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[184050_s_at]

Description F44A2.5 /REP_DB=WormBase Gene ID /WP=CE04572 /TR=Q20378 /GB=AAA83444.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 06      4grp_high_8_8 > 15      6grp_highest_nonorm > 70      6grp_total > 06      4grp_total > 02      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 345
1 2 3 4 range
A 272
(-3)
P 		285.4
(-2.7)
P 		327.6
(-1.9)
P 		581.3
(3)
P 		309
B 314.8
(-1)
P 		242.4
(-2.8)
A 		236.1
(-3)
A 		466.9
(3)
P 		231
C 307.1
(-2.3)
P 		284.1
(-3)
P 		294.1
(-2.7)
M 		469
(3)
P 		185
avr 298
(-2.3) 		270.6
(-3) 		285.9
(-2.6) 		505.7
(3) 		235

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 298
1 2 3 4 5 6 range
A 423.3
(3)
P 		277.2
(-2.3)
P 		303
(-1.3)
P 		257
(-3)
P 		318.5
(-0.8)
P 		268.9
(-2.6)
P 		166
B 431.8
(3)
P 		335.5
(0.5)
P 		204.1
(-3)
P 		290
(-0.7)
P 		256.4
(-1.6)
P 		260
(-1.5)
P 		228
C 405.2
(3)
P 		343.4
(1.6)
P 		365.7
(2.1)
P 		282.4
(0.3)
P 		254.1
(-0.3)
P 		133.7
(-3)
P 		272
avr 420.1
(3) 		318.7
(-0.1) 		290.9
(-0.9) 		276.5
(-1.3) 		276.3
(-1.3) 		220.9
(-3) 		199
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 99
(3)
P 		62
(-3)
P 		85.6
(0.8)
P 		85.1
(0.7)
P 		37
B 100.4
(3)
P 		59.9
(-2.3)
A 		54.7
(-3)
A 		99.5
(2.9)
P 		46
C 101.7
(2.8)
P 		81.7
(-2.3)
P 		102.5
(3)
M 		79.1
(-3)
P 		23
avr 100.4
(3) 		67.9
(-3) 		80.9
(-0.6) 		87.9
(0.7) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 78
(-0.7)
P 		98.6
(3)
P 		66
(-2.9)
P 		65.2
(-3)
P 		96.1
(2.6)
P 		65.5
(-2.9)
P 		33
B 150.2
(3)
P 		111.7
(0.7)
P 		49.6
(-3)
P 		76.8
(-1.4)
P 		104.2
(0.3)
P 		97
(-0.2)
P 		101
C 150.2
(3)
P 		104.8
(0.4)
P 		93.1
(-0.3)
P 		56.8
(-2.3)
P 		82.2
(-0.9)
P 		44.8
(-3)
P 		105
avr 126.1
(3) 		105
(0.9) 		69.6
(-2.7) 		66.3
(-3) 		94.2
(-0.2) 		69.1
(-2.7) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184050_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12394
Sequence Type Exemplar sequence
Representative Public ID F44A2.5 NCBI
Target Description F44A2.5 /REP_DB=WormBase Gene ID /WP=CE04572 /TR=Q20378 /GB=AAA83444.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171504(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171504 NCBI Caenorhabditis elegans F44A2.5b (F44A2.5) mRNA, complete cds. 11/11 None
SNAP00000038849 ENSEMBL cdna:SNAP chromosome:CEL140:V:9302218:9304390:1 11/11 None
GENEFINDER00000038858 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9297915:9304390:1 11/11 None
F44A2.5a.2 ENSEMBL cdna:known chromosome:CEL140:V:9300216:9304735:1 gene:F44A2.5 11/11 None
F44A2.5b ENSEMBL cdna:known chromosome:CEL140:V:9300216:9304739:1 gene:F44A2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179310 2 1 NM_171504 184050_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9302143-9304390(+) 99.04 99.04
Public Domain and Genome References
Ensembl F44A2.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO12433 Hypothetical protein F44A2.5b [Caenorhabditis elegans] ref|NP_741589.2| F44A2.5b [Caenorhabditis elegans] 0.0
blast AAO12433 Hypothetical protein F44A2.5b [Caenorhabditis elegans] ref|NP_741589.2| F44A2.5b [Caenorhabditis elegans] 1.0E-178
blast T29786 hypothetical protein F44A2.5 - Caenorhabditis elegans 1.0E-171
blast T29786 hypothetical protein F44A2.5 - Caenorhabditis elegans 1.0E-168
blast CAE72149 Hypothetical protein CBG19247 [Caenorhabditis briggsae] 1.0E-166
blast CAE72149 Hypothetical protein CBG19247 [Caenorhabditis briggsae] 1.0E-165
Sequence
Target Sequence 
>C. ELEGANS:184050_S_AT
ttgagagcccttcaactacattcgcattcaattcaaattgagcaatggatcggtggagca
ttttgtgatgaagaacttgccgaatgtattggatccgctttatgccgtcatgcaattgaa
ggaggtggaggtagtggagtcggaaaattgtgtgctaactttaagtatgcaccatccatt
ggttcctttgtcaagggactaatcatcgccctctcccaatacttggaatgcaggcataag
attcgcgaagatcatttccgaatgtggatttcatttattcaattcctcacggaactctat
tcaaatttgggttctgattcaaaaggagaacttgccacaattgtgtttgatgtcttcaat
tatcttctccgtccaccaattctagaacatgtcaagattcaagaattggaatgtctcatt
tctattcttattaagggtggatacgatttggagagagaatgtcccgatcggttggcactg
ctaaaagatctgatcagggatgcattcattgacgtctccgaaccatgggcccgtaaaatg
attctccttctcttggaactcggtgcatctggatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGAGAGCCCTTCAACTACATTCGC 497 667 424 Antisense
TATTGGATCCGCTTTATGCCGTCAT 535 665 510 Antisense
TATGCACCATCCATTGGTTCCTTTG 389 657 589 Antisense
TCAAGGGACTAATCATCGCCCTCTC 203 633 614 Antisense
CGCCCTCTCCCAATACTTGGAATGC 203 257 630 Antisense
ATTCCTCACGGAACTCTATTCAAAT 322 7 705 Antisense
TGATGTCTTCAATTATCTTCTCCGT 38 567 771 Antisense
CTCCGTCCACCAATTCTAGAACATG 372 235 790 Antisense
GAGAATGTCCCGATCGGTTGGCACT 580 389 878 Antisense
TGGGCCCGTAAAATGATTCTCCTTC 77 553 949 Antisense
TCTTGGAACTCGGTGCATCTGGATG 462 617 974 Antisense
Sequence Source Affymetrix Proprietary Database