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[184023_at]

Description D1022.3 /REP_DB=WormBase Gene ID /WP=CE02577 /TR=Q18925 /GB=AAB93336.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 24      4grp_total > 14      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 164
1 2 3 4 range
A 200.1
(2.6)
P 		81.2
(-1.9)
A 		52
(-3)
A 		211.4
(3)
M 		159
B 139.8
(2.8)
P 		47.3
(-3)
A 		55.9
(-2.5)
A 		143.1
(3)
P 		96
C 125.1
(0.7)
P 		82.8
(-1.6)
A 		56.8
(-3)
A 		167.1
(3)
M 		110
avr 155
(2) 		70.4
(-2.2) 		54.9
(-3) 		173.9
(3) 		119

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 263
1 2 3 4 5 6 range
A 221.2
(1.4)
P 		280.1
(2.8)
P 		289.4
(3)
P 		78.5
(-2.1)
A 		90.4
(-1.8)
P 		41.4
(-3)
A 		248
B 297.1
(2.9)
P 		300.2
(3)
P 		222.8
(1.2)
P 		90.7
(-1.8)
P 		94.8
(-1.7)
P 		36.9
(-3)
A 		263
C 264.6
(3)
P 		226.7
(1.8)
P 		134.4
(-1.2)
P 		133.4
(-1.3)
P 		80.5
(-3)
P 		93.4
(-2.6)
A 		184
avr 261
(2.8) 		269
(3) 		215.5
(1.5) 		100.9
(-1.8) 		88.6
(-2.1) 		57.2
(-3) 		212
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 72.9
(3)
P 		17.6
(-2.6)
A 		13.6
(-3)
A 		30.9
(-1.2)
M 		59
B 44.6
(3)
P 		11.7
(-3)
A 		12.9
(-2.8)
A 		30.5
(0.4)
P 		33
C 41.4
(3)
P 		23.8
(-1.9)
A 		19.8
(-3)
A 		28.2
(-0.7)
M 		22
avr 53
(3) 		17.7
(-2.6) 		15.4
(-3) 		29.9
(-0.7) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 40.8
(-0.9)
P 		99.6
(3)
P 		63
(0.5)
P 		19.9
(-2.3)
A 		27.3
(-1.8)
P 		10.1
(-3)
A 		90
B 103.4
(3)
P 		99.9
(2.8)
P 		54.1
(-0.3)
P 		24
(-2.3)
P 		38.5
(-1.3)
P 		13.8
(-3)
A 		90
C 98.1
(3)
P 		69.2
(0.6)
P 		34.2
(-2.3)
P 		26.8
(-2.9)
P 		26
(-3)
P 		31.3
(-2.6)
A 		72
avr 80.8
(2.3) 		89.6
(3) 		50.4
(-0.3) 		23.6
(-2.6) 		30.6
(-2) 		18.4
(-3) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184023_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4464
Sequence Type Exemplar sequence
Representative Public ID D1022.3 NCBI
Target Description D1022.3 /REP_DB=WormBase Gene ID /WP=CE02577 /TR=Q18925 /GB=AAB93336.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063159(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063159 NCBI Caenorhabditis elegans D1022.3 (D1022.3) mRNA, complete cds. 11/11 A 
SNAP00000004643 ENSEMBL cdna:SNAP chromosome:CEL140:II:7455172:7456138:-1 11/11 None
GENEFINDER00000004660 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7455172:7456138:-1 11/11 None
D1022.3 ENSEMBL cdna:known chromosome:CEL140:II:7455172:7456138:-1 gene:D1022.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000023856 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7435076-7435711(+) 77.24 77.24
chrII:7455168-7456135(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl D1022.3
Entrez Gene 183900 Entrez gene
SwissProt Q18925 EMBL-EBI
WormBase CE02577 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_495560 D1022.3 [Caenorhabditis elegans] gb|AAB93336.1| Hypothetical protein D1022.3 [Caenorhabditis elegans] 1.0E-139
blast AAC46705 Hypothetical protein K02A2.1 [Caenorhabditis elegans] ref|NP_495556.1| K02A2.1 [Caenorhabditis elegans] sp|Q09571|YRD1_CAEEL Hypothetical protein K02A2.1 in chromosome II 4.0E-83
blast CAE67534 Hypothetical protein CBG13057 [Caenorhabditis briggsae] 3.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 1.1E-53
Sequence
Target Sequence 
>C. ELEGANS:184023_AT
attttccctgcattccgaggagtgtccaaactggaaattgcaagggcaaaattattggct
gaagaacatggatatgtagctcttgttgctgatatttatggaaaaggaattagatgcaca
gacatctcatcggcagtgacactgctccgtcctatgactagcgatagaaatggaaagttg
aaaccaagattagaagctgctttaaatgccttgaaatctgttccatgtgtagataaacag
aagctcggtgcatttggattctgtattggaggactgtgttccctggattgtgctcgttat
agatttgatggaattcgagctgttataagttttcatggaactctgacccctatcgaagga
attcctttagaacaattagatgatatttttattcaagttcaccatggagatgctgacgca
catgtttcaaaagtcaccgttgacgcattccacgaagaaatgagagcaagaaattccgac
tttgtttttatttctcatgggaaagcaatgcattcgttcactgatcctgagtctgctgaa
attgccccatctggagtcggttacgatgcgaatgctgacaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTTCCCTGCATTCCGAGGAGTGT 562 13 121 Antisense
GCATTCCGAGGAGTGTCCAAACTGG 479 307 130 Antisense
GTCCAAACTGGAAATTGCAAGGGCA 121 473 144 Antisense
CATGGATATGTAGCTCTTGTTGCTG 312 209 187 Antisense
GCTCTTGTTGCTGATATTTATGGAA 403 299 199 Antisense
GATGCACAGACATCTCATCGGCAGT 158 413 233 Antisense
CATCGGCAGTGACACTGCTCCGTCC 293 211 248 Antisense
TGCTCCGTCCTATGACTAGCGATAG 689 581 263 Antisense
ATGATATTTTTATTCAAGTTCACCA 376 47 500 Antisense
GGAGATGCTGACGCACATGTTTCAA 267 519 526 Antisense
GGTTACGATGCGAATGCTGACAAAA 632 503 679 Antisense
Sequence Source Affymetrix Proprietary Database