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[184033_s_at]

Description ZK250.5 /REP_DB=WormBase Gene ID /WP=CE15275 /TR=O17299 /GB=AAB71074.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 17      6grp_high1_10_10 > 61      4grp_high_8_8 > 26      6grp_highest_nonorm > 90      6grp_total > 61      4grp_total > 30      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 116.2
(3)
P 		64.5
(-3)
P 		65.1
(-2.9)
P 		71
(-2.2)
P 		52
B 74.3
(-0.2)
P 		64.2
(-1.4)
A 		50
(-3)
P 		102.9
(3)
P 		53
C 121.6
(3)
P 		91.8
(-0.5)
P 		71
(-3)
P 		74.2
(-2.6)
P 		51
avr 104
(3) 		73.5
(-1.4) 		62
(-3) 		82.7
(-0) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 68.9
(-1.5)
P 		96
(0.5)
P 		103.5
(1)
P 		131.8
(3)
P 		69
(-1.5)
P 		47.2
(-3)
P 		85
B 86.4
(-2.8)
P 		89.6
(-2.5)
P 		163.1
(3)
P 		107.9
(-1.2)
P 		107.2
(-1.2)
P 		83.6
(-3)
P 		80
C 102.2
(-0.4)
P 		106.4
(-0.2)
P 		87
(-1.4)
P 		156.8
(3)
P 		82.8
(-1.7)
P 		61.7
(-3)
P 		95
avr 85.8
(-1.1) 		97.3
(-0.1) 		117.9
(1.7) 		132.2
(3) 		86.3
(-1.1) 		64.2
(-3) 		68
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 42.3
(3)
P 		14
(-2.3)
P 		17
(-1.8)
P 		10.4
(-3)
P 		32
B 23.7
(3)
P 		15.8
(-0.9)
A 		11.6
(-3)
P 		21.9
(2.1)
P 		12
C 40.3
(3)
P 		26.4
(0)
P 		24.7
(-0.4)
P 		12.5
(-3)
P 		28
avr 35.4
(3) 		18.7
(-1.9) 		17.8
(-2.2) 		14.9
(-3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 12.7
(-2.7)
P 		34.1
(3)
P 		22.5
(-0.1)
P 		33.4
(2.8)
P 		20.8
(-0.5)
P 		11.5
(-3)
P 		23
B 30.1
(-2.4)
P 		29.8
(-2.5)
P 		39.6
(1.4)
P 		28.6
(-3)
P 		43.5
(3)
P 		31.2
(-2)
P 		15
C 37.9
(3)
P 		32.5
(1.1)
P 		22.1
(-2.5)
P 		31.5
(0.8)
P 		26.8
(-0.9)
P 		20.7
(-3)
P 		17
avr 26.9
(0.2) 		32.1
(3) 		28.1
(0.8) 		31.2
(2.5) 		30.4
(2.1) 		21.1
(-3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184033_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4389
Sequence Type Exemplar sequence
Representative Public ID ZK250.5 NCBI
Target Description ZK250.5 /REP_DB=WormBase Gene ID /WP=CE15275 /TR=O17299 /GB=AAB71074.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027348(11), NM_001027347(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027348 NCBI Caenorhabditis elegans ZK250.5b (ZK250.5) mRNA, complete cds. 11/11 None
NM_001027347 NCBI Caenorhabditis elegans ZK250.5a (ZK250.5) mRNA, complete cds. 11/11 None
SNAP00000013024 ENSEMBL cdna:SNAP chromosome:CEL140:II:1937136:1938259:-1 11/11 None
GENEFINDER00000013040 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1937136:1939745:-1 11/11 None
ZK250.5.1 ENSEMBL cdna:known chromosome:CEL140:II:1937088:1940148:-1 gene:ZK250.5 11/11 None
ZK250.5.2 ENSEMBL cdna:known chromosome:CEL140:II:1937136:1939751:-1 gene:ZK250.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1937134-1941670(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZK250.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAY43980 Hypothetical protein ZK250.5b [Caenorhabditis elegans] ref|NP_001022519.1| ZK250.5b [Caenorhabditis elegans] 0.0
blast AAB71074 Hypothetical protein ZK250.5a [Caenorhabditis elegans] ref|NP_001022518.1| ZK250.5a [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 3.7E-32
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 3.8E-6
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 7.1E-36
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 3.7E-32
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 3.8E-6
Pfam IPR002083 EMBL-EBI MATH 1.4E-22
Pfam IPR002083 EMBL-EBI MATH 5.6E-42
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 7.1E-36
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 3.7E-32
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 3.8E-6
Sequence
Target Sequence 
>C. ELEGANS:184033_S_AT
gttcagcaaatctatcttcttacgtggcgatttgattccagtagtggaattatgtttgag
aacattaagtttgaaagtagcggcgcaaatgctacaaaatgtggaccccgttgcaattaa
aacaattgaaattcgaccccctctcgggattccaattgccttacaattagagtcacactt
ggagcaacttgtatgcttggagcagtggacaagtgcacgtgaattgaagattgaacattt
tttcgtagactgtgatattgaaaatttgattcacttttcaaaggttgatataaggataac
atcaatttcattgaaagatgtcttatatctgaaaaagaaattcctattatcttccaattt
ccttcaattcaaggccaagttctcaaaaacaaatgtcgatgactcaatatttgaagacaa
ctttttgggagagccatttcgaagagttgaagatatatcatcaaatcaaccagatatttg
gttcttctcttaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCAGCAAATCTATCTTCTTACGT 568 443 1938 Antisense
GTGGCGATTTGATTCCAGTAGTGGA 273 491 1961 Antisense
GCTACAAAATGTGGACCCCGTTGCA 421 291 2028 Antisense
CTCTCGGGATTCCAATTGCCTTACA 592 227 2078 Antisense
ATTGCCTTACAATTAGAGTCACACT 608 5 2092 Antisense
GAGTCACACTTGGAGCAACTTGTAT 671 399 2107 Antisense
GGAGCAGTGGACAAGTGCACGTGAA 226 521 2136 Antisense
ATTCCTATTATCTTCCAATTTCCTT 655 7 2277 Antisense
TTCAATTCAAGGCCAAGTTCTCAAA 323 685 2300 Antisense
TGGGAGAGCCATTTCGAAGAGTTGA 183 553 2363 Antisense
CCAGATATTTGGTTCTTCTCTTACA 660 271 2407 Antisense
Sequence Source Affymetrix Proprietary Database