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[183995_s_at]

Description ZK250.6 /REP_DB=WormBase Gene ID /WP=CE15277 /TR=O17301 /GB=AAB71075.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 04      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 0.6
(-3)
A 		1.7
(1.4)
A 		1.2
(-0.6)
A 		2.1
(3)
A 		2
B 12
(3)
A 		2.9
(-2.3)
A 		1.6
(-3)
A 		8.2
(0.8)
A 		10
C 3.1
(-2.6)
A 		1.2
(-3)
A 		2.7
(-2.7)
A 		31.3
(3)
A 		30
avr 5.2
(-1.3) 		1.9
(-3) 		1.8
(-3) 		13.9
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 3.5
(-2.3)
A 		1
(-3)
A 		7.2
(-1.3)
A 		1.7
(-2.8)
A 		2.3
(-2.6)
A 		22.3
(3)
A 		21
B 8.3
(-0.3)
A 		17.6
(3)
A 		5.1
(-1.4)
A 		3.1
(-2.1)
A 		0.5
(-3)
A 		13.1
(1.4)
A 		17
C 2.6
(-2.1)
A 		3.9
(-1.5)
A 		3.4
(-1.7)
A 		0.9
(-3)
A 		12.8
(3)
A 		5.7
(-0.6)
A 		12
avr 4.8
(-1.5) 		7.5
(-0.2) 		5.2
(-1.3) 		1.9
(-3) 		5.2
(-1.3) 		13.7
(3) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 0.2
(-3)
A 		0.4
(3)
A 		0.3
(-0)
A 		0.3
(-0)
A 		0
B 3.8
(3)
A 		0.7
(-2.5)
A 		0.4
(-3)
A 		1.7
(-0.7)
A 		3
C 1
(-2.2)
A 		0.3
(-3)
A 		0.9
(-2.3)
A 		5.3
(3)
A 		5
avr 1.7
(0.8) 		0.5
(-3) 		0.5
(-3) 		2.4
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 0.6
(-2.8)
A 		0.4
(-3)
A 		1.6
(-1.6)
A 		0.4
(-3)
A 		0.7
(-2.6)
A 		5.4
(3)
A 		5
B 2.9
(-0.2)
A 		5.9
(3)
A 		1.2
(-1.9)
A 		0.8
(-2.4)
A 		0.2
(-3)
A 		4.9
(1.9)
A 		6
C 0.9
(-1.9)
A 		1.2
(-1.5)
A 		0.9
(-1.9)
A 		0.2
(-3)
A 		4.1
(3)
A 		1.9
(-0.4)
A 		4
avr 1.5
(-1.3) 		2.5
(0.3) 		1.2
(-1.8) 		0.5
(-3) 		1.7
(-1) 		4.1
(3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 183995_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4764
Sequence Type Exemplar sequence
Representative Public ID ZK250.6 NCBI
Target Description ZK250.6 /REP_DB=WormBase Gene ID /WP=CE15277 /TR=O17301 /GB=AAB71075.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027348(11), NM_061735(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027348 NCBI Caenorhabditis elegans ZK250.5b (ZK250.5) mRNA, complete cds. 11/11 None
NM_061735 NCBI Caenorhabditis elegans ZK250.6 (ZK250.6) mRNA, complete cds. 11/11 None
SNAP00000013025 ENSEMBL cdna:SNAP chromosome:CEL140:II:1940809:1941671:-1 11/11 None
SNAP00000013026 ENSEMBL cdna:SNAP chromosome:CEL140:II:1944059:1945169:-1 11/11 None
GENEFINDER00000013044 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1940372:1946518:-1 11/11 None
ZK250.6 ENSEMBL cdna:known chromosome:CEL140:II:1944059:1947912:-1 gene:ZK250.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000013027 1/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173560 2 1 NM_001027348 183995_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1943102-1947796(-) 94.32 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK250.6
Entrez Gene 191261 Entrez gene
SwissProt O17301 EMBL-EBI
WormBase CE28873 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAY43980 Hypothetical protein ZK250.5b [Caenorhabditis elegans] ref|NP_001022519.1| ZK250.5b [Caenorhabditis elegans] 0.0
blast AAB71074 Hypothetical protein ZK250.5a [Caenorhabditis elegans] ref|NP_001022518.1| ZK250.5a [Caenorhabditis elegans] 0.0
blast AAB71075 Hypothetical protein ZK250.6 [Caenorhabditis elegans] ref|NP_494136.1| ZK250.6 [Caenorhabditis elegans] 0.0
blast AAB71075 Hypothetical protein ZK250.6 [Caenorhabditis elegans] ref|NP_494136.1| ZK250.6 [Caenorhabditis elegans] 1.0E-165
blast AAF35424 Hypothetical protein C52E2.5 [Caenorhabditis elegans] ref|NP_494091.1| C52E2.5 [Caenorhabditis elegans] 1.0E-147
blast AAF60435 Hypothetical protein Y16E11A.1 [Caenorhabditis elegans] ref|NP_494144.2| Y16E11A.1 [Caenorhabditis elegans] 1.0E-128
blast AAF60435 Hypothetical protein Y16E11A.1 [Caenorhabditis elegans] ref|NP_494144.2| Y16E11A.1 [Caenorhabditis elegans] 1.0E-114
Protein Domains
Database ID Description E-Value
Pfam IPR002083 EMBL-EBI MATH 1.4E-22
Pfam IPR002083 EMBL-EBI MATH 5.6E-42
Pfam IPR002083 EMBL-EBI MATH 2.1E-51
Pfam IPR002083 EMBL-EBI MATH 2.1E-43
Pfam IPR002083 EMBL-EBI MATH 2.5E-45
Pfam IPR002083 EMBL-EBI MATH 6.4E-40
Pfam IPR002083 EMBL-EBI MATH 2.1E-51
Pfam IPR002083 EMBL-EBI MATH 2.1E-43
Pfam IPR002083 EMBL-EBI MATH 5.6E-42
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 7.1E-36
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 3.7E-32
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 3.8E-6
Pfam IPR002083 EMBL-EBI MATH 2.5E-45
Pfam IPR002083 EMBL-EBI MATH 6.4E-40
Pfam IPR002083 EMBL-EBI MATH 2.1E-51
Pfam IPR002083 EMBL-EBI MATH 2.1E-43
Sequence
Target Sequence 
>C. ELEGANS:183995_S_AT
aagttcgttttggtattccatggtgtctcgtgattcaaagaaaagatggatttttcaaat
tattcctacgatgctataaacaagcgtctagaatcaataattggtctatccgagttgagt
acacgctaaaattgatgtcagtagatggtcagagcctactattcaacacacaatacactt
tcaaagctggatatgatagcggctgggataaaataatcagatggaatgatatggaggaga
gatttatggttaacgacagtatcattattgaagttcgcgcgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTTCGTTTTGGTATTCCATGGTG 254 147 2192 Antisense
GTATTCCATGGTGTCTCGTGATTCA 249 453 2204 Antisense
CATGGTGTCTCGTGATTCAAAGAAA 93 209 2210 Antisense
TTCAAATTATTCCTACGATGCTATA 629 685 2245 Antisense
ACGATGCTATAAACAAGCGTCTAGA 150 91 2259 Antisense
GAATCAATAATTGGTCTATCCGAGT 129 327 2282 Antisense
GGTCTATCCGAGTTGAGTACACGCT 584 501 2294 Antisense
ATCCGAGTTGAGTACACGCTAAAAT 357 37 2299 Antisense
GATGTCAGTAGATGGTCAGAGCCTA 598 409 2325 Antisense
TGGTCAGAGCCTACTATTCAACACA 611 551 2337 Antisense
GTATCATTATTGAAGTTCGCGCGAA 203 455 2450 Antisense
Sequence Source Affymetrix Proprietary Database