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[184085_at]

Description F25B4.2 /REP_DB=WormBase Gene ID /WP=CE09621 /TR=Q22967 /GB=AAB37081.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 19      6grp_high1_10_10 > 09      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 63      6grp_total > 91      4grp_total > 38      6grp_highest_sub > 05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 389
1 2 3 4 range
A 555.6
(3)
P 		289.2
(-1.4)
P 		194.3
(-3)
P 		357.1
(-0.3)
P 		361
B 551.3
(3)
P 		300.9
(-2)
P 		248
(-3)
P 		366.5
(-0.7)
P 		303
C 583.3
(3)
P 		418.6
(-0.6)
P 		365.3
(-1.7)
P 		305.8
(-3)
P 		278
avr 563.4
(3) 		336.2
(-1.6) 		269.2
(-3) 		343.1
(-1.5) 		294

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 590
1 2 3 4 5 6 range
A 347.4
(-2.5)
P 		499.3
(-0.6)
P 		364.9
(-2.3)
P 		781.8
(3)
P 		518.7
(-0.3)
P 		308.3
(-3)
P 		474
B 593.4
(0.7)
P 		420.5
(-1.1)
P 		232.2
(-3)
P 		578.9
(0.5)
P 		822
(3)
P 		375.9
(-1.5)
P 		590
C 757.1
(2.6)
P 		415.3
(-2.2)
P 		389.3
(-2.6)
P 		684.5
(1.6)
P 		784.2
(3)
P 		362
(-3)
P 		422
avr 566
(0.8) 		445
(-1.2) 		328.8
(-3) 		681.7
(2.6) 		708.3
(3) 		348.7
(-2.7) 		380
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 202.3
(3)
P 		62.8
(-2.5)
P 		50.8
(-3)
P 		52.3
(-2.9)
P 		152
B 175.8
(3)
P 		74.3
(-2.1)
P 		57.5
(-3)
P 		78.1
(-2)
P 		118
C 193.1
(3)
P 		120.3
(-0.1)
P 		127.3
(0.2)
P 		51.6
(-3)
P 		142
avr 190.4
(3) 		85.8
(-1.8) 		78.5
(-2.2) 		60.7
(-3) 		130

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 278
1 2 3 4 5 6 range
A 64
(-3)
P 		177.6
(2.1)
P 		79.5
(-2.3)
P 		198.3
(3)
P 		156.5
(1.1)
P 		75.1
(-2.5)
P 		134
B 206.5
(0.2)
P 		140
(-1.2)
P 		56.4
(-3)
P 		153.3
(-0.9)
P 		334
(3)
P 		140.2
(-1.2)
P 		278
C 280.6
(3)
P 		126.7
(-2.1)
P 		99.1
(-3)
P 		137.7
(-1.7)
P 		253.8
(2.1)
P 		121.2
(-2.3)
P 		182
avr 183.7
(0.7) 		148.1
(-0.5) 		78.3
(-3) 		163.1
(-0) 		248.1
(3) 		112.2
(-1.8) 		170
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184085_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12365
Sequence Type Exemplar sequence
Representative Public ID F25B4.2 NCBI
Target Description F25B4.2 /REP_DB=WormBase Gene ID /WP=CE09621 /TR=Q22967 /GB=AAB37081.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072100
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
F25B4.2 ENSEMBL cdna:known chromosome:CEL140:V:5712013:5714922:1 gene:F25B4.2 A 
SNAP00000033036 ENSEMBL cdna:SNAP chromosome:CEL140:V:5712017:5714533:1 None
NM_072100 NCBI Caenorhabditis elegans F25B4.2 (F25B4.2) mRNA, complete cds. A 
GENEFINDER00000033041 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5711543:5714533:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5712016-5715093(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25B4.2
Entrez Gene 178959 Entrez gene
SwissProt Q22967 EMBL-EBI
WormBase CE30760 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.23383.1.S1_AT similar to pellino 2 cfa
DROSOPHILA_2:1626980_AT Pellino dm
DROSGENOME1:154889_AT Pellino dm
HG-U133_PLUS_2:219132_AT pellino homolog 2 (Drosophila) hs
HG-U133A:219132_AT pellino homolog 2 (Drosophila) hs
HG-FOCUS:219132_AT pellino homolog 2 (Drosophila) hs
HG-U133A_2:219132_AT pellino homolog 2 (Drosophila) hs
HU35KSUBC:RC_N63226_AT pellino homolog 2 (Drosophila) hs
U133_X3P:G10864062_3P_AT pellino homolog 2 (Drosophila) hs
HG-U95C:62136_AT pellino homolog 2 (Drosophila) hs
U133_X3P:HS2.384645.1.S1_3P_AT Pellino homolog 2 (Drosophila) hs
HG-U133_PLUS_2:1561129_AT Pellino homolog 2 (Drosophila) hs
HG-U95B:42401_AT Pellino homolog 2 (Drosophila) hs
HU35KSUBD:RC_AA482008_AT Pellino homolog 2 (Drosophila) hs
MOE430A:1419006_S_AT pellino 2 mm
MOUSE430A_2:1419006_S_AT pellino 2 mm
MOUSE430_2:1419006_S_AT pellino 2 mm
MG-U74BV2:105315_AT pellino 2 mm
MG-U74CV2:167333_AT pellino 2 mm
MOE430B:1436966_AT pellino 2 mm
MOUSE430_2:1436966_AT pellino 2 mm
MG-U74CV2:135707_AT Pellino 2, mRNA (cDNA clone MGC:38603 IMAGE:5355240) mm
MOE430B:1437181_AT Pellino 2, mRNA (cDNA clone MGC:38603 IMAGE:5355240) mm
MOUSE430_2:1437181_AT Pellino 2, mRNA (cDNA clone MGC:38603 IMAGE:5355240) mm
MU19KSUBA:TC22403_AT Pellino 2, mRNA (cDNA clone MGC:38603 IMAGE:5355240) mm
RAE230B:1384706_AT similar to Pellino protein homolog 2 (Pellino 2) rn
RAT230_2:1384706_AT similar to Pellino protein homolog 2 (Pellino 2) rn
Protein Similarities
Method ID Description E-Value
blast AAB37081 Hypothetical protein F25B4.2 [Caenorhabditis elegans] ref|NP_504501.2| F25B4.2 [Caenorhabditis elegans] 0.0
blast CAB97346 Pellino protein [Caenorhabditis elegans] 0.0
blast CAE64584 Hypothetical protein CBG09337 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006800 EMBL-EBI Pellino 1.3E-15
Pfam IPR006800 EMBL-EBI Pellino 7.0E-126
Pfam IPR006800 EMBL-EBI Pellino 1.3E-15
Pfam IPR006800 EMBL-EBI Pellino 7.0E-126
Sequence
Target Sequence 
>C. ELEGANS:184085_AT
aagaccacaatgtccagttaacttgaatactcttgtgattccgaagaaaaggaatggacg
gcaaattaataggagacaaccatatgtgtatctccaatgcggacatgttcaaggaagaca
cgaatggggagttcaagagaatagtggtcaacgatcaggaaaatgtccgatttgtcttgt
ggaatcagagagaattgtgcagttatcaatgggaatggaaccatcatttcatctcgattc
tggtgtactagatcacacttttaatccatgtggacacatggcaagcaagcaaactgtctt
atactggtcccgcatccctctacctcaaggaacttgccgttacgacccagtttgtccatt
ctgctaccaacttttggcaacggaacgtccattctacaaacgatactccgatattacgtg
gtgcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGACCACAATGTCCAGTTAACTTG 645 151 930 Antisense
GTGTATCTCCAATGCGGACATGTTC 672 485 1015 Antisense
GAAAATGTCCGATTTGTCTTGTGGA 98 355 1088 Antisense
TGGAACCATCATTTCATCTCGATTC 352 547 1145 Antisense
TTTCATCTCGATTCTGGTGTACTAG 206 673 1156 Antisense
AGCAAACTGTCTTATACTGGTCCCG 266 75 1217 Antisense
CATCCCTCTACCTCAAGGAACTTGC 38 211 1242 Antisense
GTCCATTCTGCTACCAACTTTTGGC 515 477 1283 Antisense
TTGGCAACGGAACGTCCATTCTACA 263 707 1303 Antisense
GTCCATTCTACAAACGATACTCCGA 689 471 1316 Antisense
GATACTCCGATATTACGTGGTGCGT 16 425 1331 Antisense
Sequence Source Affymetrix Proprietary Database