1)
P 		954.5
(-1.9)
P 		1045.7
(-0.7)
P 		991.6
(-1.4)
P 		1322.8
(3)
P 		872
(-3)
P 		451
C 1318.4
(1.4)
P 		942.8
(-2.3)
P 		1480.1
(3)
P 		1084.6
(-0.9)
P 		1199.1
(0.2)
P 		868
(-3)
P 		612
avr 1153.1
(1.6) 		1031.4
(-0.6) 		1231.8
(3) 		1166.3
(1.8) 		1175.4
(2) 		895.1
(-3) 		337
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 352
1 2 3 4 range
A 369.2
(3)
P 		186.5
(-0.7)
P 		291.6
(1.4)
P 		75.7
(-3)
P 		294
B 344.5
(3)
P 		263.8
(0.5)
P 		275.5
(0.9)
P 		150.5
(-3)
P 		194
C 344.5
(1.4)
P 		354
(1.6)
P 		427.8
(3)
P 		106.2
(-3)
P 		322
avr 352.7
(3) 		268.1
(0.9) 		331.6
(2.5) 		110.8
(-3) 		242

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 332
1 2 3 4 5 6 range
A 205.9
(-3)
P 		425.7
(3)
P 		254.7
(-1.7)
P 		360.9
(1.2)
P 		303
(-0.3)
P 		230.2
(-2.3)
P 		220
B 356.3
(-0.8)
P 		317.7
(-1.6)
P 		253.9
(-3)
P 		262.6
(-2.8)
P 		537.4
(3)
P 		325.2
(-1.5)
P 		284
C 488.7
(3)
P 		287.7
(-1.5)
P 		376.7
(0.5)
P 		218.1
(-3)
P 		388
(0.8)
P 		290.6
(-1.4)
P 		271
avr 350.3
(0.2) 		343.7
(-0.1) 		295.1
(-2.3) 		280.5
(-3) 		409.5
(3) 		282
(-2.9) 		129
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184110_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.625
Sequence Type Exemplar sequence
Representative Public ID Y87G2A.9 NCBI
Target Description Y87G2A.9 /REP_DB=WormBase Gene ID /WP=CE24688 /TR=Q9U1Q1 /GB=CAB60431.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060980(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060980 NCBI Caenorhabditis elegans UBiquitin Conjugating enzyme family member (ubc-14) (ubc-14) mRNA, complete cds. 11/11 None
GENEFINDER00000019610 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13589829:13593332:1 11/11 None
Y87G2A.9.2 ENSEMBL cdna:known chromosome:CEL140:I:13589815:13593399:1 gene:Y87G2A.9 11/11 None
Y87G2A.9.1 ENSEMBL cdna:known chromosome:CEL140:I:13589817:13593415:1 gene:Y87G2A.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019593 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13589759-13593263(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ubc-14
Ensembl Y87G2A.9
Entrez Gene 173228 Entrez gene
SwissProt Q9U1Q1 EMBL-EBI
WormBase CE24688 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:247712_AT ubiquitin-conjugating enzyme 7 (UBC7) at
CANINE:1589738_AT similar to ubiquitin-conjugating enzyme E2G 2 cfa
CANINE_2:CFA.11340.1.S1_AT similar to ubiquitin-conjugating enzyme E2G 2 cfa
CANINE_2:CFAAFFX.17233.1.S1_S_AT similar to ubiquitin-conjugating enzyme E2G 2 cfa
DROSOPHILA_2:1626313_AT courtless dm
DROSGENOME1:145193_AT courtless dm
HG-U133_PLUS_2:209041_S_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HG-U133A_2:209041_S_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HG-U133A:209041_S_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HG-FOCUS:209042_S_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HG-U133A_2:209042_S_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HG-U133A:209042_S_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HG-U133_PLUS_2:209042_S_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HU35KSUBA:W24013_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HU35KSUBB:RC_R08929_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HU35KSUBC:RC_AA449337_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HU35KSUBD:RC_AA443634_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
U133_X3P:G12804628_3P_X_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
U133_X3P:HS.283839.0.S1_3P_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
U133_X3P:HS.192853.0.A1_3P_A_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
U133_X3P:209042_3P_S_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HG-U133_PLUS_2:1557053_S_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HG-U133_PLUS_2:232755_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HG-U133B:232755_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HG-U95AV2:32236_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
HG-U95E:76889_AT ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) hs
MG-U74BV2:109685_AT ubiquitin-conjugating enzyme E2G 2 mm
MG-U74BV2:165331_AT ubiquitin-conjugating enzyme E2G 2 mm
MG-U74CV2:168987_R_AT ubiquitin-conjugating enzyme E2G 2 mm
MOE430A:1417033_AT ubiquitin-conjugating enzyme E2G 2 mm
MOUSE430A_2:1417033_AT ubiquitin-conjugating enzyme E2G 2 mm
MOUSE430_2:1417033_AT ubiquitin-conjugating enzyme E2G 2 mm
MOE430A:1417032_AT ubiquitin-conjugating enzyme E2G 2 mm
MOUSE430A_2:1417032_AT ubiquitin-conjugating enzyme E2G 2 mm
MOUSE430_2:1417032_AT ubiquitin-conjugating enzyme E2G 2 mm
MU19KSUBB:TC33995_AT Ubiquitin-conjugating enzyme E2G 2, mRNA (cDNA clone MGC:6521 IMAGE:2651046) mm
MU19KSUBC:TC36998_AT Ubiquitin-conjugating enzyme E2G 2, mRNA (cDNA clone MGC:6521 IMAGE:2651046) mm
RAT230_2:1371814_AT ubiquitin-conjugating enzyme E2G 2 (predicted) rn
RAE230A:1371814_AT ubiquitin-conjugating enzyme E2G 2 (predicted) rn
RG-U34A:RC_AA859722_AT ubiquitin-conjugating enzyme E2G 2 (predicted) rn
RAE230B:1380157_AT ubiquitin-conjugating enzyme E2G 2 (predicted) rn
RAT230_2:1380157_AT ubiquitin-conjugating enzyme E2G 2 (predicted) rn
YEAST_2:1776409_AT Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast T27470 hypothetical protein Y87G2A.r - Caenorhabditis elegans 1.0E-138
blast CAB60431 Hypothetical protein Y87G2A.9 [Caenorhabditis elegans] ref|NP_493381.1| UBiquitin Conjugating enzyme family member (ubc-14) [Caenorhabditis elegans] 7.0E-97
blast CAE63550 Hypothetical protein CBG08036 [Caenorhabditis briggsae] 2.0E-94
blast XP_625157 PREDICTED: similar to ENSANGP00000019471 [Apis mellifera] 3.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 6.7E-21
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 2.5E-55
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 1.5E-85
Sequence
Target Sequence 
>C. ELEGANS:184110_AT
gacaacccgtccaccagagggaataatcgccgctccaatcgatgaggacaactttttcga
atgggaatgtctcattacgggacccgaagagacgtgctttgcgaatggcgtatttccggc
gagaatcacttttccgcaggattatccgctatctccaccgaaaatgcgcttcacatgcgg
tatattccatccgaatatctacgcagacggacgtgtttgtatttcgattcttcatgcacc
gggagacgatccgactggttatgaattgtcgaatgaacgatggagtcctgttcaatcgat
tgagaagattttgctcagtgttgtcagcatgttggctgaaccaaacgacgaatcgccggc
gaacgtgagcgcggcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAACCCGTCCACCAGAGGGAATA 485 363 60 Antisense
TCGCCGCTCCAATCGATGAGGACAA 502 599 86 Antisense
GGAATGTCTCATTACGGGACCCGAA 103 533 123 Antisense
GACCCGAAGAGACGTGCTTTGCGAA 65 381 140 Antisense
TTGCGAATGGCGTATTTCCGGCGAG 265 701 158 Antisense
CCGGCGAGAATCACTTTTCCGCAGG 524 263 175 Antisense
TTCCGCAGGATTATCCGCTATCTCC 415 699 191 Antisense
GCTTCACATGCGGTATATTCCATCC 591 305 227 Antisense
ATTCCATCCGAATATCTACGCAGAC 585 7 243 Antisense
GATTCTTCATGCACCGGGAGACGAT 157 431 285 Antisense
TCGCCGGCGAACGTGAGCGCGGCAA 500 599 412 Antisense
Sequence Source Affymetrix Proprietary Database