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[183955_at]

Description W04B5.3 /REP_DB=WormBase Gene ID /WP=CE20140 /TR=Q9UA61 /GB=AAC68916.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 03      4grp_high_8_8 > 60      6grp_highest_nonorm > 51      6grp_total > 24      4grp_total > 59      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 712
1 2 3 4 range
A 678.2
(1)
P 		903.7
(3)
P 		839.3
(2.4)
P 		241.3
(-3)
A 		662
B 629.5
(0.9)
P 		794.8
(2.3)
P 		872.3
(3)
P 		192.2
(-3)
P 		680
C 620
(1.1)
P 		704.2
(2)
P 		801.7
(3)
P 		218.1
(-3)
P 		584
avr 642.6
(1.1) 		800.9
(2.6) 		837.8
(3) 		217.2
(-3) 		621

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 532
1 2 3 4 5 6 range
A 683
(1.7)
P 		736.1
(3)
P 		588
(-0.7)
P 		497.7
(-3)
P 		595.4
(-0.5)
P 		518.7
(-2.5)
P 		238
B 816.9
(3)
P 		666
(1.2)
P 		641.5
(0.9)
P 		461.7
(-1.3)
P 		395.4
(-2.2)
P 		326.8
(-3)
P 		490
C 756.9
(2.2)
P 		575.3
(0.2)
P 		824.9
(3)
P 		412.5
(-1.7)
P 		530.4
(-0.3)
P 		292.8
(-3)
P 		532
avr 752.3
(3) 		659.1
(1.5) 		684.8
(1.9) 		457.3
(-1.7) 		507.1
(-0.9) 		379.4
(-3) 		373
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 244
1 2 3 4 range
A 246.9
(3)
P 		196.2
(1.6)
P 		219.4
(2.2)
P 		35.3
(-3)
A 		212
B 200.7
(2.9)
P 		196.3
(2.8)
P 		202.1
(3)
P 		41
(-3)
P 		161
C 205.2
(1.2)
P 		202.5
(1.1)
P 		279.4
(3)
P 		36.8
(-3)
P 		243
avr 217.6
(2.5) 		198.3
(1.9) 		233.6
(3) 		37.7
(-3) 		196

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 201
1 2 3 4 5 6 range
A 125.9
(-3)
P 		261.8
(3)
P 		128
(-2.9)
P 		126.2
(-3)
P 		179.7
(-0.6)
P 		126.3
(-3)
P 		136
B 284.2
(3)
P 		221.7
(0.7)
P 		155.8
(-1.7)
P 		122.3
(-3)
P 		160.6
(-1.6)
P 		121.9
(-3)
P 		162
C 280.6
(3)
P 		175.5
(-0.2)
P 		209.9
(0.9)
P 		83
(-3)
P 		171.6
(-0.3)
P 		98
(-2.5)
P 		198
avr 230.2
(3) 		219.7
(2.5) 		164.6
(-0.3) 		110.5
(-3) 		170.6
(0) 		115.4
(-2.8) 		120
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183955_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6707
Sequence Type Exemplar sequence
Representative Public ID W04B5.3 NCBI
Target Description W04B5.3 /REP_DB=WormBase Gene ID /WP=CE20140 /TR=Q9UA61 /GB=AAC68916.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171083(11), NM_065151(11), NM_171084(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171083 NCBI Caenorhabditis elegans W04B5.3b (W04B5.3) mRNA, complete cds. 11/11 A 
NM_065151 NCBI Caenorhabditis elegans W04B5.3c (W04B5.3) mRNA, complete cds. 11/11 None
NM_171084 NCBI Caenorhabditis elegans W04B5.3a (W04B5.3) mRNA, complete cds. 11/11 A 
SNAP00000019371 ENSEMBL cdna:SNAP chromosome:CEL140:III:2413473:2423847:1 11/11 None
GENEFINDER00000019378 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2419127:2423308:1 11/11 None
W04B5.3c.2 ENSEMBL cdna:known chromosome:CEL140:III:2418212:2422597:1 gene:W04B5.3 11/11 A 
W04B5.3b ENSEMBL cdna:known chromosome:CEL140:III:2418333:2423853:1 gene:W04B5.3 11/11 A 
W04B5.3c.1 ENSEMBL cdna:known chromosome:CEL140:III:2418553:2423890:1 gene:W04B5.3 11/11 None
W04B5.3a ENSEMBL cdna:known chromosome:CEL140:III:2418651:2423847:1 gene:W04B5.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2418650-2423794(+) 100.0 100.0
Public Domain and Genome References
Ensembl W04B5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL65795 Hypothetical protein W04B5.3b [Caenorhabditis elegans] ref|NP_741096.1| W04B5.3b [Caenorhabditis elegans] 1.0E-146
blast AAC68916 Hypothetical protein W04B5.3a [Caenorhabditis elegans] ref|NP_741097.1| W04B5.3a [Caenorhabditis elegans] 1.0E-144
blast AAC68916 Hypothetical protein W04B5.3a [Caenorhabditis elegans] ref|NP_741097.1| W04B5.3a [Caenorhabditis elegans] 1.0E-142
blast AAL65795 Hypothetical protein W04B5.3b [Caenorhabditis elegans] ref|NP_741096.1| W04B5.3b [Caenorhabditis elegans] 1.0E-142
blast AAC68916 Hypothetical protein W04B5.3a [Caenorhabditis elegans] ref|NP_741097.1| W04B5.3a [Caenorhabditis elegans] 1.0E-139
blast AAL65795 Hypothetical protein W04B5.3b [Caenorhabditis elegans] ref|NP_741096.1| W04B5.3b [Caenorhabditis elegans] 1.0E-139
Protein Domains
Database ID Description E-Value
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 4.0E-15
Sequence
Target Sequence 
>C. ELEGANS:183955_AT
agggattctcgaatcatccgtacccacttccgggattaatcgataattcgacacatgcat
cacagcagaatcccgttggaacattaccaccacaaacttccatgccaagtgcaccgccta
tcactcaggctccgccgactccggcgggagctgcgtacacctcgccatataccagcgtcc
ctatgccacagcctccgtcttccgttccactttcgtatacacctgcgccaactccatcgg
taccatatacagtgacctacccccaatcggctaattcaactccaggaattcctcagccac
ttccccaacaaatgtcacgtcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGGATTCTCGAATCATCCGTACCC 489 57 662 Antisense
GTACCCACTTCCGGGATTAATCGAT 290 459 681 Antisense
ATAATTCGACACATGCATCACAGCA 412 25 704 Antisense
ATCACAGCAGAATCCCGTTGGAACA 241 27 720 Antisense
ATCCCGTTGGAACATTACCACCACA 127 39 731 Antisense
GGAACATTACCACCACAAACTTCCA 140 529 739 Antisense
CACAAACTTCCATGCCAAGTGCACC 523 193 752 Antisense
CTCCATCGGTACCATATACAGTGAC 610 235 893 Antisense
GGTACCATATACAGTGACCTACCCC 65 505 900 Antisense
CCCAATCGGCTAATTCAACTCCAGG 297 257 923 Antisense
ACTTCCCCAACAAATGTCACGTCAA 234 103 960 Antisense
Sequence Source Affymetrix Proprietary Database