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[183953_at]

Description F58B6.2 /REP_DB=WormBase Gene ID /WP=CE19472 /TR=Q9TYU9 /GB=AAC78205.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 01      6grp_high1_10_10 > 0      4grp_high_8_8 > 45      6grp_highest_nonorm > 80      6grp_total > 09      4grp_total > 50      6grp_cgc > 0      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 188
1 2 3 4 range
A 156.9
(-0.2)
P 		245.1
(3)
P 		221.5
(2.2)
P 		77.1
(-3)
A 		168
B 107.3
(-2.3)
P 		265.2
(3)
P 		196.9
(0.7)
P 		85.3
(-3)
P 		180
C 124.8
(-2.7)
P 		246.6
(3)
P 		197.2
(0.7)
P 		119.1
(-3)
P 		128
avr 129.7
(-1.6) 		252.3
(3) 		205.2
(1.2) 		93.8
(-3) 		159

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 122
1 2 3 4 5 6 range
A 250.8
(3)
P 		160.9
(-2.7)
P 		156.5
(-3)
P 		205.7
(0.1)
P 		174.3
(-1.9)
P 		211.2
(0.5)
P 		94
B 205.8
(3)
P 		192.8
(2)
P 		165.2
(-0.2)
P 		193.1
(2)
P 		159.6
(-0.6)
P 		128.7
(-3)
P 		77
C 198.8
(-0.5)
P 		197.1
(-0.6)
P 		201.5
(-0.2)
P 		240.8
(3)
P 		183.5
(-1.7)
P 		168
(-3)
P 		73
avr 218.5
(3) 		183.6
(-1.3) 		174.4
(-2.4) 		213.2
(2.4) 		172.5
(-2.6) 		169.3
(-3) 		49
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 57.1
(2.9)
P 		53.2
(2.4)
P 		57.9
(3)
P 		11.3
(-3)
A 		47
B 34.2
(-1)
P 		65.5
(3)
P 		45.6
(0.5)
P 		18.2
(-3)
P 		47
C 41.3
(-0.5)
P 		70.9
(3)
P 		68.7
(2.7)
P 		20.1
(-3)
P 		51
avr 44.2
(0.6) 		63.2
(3) 		57.4
(2.3) 		16.5
(-3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 46.2
(0.1)
P 		57.2
(3)
P 		34.1
(-3)
P 		52.2
(1.7)
P 		52.6
(1.8)
P 		51.4
(1.5)
P 		23
B 71.6
(3)
P 		64.2
(1.6)
P 		40.1
(-3)
P 		51.1
(-0.9)
P 		64.8
(1.7)
P 		48
(-1.5)
P 		32
C 73.7
(3)
P 		60.1
(-0.2)
P 		51.3
(-2.3)
P 		48.4
(-3)
P 		59.4
(-0.4)
P 		56.2
(-1.2)
P 		25
avr 63.8
(3) 		60.5
(2.1) 		41.8
(-3) 		50.6
(-0.6) 		58.9
(1.7) 		51.9
(-0.2) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183953_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8113
Sequence Type Exemplar sequence
Representative Public ID F58B6.2 NCBI
Target Description F58B6.2 /REP_DB=WormBase Gene ID /WP=CE19472 /TR=Q9TYU9 /GB=AAC78205.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064933(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064933 NCBI Caenorhabditis elegans F58B6.2 (F58B6.2) mRNA, complete cds. 11/11 None
GENEFINDER00000003683 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1103212:1111830:1 11/11 None
F58B6.2 ENSEMBL cdna:known chromosome:CEL140:III:1103212:1111911:1 gene:F58B6.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000003676 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1103211-1111830(+) 98.94 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-268
Ensembl F58B6.2
Entrez Gene 175277 Entrez gene
SwissProt Q9TYU9 EMBL-EBI
WormBase CE19472 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.13054.1.S1_AT similar to CG33556-PA cfa
CHICKEN:GGAAFFX.6349.1.S1_S_AT similar to Arfaptin 1 (ADP-ribosylation factor interacting protein 1) gga
CHICKEN:GGAAFFX.6351.1.S1_AT similar to Arfaptin 1 (ADP-ribosylation factor interacting protein 1) gga
HU35KSUBA:RC_AA441818_AT KIAA1727 protein hs
HU35KSUBA:R81474_AT KIAA1727 protein hs
HU35KSUBD:RC_H03629_S_AT KIAA1727 protein hs
U133_X3P:HS.319946.0.S1_3P_AT KIAA1727 protein hs
HG-U133_PLUS_2:226599_AT KIAA1727 protein hs
HG-U133B:226599_AT KIAA1727 protein hs
HG-U95B:44096_AT KIAA1727 protein hs
HG-U95C:63729_F_AT KIAA1727 protein hs
HG-U133_PLUS_2:244333_AT KIAA1727 protein hs
HG-U133B:244333_AT KIAA1727 protein hs
HG-U95D:73584_AT KIAA1727 protein hs
HG-U95D:78636_AT KIAA1727 protein hs
HG-U95D:90828_AT KIAA1727 protein hs
U133_X3P:HS.132629.0.A1_3P_AT KIAA1727 protein hs
MU11KSUBA:AA423082_AT RIKEN cDNA 6330505N24 gene (6330505N24Rik), mRNA mm
MOE430B:1443657_AT RIKEN cDNA 6330505N24 gene (6330505N24Rik), mRNA mm
MOUSE430_2:1443657_AT RIKEN cDNA 6330505N24 gene (6330505N24Rik), mRNA mm
RAE230A:1372086_AT similar to CG32384-PA (predicted) rn
RAT230_2:1372086_AT similar to CG32384-PA (predicted) rn
RG-U34B:RC_AI030543_R_AT similar to CG32384-PA (predicted) rn
RG-U34B:RC_AI030543_S_AT similar to CG32384-PA (predicted) rn
RG-U34B:RC_AI013605_AT similar to CG32384-PA (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3779 actin binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC78205 Temporarily assigned gene name protein 268 [Caenorhabditis elegans] ref|NP_497334.1| F58B6.2 [Caenorhabditis elegans] 0.0
blast CAE69130 Hypothetical protein CBG15157 [Caenorhabditis briggsae] 0.0
blast XP_793426 PREDICTED: similar to CG33556-PA [Strongylocentrotus purpuratus] 5.0E-43
blast XP_793426 PREDICTED: similar to CG33556-PA [Strongylocentrotus purpuratus] 7.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR003104 EMBL-EBI Actin-binding FH2 2.0E-105
Pfam IPR003104 EMBL-EBI Actin-binding FH2 2.3E-108
Sequence
Target Sequence 
>C. ELEGANS:183953_AT
aatcgataaaagccccgtctacagtaaccacccgatcgaaagttccaccgccgacagcgg
ctgcggcggtccgtattgtcagtgtgacgaccacaacaactccaaccaaaacggcagagc
tcaggaaaccgggcgctcggagcccgaagactacagtagctacagtaccaaaggttactg
tagttcctgtgagcagagtacctgtggctccgtcaactccgctgagccgacgaatgagtg
ctccagtagtccggaagccaactatgacggctgagaaaaaacgggagataaccatgaagc
cgtcggtatcaacatctgcacgtcctagcctcatcaataccagctcccacccgatggtga
ggtccccactgcctaaaatgagtgttctggagaagccgaagccactgcggatcacccgac
ctacagtaattccccagtcgcctacagtgacgtcatcggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCGATAAAAGCCCCGTCTACAGT 330 169 2153 Antisense
CTACAGTAACCACCCGATCGAAAGT 587 225 2171 Antisense
CACCGCCGACAGCGGCTGCGGCGGT 199 201 2198 Antisense
GGCGGTCCGTATTGTCAGTGTGACG 452 541 2217 Antisense
GCAGAGCTCAGGAAACCGGGCGCTC 105 311 2266 Antisense
CTCGGAGCCCGAAGACTACAGTAGC 229 233 2288 Antisense
TGAGCAGAGTACCTGTGGCTCCGTC 556 569 2342 Antisense
TGAGTGCTCCAGTAGTCCGGAAGCC 66 569 2387 Antisense
CGTCGGTATCAACATCTGCACGTCC 180 247 2453 Antisense
GGATCACCCGACCTACAGTAATTCC 194 511 2561 Antisense
AGTCGCCTACAGTGACGTCATCGGC 417 59 2588 Antisense
Sequence Source Affymetrix Proprietary Database