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[184018_at]

Description T14B4.8 /REP_DB=WormBase Gene ID /WP=CE02051 /TR=Q22480 /GB=AAK31554.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 13      4grp_high_8_8 > 26      6grp_total > 13      4grp_total > 37      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 246
1 2 3 4 range
A 253.3
(3)
P 		134.7
(-1.1)
P 		80.1
(-3)
A 		170.7
(0.1)
P 		173
B 278.9
(3)
P 		199.2
(0.9)
P 		56.3
(-3)
A 		165.3
(-0.1)
P 		223
C 302.7
(3)
P 		171
(-0.8)
P 		93
(-3)
P 		205.7
(0.2)
P 		210
avr 278.3
(3) 		168.3
(-0.3) 		76.5
(-3) 		180.6
(0.1) 		202

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 221
1 2 3 4 5 6 range
A 224.1
(1.3)
P 		272.6
(3)
P 		221.5
(1.2)
P 		160.2
(-1)
P 		191.4
(0.1)
P 		102.5
(-3)
P 		170
B 283.9
(3)
P 		259.7
(2.2)
P 		276.6
(2.8)
P 		186
(-0.1)
P 		130.4
(-1.9)
P 		96.8
(-3)
P 		187
C 302.7
(2.8)
P 		311.7
(3)
P 		177
(-0.7)
P 		209.6
(0.2)
P 		135
(-1.8)
P 		90.7
(-3)
M 		221
avr 270.2
(2.6) 		281.3
(3) 		225
(1.2) 		185.3
(-0.1) 		152.3
(-1.2) 		96.7
(-3) 		185
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 87
1 2 3 4 range
A 92.2
(3)
P 		29.2
(-2.3)
P 		20.9
(-3)
A 		25
(-2.7)
P 		71
B 89
(3)
P 		49.2
(-0.1)
P 		13
(-3)
A 		35.2
(-1.2)
P 		76
C 100.2
(3)
P 		49.2
(-1.5)
P 		32.4
(-3)
P 		34.7
(-2.8)
P 		68
avr 93.8
(3) 		42.5
(-1.3) 		22.1
(-3) 		31.6
(-2.2) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 41.3
(-1.6)
P 		96.9
(3)
P 		48.2
(-1.1)
P 		40.6
(-1.7)
P 		57.8
(-0.3)
P 		25
(-3)
P 		72
B 98.8
(3)
P 		86.4
(1.8)
P 		67.2
(-0)
P 		49.3
(-1.7)
P 		53
(-1.4)
P 		36.1
(-3)
P 		63
C 112.2
(3)
P 		95.1
(1.7)
P 		45
(-1.9)
P 		42.1
(-2.1)
P 		43.7
(-2)
P 		30.4
(-3)
M 		82
avr 84.1
(2.2) 		92.8
(3) 		53.5
(-0.8) 		44
(-1.7) 		51.5
(-1) 		30.5
(-3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184018_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4284
Sequence Type Exemplar sequence
Representative Public ID T14B4.8 NCBI
Target Description T14B4.8 /REP_DB=WormBase Gene ID /WP=CE02051 /TR=Q22480 /GB=AAK31554.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062969(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062969 NCBI Caenorhabditis elegans T14B4.8 (T14B4.8) mRNA, complete cds. 11/11 None
SNAP00000000077 ENSEMBL cdna:SNAP chromosome:CEL140:II:6720367:6721559:-1 11/11 None
GENEFINDER00000000087 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6720367:6723884:-1 11/11 None
T14B4.8 ENSEMBL cdna:known chromosome:CEL140:II:6720367:6722517:-1 gene:T14B4.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6720363-6722514(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T14B4.8
Entrez Gene 188493 Entrez gene
SwissProt Q22480 EMBL-EBI
WormBase CE02051 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495370 T14B4.8 [Caenorhabditis elegans] gb|AAK31554.1| Hypothetical protein T14B4.8 [Caenorhabditis elegans] 1.0E-177
blast CAE66037 Hypothetical protein CBG11233 [Caenorhabditis briggsae] 1.0E-151
blast NP_495370 T14B4.8 [Caenorhabditis elegans] gb|AAK31554.1| Hypothetical protein T14B4.8 [Caenorhabditis elegans] 1.0E-144
blast CAE66037 Hypothetical protein CBG11233 [Caenorhabditis briggsae] 1.0E-134
blast NP_495370 T14B4.8 [Caenorhabditis elegans] gb|AAK31554.1| Hypothetical protein T14B4.8 [Caenorhabditis elegans] 1.0E-134
blast CAE66037 Hypothetical protein CBG11233 [Caenorhabditis briggsae] 1.0E-124
Sequence
Target Sequence 
>C. ELEGANS:184018_AT
gttttattctacgtgtccacttcaacacatttacgattaaatcatcaaggatctgaatta
caagtaactgatccatcagactttccacttttagatatttggtcggaaaatgtttgtgat
gaagctcctttttggattattgaatcttacggtcgacgagtagcagttgtaacagacaca
gctcctcgatcaattctctcattctttattccatttctgcgacccaggcggactgtacca
actttttcaataattgattggaatggagaagttctaggatattatgcacttgcaagcgat
ggaatagaagttcaagatactcgccacgagcccattgcaaaagccttcaacgagcctgat
ccatctggaggatccacgtggcgagtcgtttgtgaaaagaactctcaacgacaactggca
gttctaacagctgatggacatttatcattttcatctgacattgctcttccacttaaactt
cttcttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTTATTCTACGTGTCCACTTCAA 331 449 376 Antisense
ATCCATCAGACTTTCCACTTTTAGA 454 39 446 Antisense
AGTTGTAACAGACACAGCTCCTCGA 285 63 540 Antisense
CTCGATCAATTCTCTCATTCTTTAT 466 233 560 Antisense
TCATTCTTTATTCCATTTCTGCGAC 581 619 574 Antisense
GCGGACTGTACCAACTTTTTCAATA 642 291 603 Antisense
AAGTTCAAGATACTCGCCACGAGCC 553 153 683 Antisense
CACGAGCCCATTGCAAAAGCCTTCA 83 201 700 Antisense
CAACGAGCCTGATCCATCTGGAGGA 618 187 723 Antisense
ATCCACGTGGCGAGTCGTTTGTGAA 389 37 747 Antisense
CTCTTCCACTTAAACTTCTTCTTAT 110 229 839 Antisense
Sequence Source Affymetrix Proprietary Database