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[184109_s_at]

Description F54D5.14 /REP_DB=WormBase Gene ID /WP=CE18730 /TR=Q9XTI9 /GB=CAA91339.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 86      6grp_highest_nonorm > 91      6grp_total > 77      4grp_total > 46      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 124
1 2 3 4 range
A 211.7
(3)
P 		104.9
(-3)
A 		112
(-2.6)
P 		126.5
(-1.8)
A 		107
B 204.8
(3)
P 		184.2
(1.6)
P 		146.6
(-0.9)
P 		115.2
(-3)
A 		90
C 228.8
(3)
P 		119.5
(-2.6)
M 		112.4
(-3)
P 		139
(-1.6)
M 		116
avr 215.1
(3) 		136.2
(-2.2) 		123.7
(-3) 		126.9
(-2.8) 		91

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 338
1 2 3 4 5 6 range
A 157.5
(-2.8)
P 		154.3
(-2.8)
P 		145.7
(-3)
P 		203.7
(-1.9)
P 		209.7
(-1.8)
P 		470.9
(3)
P 		325
B 176.4
(-2.7)
P 		165.5
(-3)
P 		192.3
(-2.4)
P 		176.6
(-2.7)
P 		177.3
(-2.7)
P 		414.1
(3)
P 		249
C 132.7
(-3)
P 		169.2
(-2.2)
P 		238.8
(-0.8)
P 		253
(-0.5)
P 		207.9
(-1.4)
P 		422.6
(3)
P 		290
avr 155.5
(-3) 		163
(-2.8) 		192.3
(-2.2) 		211.1
(-1.8) 		198.3
(-2.1) 		435.9
(3) 		280
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 77.1
(3)
P 		22.8
(-2.6)
A 		29.3
(-1.9)
P 		18.5
(-3)
A 		59
B 65.3
(3)
P 		45.5
(0.1)
P 		34
(-1.6)
P 		24.6
(-3)
A 		41
C 75.7
(3)
P 		34.3
(-1.7)
M 		39.2
(-1.2)
P 		23.4
(-3)
M 		52
avr 72.7
(3) 		34.2
(-1.6) 		34.2
(-1.6) 		22.2
(-3) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 29
(-3)
P 		54.9
(-1.2)
P 		31.7
(-2.8)
P 		51.7
(-1.4)
P 		63.3
(-0.6)
P 		114.7
(3)
P 		86
B 61.4
(-2.2)
P 		55.1
(-2.5)
P 		46.7
(-3)
P 		46.8
(-3)
P 		72
(-1.6)
P 		154.4
(3)
P 		108
C 49.2
(-3)
P 		51.6
(-2.8)
P 		60.8
(-2.2)
P 		50.9
(-2.9)
P 		67.3
(-1.8)
P 		141.5
(3)
P 		92
avr 46.5
(-3) 		53.9
(-2.5) 		46.4
(-3) 		49.8
(-2.8) 		67.5
(-1.6) 		136.9
(3) 		91
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184109_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3273
Sequence Type Exemplar sequence
Representative Public ID F54D5.14 NCBI
Target Description F54D5.14 /REP_DB=WormBase Gene ID /WP=CE18730 /TR=Q9XTI9 /GB=CAA91339.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064075(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064075 NCBI Caenorhabditis elegans F54D5.14 (F54D5.14) mRNA, complete cds. 11/11 None
SNAP00000019651 ENSEMBL cdna:SNAP chromosome:CEL140:II:11579116:11582384:1 11/11 None
GENEFINDER00000019654 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11579351:11582384:1 11/11 None
F54D5.14 ENSEMBL cdna:known chromosome:CEL140:II:11578619:11582632:1 gene:F54D5.14 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11578676-11582381(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54D5.14
Entrez Gene 174776 Entrez gene
SwissProt Q9XTI9 EMBL-EBI
WormBase CE18730 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91339 Hypothetical protein F54D5.14 [Caenorhabditis elegans] emb|CAB16920.1| Hypothetical protein F54D5.14 [Caenorhabditis elegans] ref|NP_496476.1| F54D5.14 [Caenorhabditis elegans] 0.0
blast CAE59470 Hypothetical protein CBG02854 [Caenorhabditis briggsae] 0.0
blast CAB01681 Hypothetical protein C23H4.6 [Caenorhabditis elegans] ref|NP_510041.1| C23H4.6 [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:184109_S_AT
aaaaatcttcatgcagcccgtgtaaaagcatatccgagcttgaaaaagttcacggaattg
aaggtgtgtaacaagttccaggagttgctcgcagttcgaggtcattttatcggtggtctc
gaattcgatcacgagaaggagacacttaatgtgaatgttcaatcttctaaagagaaggat
gcgatggctggacgaagacctgaagtccttgaggtagaagaagtcgacgagcattcgtat
gatgatgatagtgacgatagtaccggtccgcgtagaaagaagtccaagaagagcggccag
aaaaagaagagagttcgtgatctgaaaggattatccggaggagaacggtcttttgtgact
gccgcactcgtcatgtccttgtgggaagttatggagcagccattccgaatgatggatgag
tttgatgtctttatggacatgatgaatagaaagctggtgatggatttgctcgtcgagctg
gccaccaagaagttccctcacaatcagttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAATCTTCATGCAGCCCGTGTAA 299 129 2812 Antisense
CAGCCCGTGTAAAAGCATATCCGAG 212 203 2825 Antisense
AAGTTCCAGGAGTTGCTCGCAGTTC 553 155 2884 Antisense
GAGGTCATTTTATCGGTGGTCTCGA 315 403 2909 Antisense
TCGGTGGTCTCGAATTCGATCACGA 688 605 2921 Antisense
GGATGCGATGGCTGGACGAAGACCT 448 511 2988 Antisense
TAGTGACGATAGTACCGGTCCGCGT 276 647 3060 Antisense
AGAACGGTCTTTTGTGACTGCCGCA 597 73 3153 Antisense
CACTCGTCATGTCCTTGTGGGAAGT 295 197 3176 Antisense
GTTATGGAGCAGCCATTCCGAATGA 360 447 3199 Antisense
AAGAAGTTCCCTCACAATCAGTTCA 419 151 3298 Antisense
Sequence Source Affymetrix Proprietary Database