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[183966_at]

Description F28B3.4 /REP_DB=WormBase Gene ID /WP=CE09742 /TR=O01784 /GB=AAK21372.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 29      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 54.1
(1.8)
A 		11.3
(-3)
A 		12.8
(-2.8)
A 		64.5
(3)
A 		53
B 21.8
(-1.2)
A 		58.2
(3)
A 		24.8
(-0.8)
A 		5.8
(-3)
A 		52
C 67.1
(2.3)
A 		34.5
(-2.3)
A 		29.5
(-3)
A 		72.1
(3)
A 		43
avr 47.7
(3) 		34.7
(-0.1) 		22.4
(-3) 		47.5
(3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 12.8
(-1.9)
A 		31.9
(1.7)
A 		7.1
(-3)
A 		23.7
(0.1)
A 		39
(3)
A 		16.8
(-1.2)
A 		32
B 37.8
(0.4)
A 		42.5
(1.7)
A 		47.4
(3)
A 		25
(-3)
A 		25.6
(-2.8)
A 		27.7
(-2.3)
A 		22
C 34.3
(0.1)
A 		20
(-3)
A 		25.8
(-1.7)
A 		31.3
(-0.5)
A 		47.5
(3)
A 		34.4
(0.1)
A 		28
avr 28.3
(-1.9) 		31.5
(-0.2) 		26.8
(-2.7) 		26.7
(-2.8) 		37.4
(3) 		26.3
(-3) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 19.7
(3)
A 		2.5
(-3)
A 		3.4
(-2.7)
A 		9.4
(-0.6)
A 		17
B 6.9
(-0.4)
A 		14.4
(3)
A 		5.7
(-1)
A 		1.2
(-3)
A 		13
C 22.2
(3)
A 		9.9
(-3)
A 		10.3
(-2.8)
A 		12.2
(-1.9)
A 		12
avr 16.3
(3) 		8.9
(-1.5) 		6.5
(-3) 		7.6
(-2.3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 2.4
(-2.5)
A 		11.4
(2.8)
A 		1.5
(-3)
A 		6
(-0.4)
A 		11.8
(3)
A 		4.1
(-1.5)
A 		10
B 13.1
(2.1)
A 		14.2
(3)
A 		11.5
(0.9)
A 		6.6
(-3)
A 		10.4
(0)
A 		10.3
(-0.1)
A 		8
C 12.7
(1.3)
A 		6.1
(-3)
A 		6.6
(-2.7)
A 		6.3
(-2.9)
A 		15.4
(3)
A 		11.5
(0.5)
A 		9
avr 9.4
(0) 		10.6
(1.2) 		6.5
(-2.8) 		6.3
(-3) 		12.5
(3) 		8.6
(-0.8) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183966_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.741
Sequence Type Exemplar sequence
Representative Public ID F28B3.4 NCBI
Target Description F28B3.4 /REP_DB=WormBase Gene ID /WP=CE09742 /TR=O01784 /GB=AAK21372.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059079(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059079 NCBI Caenorhabditis elegans F28B3.4 (F28B3.4) mRNA, complete cds. 11/11 None
F28B3.4 ENSEMBL cdna:known chromosome:CEL140:I:4934119:4935477:1 gene:F28B3.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000020903 7/11 Cross Hyb Matching Probes None
GENEFINDER00000020916 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4934118-4935477(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28B3.4
Entrez Gene 3565539 Entrez gene
SwissProt O01784 EMBL-EBI
WormBase CE09742 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK21372 Hypothetical protein F28B3.4 [Caenorhabditis elegans] ref|NP_491480.1| F28B3.4 [Caenorhabditis elegans] 1.0E-83
blast CAE73117 Hypothetical protein CBG20499 [Caenorhabditis briggsae] 1.0E-43
blast AAH68015 Hypothetical protein zgc:77348 [Danio rerio] ref|NP_998128.1| hypothetical protein LOC405899 [Danio rerio] 7.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR010506 EMBL-EBI DMAP1-binding 7.8E-40
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491480.1 1 7-29
F28B3.4 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:183966_AT
aattcatggctctttttgcttgttctccatccctttgacattcccttatatatttcgaaa
tcttgctctcttctgttgcagataatgaatgatccggacacactgcccgacgaagttcgg
gaaaagttggccgagctcgacttggagctgagcgagggggacatcaccaagaaggggtac
gataagaaacgggatgcgcttttggcgccttttaagagtctgcgggcagcaaatgcactt
cacgccgaacaatcatcagcttcaccgaattctcggtcaaatcgtagaaatcaacggaga
gttactcaagatgatgacagatatcattctgaaatccgagtagaagcagttcatcaagca
ttagccgagtattctgatggtcggaagcttggaccacaaccagtaaaaccgcatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTCATGGCTCTTTTTGCTTGTTC 64 177 73 Antisense
ATCCCTTTGACATTCCCTTATATAT 47 39 101 Antisense
ATTTCGAAATCTTGCTCTCTTCTGT 385 17 124 Antisense
AATGAATGATCCGGACACACTGCCC 89 165 156 Antisense
AAGTTGGCCGAGCTCGACTTGGAGC 199 155 196 Antisense
AAACGGGATGCGCTTTTGGCGCCTT 68 127 259 Antisense
GGCGCCTTTTAAGAGTCTGCGGGCA 677 505 276 Antisense
TGCGGGCAGCAAATGCACTTCACGC 357 581 293 Antisense
CAATCATCAGCTTCACCGAATTCTC 471 181 322 Antisense
GAAGCAGTTCATCAAGCATTAGCCG 616 341 415 Antisense
GGACCACAACCAGTAAAACCGCATC 297 519 463 Antisense
Sequence Source Affymetrix Proprietary Database