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[184015_at]

Description R05F9.9 /REP_DB=WormBase Gene ID /WP=CE04812 /TR=Q21745 /GB=AAA83169.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 76      4grp_highest_8_8 > 45      4grp_high_8_8 > 0      6grp_highest_nonorm > 82      6grp_total > 79      4grp_total > 15      6grp_highest_sub > Da      6grp_high1_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 164
1 2 3 4 range
A 326.7
(1.4)
P 		285.4
(-3)
P 		293.2
(-2.2)
P 		341.9
(3)
P 		57
B 330.8
(1.8)
P 		319.6
(0.7)
P 		282.8
(-3)
P 		342.6
(3)
P 		60
C 377.2
(3)
P 		213.4
(-3)
P 		320.9
(0.9)
P 		306.4
(0.4)
P 		164
avr 344.9
(3) 		272.8
(-3) 		299
(-0.8) 		330.3
(1.8) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 238
1 2 3 4 5 6 range
A 332.1
(-3)
P 		431.7
(3)
P 		412.4
(1.8)
P 		348.6
(-2)
P 		402.6
(1.2)
P 		416.9
(2.1)
P 		100
B 366.9
(-1.6)
P 		423.6
(0.1)
P 		319.6
(-3)
P 		340.5
(-2.4)
P 		404.7
(-0.5)
P 		523.1
(3)
P 		204
C 337
(-2.4)
P 		449.9
(0.4)
P 		345.5
(-2.2)
P 		357.9
(-1.9)
P 		313.1
(-3)
P 		551.4
(3)
P 		238
avr 345.3
(-3) 		435.1
(0.5) 		359.2
(-2.5) 		349
(-2.9) 		373.5
(-1.9) 		497.1
(3) 		152
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 118.9
(3)
P 		62
(-2)
P 		76.7
(-0.7)
P 		50
(-3)
P 		69
B 105.5
(3)
P 		78.9
(-1)
P 		65.5
(-3)
P 		73
(-1.9)
P 		40
C 124.9
(3)
P 		61.4
(-2.2)
P 		111.8
(1.9)
P 		51.7
(-3)
P 		73
avr 116.4
(3) 		67.4
(-2.1) 		84.7
(-0.3) 		58.2
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 61.2
(-3)
P 		153.5
(3)
P 		89.8
(-1.1)
P 		88.4
(-1.2)
P 		121.5
(0.9)
P 		101.5
(-0.4)
P 		92
B 127.7
(-0.4)
P 		141
(0.2)
P 		77.6
(-3)
P 		90.2
(-2.4)
P 		164.4
(1.4)
P 		195.1
(3)
P 		118
C 124.9
(-0.2)
P 		137.3
(0.5)
P 		87.9
(-2.2)
P 		72
(-3)
P 		101.3
(-1.4)
P 		184.6
(3)
P 		113
avr 104.6
(-1.4) 		143.9
(1.7) 		85.1
(-2.9) 		83.5
(-3) 		129.1
(0.6) 		160.4
(3) 		77
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184015_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5905
Sequence Type Exemplar sequence
Representative Public ID R05F9.9 NCBI
Target Description R05F9.9 /REP_DB=WormBase Gene ID /WP=CE04812 /TR=Q21745 /GB=AAA83169.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062491(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062491 NCBI Caenorhabditis elegans R05F9.9 (R05F9.9) mRNA, complete cds. 11/11 None
SNAP00000043870 ENSEMBL cdna:SNAP chromosome:CEL140:II:4900135:4902085:-1 11/11 None
R05F9.9 ENSEMBL cdna:known chromosome:CEL140:II:4899920:4902085:-1 gene:R05F9.9 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000043879 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4900215-4902083(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R05F9.9
Entrez Gene 173845 Entrez gene
SwissProt Q21745 EMBL-EBI
WormBase CE04812 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494892 R05F9.9 [Caenorhabditis elegans] gb|AAA83169.1| Hypothetical protein R05F9.9 [Caenorhabditis elegans] 1.0E-151
blast NP_494892 R05F9.9 [Caenorhabditis elegans] gb|AAA83169.1| Hypothetical protein R05F9.9 [Caenorhabditis elegans] 1.0E-140
blast CAE58996 Hypothetical protein CBG02269 [Caenorhabditis briggsae] 3.0E-85
Sequence
Target Sequence 
>C. ELEGANS:184015_AT
aaaaatctgcttccgtattcgactttcgattgatgacagccaggaaccattgaatattgg
aattccattatcacaagttgaaagtgtttcaattaaagatcatagtgcgaaacatccaga
acctgctcttctttttttacttaagaaaggagctttagagagatttattcaggtctgtaa
tccactattgggcgaacttcacacatatcatggacgaaaacgtaaaatgctgacatatct
aacagtaattctcggaatggatccacacttggagcaaccgaatccagtttattgccgtgt
ttcattaatggctaacaaattcacattcccatttggaacagcgaagaaaattttattaaa
agatttaccaataatgtggcaagaagtcttggataaacaatacgatcgtcgtctgaaaac
tggaattcatcatgatagagaacgaatggcagagatggatgatgaagcatgggattggct
tatgtcatttctaaatgttaaatgggacaaatgtcccagtggtggacgttggttcgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAATCTGCTTCCGTATTCGACTT 584 129 174 Antisense
TTCGACTTTCGATTGATGACAGCCA 697 693 191 Antisense
GCGAAACATCCAGAACCTGCTCTTC 222 289 280 Antisense
TCAGGTCTGTAATCCACTATTGGGC 533 619 342 Antisense
GGGCGAACTTCACACATATCATGGA 242 493 363 Antisense
ATGGATCCACACTTGGAGCAACCGA 678 51 430 Antisense
AACCGAATCCAGTTTATTGCCGTGT 10 147 449 Antisense
GGCTAACAAATTCACATTCCCATTT 104 539 483 Antisense
TACGATCGTCGTCTGAAAACTGGAA 396 373 574 Antisense
GCATGGGATTGGCTTATGTCATTTC 150 311 640 Antisense
TCCCAGTGGTGGACGTTGGTTCGTT 542 595 687 Antisense
Sequence Source Affymetrix Proprietary Database