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[183951_at]

Description C33H5.7 /REP_DB=WormBase Gene ID /WP=CE04150 /TR=Q18403 /GB=AAA82264.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 85      4grp_highest_8_8 > 32      4grp_high_8_8 > 43      6grp_highest_nonorm > 83      6grp_total > 76      4grp_total > 52      6grp_highest_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 343
(-1.3)
P 		458.4
(3)
P 		356.1
(-0.8)
P 		298.1
(-3)
P 		160
B 452
(3)
P 		409.8
(-0.3)
P 		374.8
(-3)
P 		379.6
(-2.6)
P 		77
C 392.1
(0.1)
P 		366.5
(-2.1)
P 		424.8
(3)
P 		356.8
(-3)
P 		68
avr 395.7
(1.6) 		411.6
(3) 		385.2
(0.6) 		344.8
(-3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 391
1 2 3 4 5 6 range
A 303
(-3)
P 		376.7
(-1.3)
P 		453
(0.4)
P 		517.8
(1.9)
P 		440.4
(0.1)
P 		566.2
(3)
P 		263
B 362.7
(-3)
P 		395.8
(-2.4)
P 		469.1
(-1.1)
P 		419
(-2)
P 		456.8
(-1.3)
P 		694.4
(3)
P 		332
C 340.4
(-3)
P 		359
(-2.7)
P 		424.8
(-1.5)
P 		476.6
(-0.5)
P 		409.5
(-1.8)
P 		673.9
(3)
P 		334
avr 335.4
(-3) 		377.2
(-2.2) 		449
(-0.8) 		471.1
(-0.4) 		435.6
(-1.1) 		644.8
(3) 		309
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 124.9
(3)
P 		99.5
(1.1)
P 		93.1
(0.7)
P 		43.6
(-3)
P 		81
B 144.1
(3)
P 		101.2
(-1.1)
P 		86.9
(-2.4)
P 		80.9
(-3)
P 		63
C 129.8
(1.8)
P 		105.4
(0.1)
P 		148
(3)
P 		60.2
(-3)
P 		88
avr 132.9
(3) 		102
(0.4) 		109.3
(1) 		61.6
(-3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 55.9
(-3)
P 		134
(2.7)
P 		98.7
(0.1)
P 		131.3
(2.5)
P 		132.9
(2.6)
P 		137.9
(3)
P 		82
B 126.2
(-2.4)
P 		131.7
(-2.2)
P 		113.9
(-2.9)
P 		111
(-3)
P 		185.6
(0)
P 		259
(3)
P 		148
C 126.2
(-1.6)
P 		109.5
(-2.4)
P 		108.1
(-2.4)
P 		95.8
(-3)
P 		132.5
(-1.3)
P 		225.6
(3)
P 		130
avr 102.8
(-3) 		125.1
(-1.7) 		106.9
(-2.8) 		112.7
(-2.4) 		150.3
(-0.3) 		207.5
(3) 		105
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183951_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9623
Sequence Type Exemplar sequence
Representative Public ID C33H5.7 NCBI
Target Description C33H5.7 /REP_DB=WormBase Gene ID /WP=CE04150 /TR=Q18403 /GB=AAA82264.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068879(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068879 NCBI Caenorhabditis elegans C33H5.7 (C33H5.7) mRNA, complete cds. 11/11 A 
SNAP00000004474 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7774524:7775770:1 11/11 None
GENEFINDER00000004499 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7774524:7775770:1 11/11 None
C33H5.7 ENSEMBL cdna:known chromosome:CEL140:IV:7774431:7775770:1 gene:C33H5.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7774529-7775776(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C33H5.7
Entrez Gene 177561 Entrez gene
SwissProt Q18403 EMBL-EBI
WormBase CE04150 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250148_AT transducin family protein / WD-40 repeat family protein at
CANINE:1605550_AT similar to twinfilin-like protein cfa
CANINE_2:CFA.19770.1.S1_S_AT similar to twinfilin-like protein cfa
CANINE_2:CFA.19842.1.S1_S_AT similar to twinfilin-like protein cfa
CANINE_2:CFAAFFX.15456.1.S1_X_AT similar to twinfilin-like protein cfa
DROSOPHILA_2:1627048_AT dm
DROSGENOME1:152257_AT dm
CHICKEN:GGA.4765.1.S1_AT similar to CG17293-PA gga
CHICKEN:GGAAFFX.11380.1.S1_S_AT similar to CG17293-PA gga
MG-U74AV2:96924_AT RIKEN cDNA 9430077D24 gene mm
MOE430A:1452189_AT RIKEN cDNA 9430077D24 gene mm
MOUSE430A_2:1452189_AT RIKEN cDNA 9430077D24 gene mm
MOUSE430_2:1452189_AT RIKEN cDNA 9430077D24 gene mm
MG-U74BV2:112094_AT RIKEN cDNA 9430077D24 gene, mRNA (cDNA clone IMAGE:3596090) mm
MU19KSUBB:TC26763_AT RIKEN cDNA 9430077D24 gene, mRNA (cDNA clone IMAGE:3596090) mm
MU19KSUBB:TC26763_G_AT RIKEN cDNA 9430077D24 gene, mRNA (cDNA clone IMAGE:3596090) mm
YEAST_2:1778867_AT Subunit of the COMPASS (Set1C) complex, which methylates ubiquitinated histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Sc
Protein Similarities
Method ID Description E-Value
blast AAA82264 Hypothetical protein C33H5.7 [Caenorhabditis elegans] ref|NP_501280.1| C33H5.7 [Caenorhabditis elegans] 0.0
blast CAE70879 Hypothetical protein CBG17669 [Caenorhabditis briggsae] 1.0E-145
blast AAF52654 CG17293-PA [Drosophila melanogaster] gb|AAL39226.1| GH09638p [Drosophila melanogaster] ref|NP_609217.1| CG17293-PA [Drosophila melanogaster] 9.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.0E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.64
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.11
Sequence
Target Sequence 
>C. ELEGANS:183951_AT
ggacgatatgtttctctcagttgccgaagacaaaaccattcgactttgggatctgaaaac
tcataattgcattggattaatgaatctttcggcaactcctatagcaacatttgatccaga
aggacttctttttgcagcagggcttgacaataacattatcaaactttatgatttacgatc
tttcgacaaaggtccattttctagttttggtcctttggaaaacgaagacaacgtggaatg
gactgcaatgcgattctcgccatgtggaaaatatataatgatttgtacaaattcatcaca
gttattacttgtggatgcattcacgggactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACGATATGTTTCTCTCAGTTGCC 186 523 381 Antisense
GTTTCTCTCAGTTGCCGAAGACAAA 115 451 390 Antisense
AAACCATTCGACTTTGGGATCTGAA 395 125 413 Antisense
GAATCTTTCGGCAACTCCTATAGCA 190 329 462 Antisense
ACTCCTATAGCAACATTTGATCCAG 86 99 475 Antisense
AGGACTTCTTTTTGCAGCAGGGCTT 430 55 501 Antisense
ATCTTTCGACAAAGGTCCATTTTCT 570 31 558 Antisense
TCCATTTTCTAGTTTTGGTCCTTTG 648 589 573 Antisense
GACAACGTGGAATGGACTGCAATGC 210 365 607 Antisense
GACTGCAATGCGATTCTCGCCATGT 329 375 621 Antisense
ACTTGTGGATGCATTCACGGGACTT 245 105 687 Antisense
Sequence Source Affymetrix Proprietary Database