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[183994_at]

Description ZK250.6 /REP_DB=WormBase Gene ID /WP=CE15277 /TR=O17301 /GB=AAB71075.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 01      4grp_total > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 2.1
(-2.2)
A 		4.1
(3)
A 		1.8
(-3)
A 		3.1
(0.4)
A 		2
B 3.6
(3)
A 		3.2
(2.1)
A 		2.3
(0.2)
A 		0.8
(-3)
A 		3
C 2.3
(-3)
A 		5.8
(-1.4)
A 		2.2
(-3)
A 		16
(3)
A 		14
avr 2.7
(-2.2) 		4.4
(0.1) 		2.1
(-3) 		6.6
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 5.4
(-2.1)
A 		29.3
(3)
A 		1.3
(-3)
A 		3
(-2.6)
A 		1.8
(-2.9)
A 		2.5
(-2.7)
A 		28
B 1.1
(-3)
A 		3
(1.1)
A 		3.9
(3)
A 		1.8
(-1.5)
A 		3
(1.1)
A 		1.7
(-1.7)
A 		3
C 0.7
(-2.6)
A 		0.5
(-3)
A 		1.1
(-1.8)
A 		3.4
(3)
A 		2.1
(0.3)
A 		1.5
(-0.9)
A 		3
avr 2.4
(-2.7) 		10.9
(3) 		2.1
(-2.9) 		2.7
(-2.5) 		2.3
(-2.7) 		1.9
(-3) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3
1 2 3 4 range
A 0.8
(1.8)
A 		0.9
(3)
A 		0.5
(-1.8)
A 		0.4
(-3)
A 		1
B 1.2
(3)
A 		0.8
(0.6)
A 		0.5
(-1.2)
A 		0.2
(-3)
A 		1
C 0.8
(-3)
A 		1.7
(-0.2)
A 		0.8
(-3)
A 		2.7
(3)
A 		2
avr 0.9
(0.6) 		1.1
(3) 		0.6
(-3) 		1.1
(3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 1
(-2.6)
A 		10.4
(3)
A 		0.3
(-3)
A 		0.8
(-2.7)
A 		0.5
(-2.9)
A 		0.6
(-2.8)
A 		10
B 0.4
(-3)
A 		1
(1.5)
A 		0.9
(0.8)
A 		0.5
(-2.3)
A 		1.2
(3)
A 		0.6
(-1.5)
A 		1
C 0.3
(-1.8)
A 		0.2
(-3)
A 		0.3
(-1.8)
A 		0.7
(3)
A 		0.7
(3)
A 		0.5
(0.6)
A 		1
avr 0.6
(-2.8) 		3.9
(3) 		0.5
(-3) 		0.7
(-2.6) 		0.8
(-2.5) 		0.6
(-2.8) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183994_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4764
Sequence Type Exemplar sequence
Representative Public ID ZK250.6 NCBI
Target Description ZK250.6 /REP_DB=WormBase Gene ID /WP=CE15277 /TR=O17301 /GB=AAB71075.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061735
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061735 NCBI Caenorhabditis elegans ZK250.6 (ZK250.6) mRNA, complete cds. A 
SNAP00000013028 ENSEMBL cdna:SNAP chromosome:CEL140:II:1947285:1947797:-1 None
GENEFINDER00000013037 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1947285:1947797:-1 None
ZK250.6 ENSEMBL cdna:known chromosome:CEL140:II:1944059:1947912:-1 gene:ZK250.6 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000013026 3/11 Cross Hyb Matching Probes None
GENEFINDER00000013044 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1943102-1947796(-) 94.32 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK250.6
Entrez Gene 191261 Entrez gene
SwissProt O17301 EMBL-EBI
WormBase CE28873 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB71075 Hypothetical protein ZK250.6 [Caenorhabditis elegans] ref|NP_494136.1| ZK250.6 [Caenorhabditis elegans] 0.0
blast AAF60435 Hypothetical protein Y16E11A.1 [Caenorhabditis elegans] ref|NP_494144.2| Y16E11A.1 [Caenorhabditis elegans] 1.0E-114
blast AAY43980 Hypothetical protein ZK250.5b [Caenorhabditis elegans] ref|NP_001022519.1| ZK250.5b [Caenorhabditis elegans] 1.0E-99
blast AAB71075 Hypothetical protein ZK250.6 [Caenorhabditis elegans] ref|NP_494136.1| ZK250.6 [Caenorhabditis elegans] 5.0E-84
blast AAK67220 Hypothetical protein C52E2.6 [Caenorhabditis elegans] ref|NP_494093.3| C52E2.6 [Caenorhabditis elegans] 3.0E-77
Protein Domains
Database ID Description E-Value
Pfam IPR002083 EMBL-EBI MATH 2.5E-45
Pfam IPR002083 EMBL-EBI MATH 6.4E-40
Pfam IPR002083 EMBL-EBI MATH 2.1E-51
Pfam IPR002083 EMBL-EBI MATH 2.1E-43
Sequence
Target Sequence 
>C. ELEGANS:183994_AT
ttcaacacacaatacactttcaaagctggatatgatagcggctgggataaaataatcaga
tggaatgatatggaggagagatttatggttaacgacagtatcattattgaagttcgcgcg
aagattgtagagatgactggatgtgaggatgatgtagatgatttgaacgaagagttctca
tttattatgcgaacgcgttcacgactcgaccaccgaagcgatgttttcgcccttccacca
ttgtggcaagacttctgttggcgccagagacaaaaatacactgttaaccataggaaatgc
actgatttcagtaataaattttcagatgaaaatttccagactcacagaggtcttccgccg
aaacaaattacagattttgaaagcctaagataccagctatcgcatgacacgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCAACACACAATACACTTTCAAAG 404 685 2353 Antisense
AGCTGGATATGATAGCGGCTGGGAT 300 81 2376 Antisense
GAGATTTATGGTTAACGACAGTATC 371 391 2430 Antisense
GAAGAGTTCTCATTTATTATGCGAA 348 339 2521 Antisense
TTATGCGAACGCGTTCACGACTCGA 466 681 2537 Antisense
CCTTCCACCATTGTGGCAAGACTTC 459 269 2583 Antisense
TTGTGGCAAGACTTCTGTTGGCGCC 170 709 2593 Antisense
GACTTCTGTTGGCGCCAGAGACAAA 602 371 2602 Antisense
AAATACACTGTTAACCATAGGAAAT 581 115 2626 Antisense
GATTTTGAAAGCCTAAGATACCAGC 292 429 2725 Antisense
AGATACCAGCTATCGCATGACACGT 209 67 2740 Antisense
Sequence Source Affymetrix Proprietary Database