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[183938_at]

Description F46F5.4 /REP_DB=WormBase Gene ID /WP=CE19420 /TR=Q9TXX7 /GB=AAC78184.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 19      4grp_total > 35      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 3.5
(-3)
A 		54.4
(3)
A 		6.3
(-2.7)
A 		40.2
(1.3)
A 		51
B 20.3
(0.3)
A 		33.2
(3)
A 		4.9
(-3)
A 		26.5
(1.6)
A 		28
C 30.8
(2.9)
A 		26
(1.7)
A 		31.2
(3)
A 		6.4
(-3)
A 		25
avr 18.2
(-2) 		37.9
(3) 		14.1
(-3) 		24.4
(-0.4) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 54.2
(3)
A 		2.9
(-3)
A 		2.5
(-3)
A 		27.7
(-0.1)
A 		27.5
(-0.1)
A 		12.1
(-1.9)
A 		52
B 15.2
(-1.3)
A 		48.8
(3)
M 		1.9
(-3)
A 		22.9
(-0.3)
A 		2.1
(-3)
A 		18.4
(-0.9)
A 		47
C 31
(3)
A 		5.4
(-2.3)
A 		2.1
(-3)
A 		19.2
(0.6)
A 		15.7
(-0.2)
A 		3.2
(-2.8)
A 		29
avr 33.5
(3) 		19
(0.2) 		2.2
(-3) 		23.3
(1) 		15.1
(-0.5) 		11.2
(-1.3) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 1.3
(-3)
A 		11.8
(3)
A 		1.7
(-2.8)
A 		5.9
(-0.4)
A 		11
B 6.5
(1.6)
A 		8.2
(3)
A 		1.1
(-3)
A 		5.6
(0.8)
A 		7
C 10.2
(2.6)
A 		7.5
(0.9)
A 		10.9
(3)
A 		1.1
(-3)
A 		10
avr 6
(-0.8) 		9.2
(3) 		4.6
(-2.5) 		4.2
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 10
(3)
A 		1
(-2.7)
A 		0.5
(-3)
A 		7
(1.1)
A 		8.3
(1.9)
A 		3
(-1.4)
A 		10
B 5.3
(-1.2)
A 		16.3
(3)
M 		0.5
(-3)
A 		6.1
(-0.9)
A 		0.8
(-2.9)
A 		6.9
(-0.6)
A 		16
C 11.5
(3)
A 		1.7
(-2.3)
A 		0.5
(-3)
A 		3.9
(-1.1)
A 		5.1
(-0.5)
A 		1.1
(-2.7)
A 		11
avr 8.9
(3) 		6.3
(1.1) 		0.5
(-3) 		5.7
(0.7) 		4.7
(0) 		3.7
(-0.7) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183938_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5820
Sequence Type Exemplar sequence
Representative Public ID F46F5.4 NCBI
Target Description F46F5.4 /REP_DB=WormBase Gene ID /WP=CE19420 /TR=Q9TXX7 /GB=AAC78184.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061414(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061414 NCBI Caenorhabditis elegans F46F5.4 (F46F5.4) mRNA, complete cds. 11/11 None
SNAP00000029518 ENSEMBL cdna:SNAP chromosome:CEL140:II:805291:806124:-1 9/11 None
GENEFINDER00000029532 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:805036:806124:-1 11/11 None
F46F5.4 ENSEMBL cdna:known chromosome:CEL140:II:805036:806124:-1 gene:F46F5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:805035-806124(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F46F5.4
Entrez Gene 185857 Entrez gene
SwissProt Q9TXX7 EMBL-EBI
WormBase CE19420 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC78184 Hypothetical protein F46F5.4 [Caenorhabditis elegans] ref|NP_493815.1| F46F5.4 [Caenorhabditis elegans] 1.0E-170
blast AAC78184 Hypothetical protein F46F5.4 [Caenorhabditis elegans] ref|NP_493815.1| F46F5.4 [Caenorhabditis elegans] 1.0E-118
blast AAC78192 Hypothetical protein F46F5.14 [Caenorhabditis elegans] ref|NP_493813.2| F46F5.14 [Caenorhabditis elegans] 1.0E-116
blast AAC19260 Hypothetical protein ZK1055.2 [Caenorhabditis elegans] ref|NP_504676.1| ZK1055.2 [Caenorhabditis elegans] 1.0E-108
Protein Domains
Database ID Description E-Value
Pfam IPR004988 EMBL-EBI Protein of unknown function DUF273 1.0E-126
Pfam IPR004988 EMBL-EBI Protein of unknown function DUF273 1.0E-126
Pfam IPR004988 EMBL-EBI Protein of unknown function DUF273 8.5E-29
Sequence
Target Sequence 
>C. ELEGANS:183938_AT
tgggaaattgcgtctggcttttacctcgcaaggaatacaccatttgcaattaatttatta
aacaattttgctgactatgagtctaagcttccaaacagttttcacggaacagacaatgga
gcaattcatatattcttggctgaaaaaatatttccacaagcttccatagaaatcgggatt
tgtagaagagcgtacgaaaaatcaacaagtgtcgaagatctcattttgtatgaagcatgt
atcaaggcattgtttggagttgtaacagattttggaaaaattcaaattttaagaaaagga
accggttgggcaagagatggatggttgacgtcaatggtctggcacccggagacggatttt
atgcttcatggatggaaaaccaatgcgttgataccaacgccaactgatccattagtagca
attgaaatgagtatgagccaatggttcaaccctttgaatggatcaattgatttatcaaaa
tgcaattctcacaattcaacttggaattacgacgcacgtcttcttgggaata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGGAAATTGCGTCTGGCTTTTACC 214 555 250 Antisense
GGCTTTTACCTCGCAAGGAATACAC 197 537 265 Antisense
GAGTCTAAGCTTCCAAACAGTTTTC 636 399 328 Antisense
GGAGCAATTCATATATTCTTGGCTG 679 519 367 Antisense
AAATATTTCCACAAGCTTCCATAGA 162 115 395 Antisense
AAGTGTCGAAGATCTCATTTTGTAT 41 159 456 Antisense
GGTTGACGTCAATGGTCTGGCACCC 65 507 572 Antisense
ACCCGGAGACGGATTTTATGCTTCA 474 89 593 Antisense
TACCAACGCCAACTGATCCATTAGT 105 651 641 Antisense
GTATGAGCCAATGGTTCAACCCTTT 597 455 680 Antisense
TACGACGCACGTCTTCTTGGGAATA 670 641 757 Antisense
Sequence Source Affymetrix Proprietary Database