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[184052_s_at]

Description F54H12.1 /REP_DB=WormBase Gene ID /WP=CE25005 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Aconitate hydratase
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 03      4grp_highest_8_8 > 42      4grp_high_8_8 > 49      6grp_highest_nonorm > 22      6grp_total > 34      4grp_total > 62      6grp_highest_sub > De      6grp_cgc > 34      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1890
1 2 3 4 range
A 2894.6
(3)
P 		2229.2
(0)
P 		2231
(0)
P 		1559.4
(-3)
P 		1335
B 3008.7
(3)
P 		1936.9
(-1.9)
P 		2290.3
(-0.3)
P 		1702.2
(-3)
P 		1307
C 2699.2
(3)
P 		1789.3
(-0.5)
P 		2109.4
(0.8)
P 		1118.6
(-3)
P 		1581
avr 2867.5
(3) 		1985.1
(-0.8) 		2210.2
(0.2) 		1460.1
(-3) 		1407

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1351
1 2 3 4 5 6 range
A 2259.6
(3)
P 		1992.9
(1.7)
P 		1840.8
(0.9)
P 		1065.9
(-3)
P 		1157.2
(-2.5)
P 		1336.2
(-1.6)
P 		1194
B 2349.7
(3)
P 		2177.3
(2.2)
P 		2040.3
(1.5)
P 		1409.9
(-1.5)
P 		1090.3
(-3)
P 		1217.9
(-2.4)
P 		1259
C 2195.1
(3)
P 		2103.8
(2.5)
P 		2084.4
(2.4)
P 		1464
(-0.7)
P 		998.3
(-3)
P 		1335
(-1.3)
P 		1197
avr 2268.1
(3) 		2091.3
(2.1) 		1988.5
(1.6) 		1313.3
(-1.8) 		1081.9
(-3) 		1296.4
(-1.9) 		1186
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 865
1 2 3 4 range
A 1053.9
(3)
P 		483.9
(-1.1)
P 		583.2
(-0.4)
P 		228.3
(-3)
P 		826
B 959.5
(3)
P 		478.4
(-1.8)
P 		530.7
(-1.3)
P 		362.7
(-3)
P 		597
C 893.5
(3)
P 		514.4
(-0.2)
P 		735.2
(1.7)
P 		188.6
(-3)
P 		705
avr 969
(3) 		492.2
(-1) 		616.4
(0) 		259.9
(-3) 		709

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 547
1 2 3 4 5 6 range
A 416.5
(-1)
P 		708.8
(3)
P 		400.9
(-1.2)
P 		270.3
(-3)
P 		349.2
(-1.9)
P 		325.3
(-2.2)
P 		439
B 817.6
(3)
P 		724.7
(1.7)
P 		495.4
(-1.4)
P 		373.4
(-3)
P 		443
(-2.1)
P 		454.1
(-1.9)
P 		444
C 813.6
(3)
P 		641.9
(1)
P 		530.5
(-0.3)
P 		294.4
(-3)
P 		323
(-2.7)
P 		446.9
(-1.2)
P 		519
avr 682.6
(2.9) 		691.8
(3) 		475.6
(-0.4) 		312.7
(-3) 		371.7
(-2.1) 		408.8
(-1.5) 		379
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184052_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7326
Sequence Type Exemplar sequence
Representative Public ID F54H12.1 NCBI
Target Description F54H12.1 /REP_DB=WormBase Gene ID /WP=CE25005 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Aconitate hydratase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171200(11), NM_171885(11), NM_066337(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171200 NCBI Caenorhabditis elegans ACOnitase family member (aco-2) (aco-2) mRNA, complete cds. 11/11 None
NM_171885 NCBI Caenorhabditis elegans ACOnitase family member (aco-2) (aco-2) mRNA, complete cds. 11/11 None
NM_066337 NCBI Caenorhabditis elegans ACOnitase family member (aco-2) (aco-2) mRNA, complete cds. 11/11 None
F54H12.1a ENSEMBL cdna:known chromosome:CEL140:III:7973641:7977249:1 gene:F54H12.1 11/11 None
F54H12.1b ENSEMBL cdna:known chromosome:CEL140:III:7973649:7976882:1 gene:F54H12.1 11/11 None
F54H12.1c ENSEMBL cdna:known chromosome:CEL140:III:7974487:7976882:1 gene:F54H12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7973646-7976880(+) 100.0 100.0
Public Domain and Genome References
Ensembl F54H12.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA28050 Aconitase protein 2, isoform a [Caenorhabditis elegans] ref|NP_741235.1| ACOnitase family member (aco-2) [Caenorhabditis elegans] sp|P34455|ACON_CAEEL Probable aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase) (Aconitase) 0.0
blast CAE75035 Hypothetical protein CBG22943 [Caenorhabditis briggsae] 0.0
blast AAN63393 Aconitase protein 2, isoform c [Caenorhabditis elegans] ref|NP_498738.2| ACOnitase family member (aco-2) [Caenorhabditis elegans] 0.0
blast AAL65788 Aconitase protein 2, isoform b [Caenorhabditis elegans] ref|NP_741236.1| ACOnitase family member (aco-2) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000573 EMBL-EBI Aconitate hydratase, C-terminal 8.5E-68
Pfam IPR001030 EMBL-EBI Aconitate hydratase, N-terminal 1.0E-126
Pfam IPR000573 EMBL-EBI Aconitate hydratase, C-terminal 8.5E-68
Pfam IPR001030 EMBL-EBI Aconitate hydratase, N-terminal 1.0E-19
Pfam IPR001030 EMBL-EBI Aconitate hydratase, N-terminal 1.0E-126
Pfam IPR000573 EMBL-EBI Aconitate hydratase, C-terminal 8.5E-68
Pfam IPR001030 EMBL-EBI Aconitate hydratase, N-terminal 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:184052_S_AT
tactgatcacatttctgctgctggaccatggctcaaatatcgtggacatttggacaacat
ttcgaataatcttttccttacggctatcaatgctgacaacggagagatgaacaaggtcaa
gaaccaagttactggagaatatggagccgttccagccactgcccgcaaatacaaggctga
tggagttagatgggttgctatcggagacgagaactacggagagggttcatctcgcgaaca
tgctgctcttgagccacgtcatcttggaggaagagctatcatcgtgaaatccttcgcccg
tattcacgagacgaacttgaagaaacaaggaatgctcccactcacatttgccaacccagc
tgactacgacaaaatcgatccatcagacaatgtttctatcgtcggactctccagttttgc
tccaggaaagccacttacggctattttcaagaagaccaatggatcaaaggtggaagtgac
cttgaatcacaccttcaacgagcaacaaatcgagtggttcaaggccggatctgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACTGATCACATTTCTGCTGCTGGA 238 647 1773 Antisense
GCTGCTGGACCATGGCTCAAATATC 302 297 1789 Antisense
GAATAATCTTTTCCTTACGGCTATC 555 325 1836 Antisense
AGAATATGGAGCCGTTCCAGCCACT 358 75 1908 Antisense
CAGCCACTGCCCGCAAATACAAGGC 194 203 1925 Antisense
AGAGGGTTCATCTCGCGAACATGCT 131 69 1992 Antisense
GAACATGCTGCTCTTGAGCCACGTC 341 351 2008 Antisense
AGCTATCATCGTGAAATCCTTCGCC 611 77 2046 Antisense
ACAATGTTTCTATCGTCGGACTCTC 56 115 2159 Antisense
ACTCTCCAGTTTTGCTCCAGGAAAG 255 99 2178 Antisense
TCGAGTGGTTCAAGGCCGGATCTGC 444 601 2282 Antisense
Sequence Source Affymetrix Proprietary Database