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[183923_at]

Description T27B7.7 /REP_DB=WormBase Gene ID /WP=CE14243 /TR=O16666 /GB=AAB66139.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 45      4grp_total > 02      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 3.5
(-2.6)
A 		2.2
(-3)
A 		4.9
(-2.3)
A 		24.1
(3)
A 		22
B 6.9
(-2.3)
A 		5.1
(-2.8)
A 		4.6
(-3)
A 		23.4
(3)
A 		19
C 4.3
(-3)
A 		4.8
(-3)
A 		5.9
(-2.9)
A 		72.2
(3)
A 		68
avr 4.9
(-2.8) 		4
(-3) 		5.1
(-2.8) 		39.9
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 34.6
(2.9)
A 		2
(-3)
A 		35.4
(3)
A 		2
(-3)
A 		5.3
(-2.4)
A 		20.1
(0.3)
A 		33
B 14.7
(3)
A 		9.3
(0.4)
A 		5.4
(-1.5)
A 		2.3
(-3)
A 		3.6
(-2.4)
A 		2.6
(-2.9)
A 		12
C 5.8
(-1.7)
A 		13.2
(1.1)
A 		3
(-2.8)
A 		9.4
(-0.4)
A 		2.4
(-3)
A 		18.3
(3)
A 		16
avr 18.4
(3) 		8.2
(-1.2) 		14.6
(1.4) 		4.6
(-2.7) 		3.8
(-3) 		13.7
(1.1) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 1.3
(-1.4)
A 		0.5
(-3)
A 		1.3
(-1.4)
A 		3.5
(3)
A 		3
B 2.2
(-1.3)
A 		1.3
(-2.7)
A 		1.1
(-3)
A 		5
(3)
A 		4
C 1.4
(-3)
A 		1.4
(-3)
A 		2
(-2.7)
A 		12.2
(3)
A 		11
avr 1.6
(-2.5) 		1.1
(-3) 		1.5
(-2.6) 		6.9
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 6.4
(1.9)
A 		0.7
(-2.8)
A 		7.7
(3)
A 		0.5
(-3)
A 		1.6
(-2.1)
A 		4.9
(0.7)
A 		7
B 5.1
(3)
A 		3.1
(0.3)
A 		1.3
(-2.1)
A 		0.6
(-3)
A 		1.4
(-1.9)
A 		1
(-2.5)
A 		5
C 2.2
(-1.4)
A 		4
(0.6)
A 		0.8
(-3)
A 		1.9
(-1.8)
A 		0.8
(-3)
A 		6.1
(3)
A 		5
avr 4.6
(3) 		2.6
(-0.3) 		3.3
(0.8) 		1
(-3) 		1.3
(-2.5) 		4
(2) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183923_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15417
Sequence Type Exemplar sequence
Representative Public ID T27B7.7 NCBI
Target Description T27B7.7 /REP_DB=WormBase Gene ID /WP=CE14243 /TR=O16666 /GB=AAB66139.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071224(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071224 NCBI Caenorhabditis elegans T27B7.7 (T27B7.7) mRNA, complete cds. 11/11 None
SNAP00000042361 ENSEMBL cdna:SNAP chromosome:CEL140:V:2278654:2281215:1 11/11 None
GENEFINDER00000042366 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2278654:2281425:1 9/11 None
T27B7.7 ENSEMBL cdna:known chromosome:CEL140:V:2279270:2281215:1 gene:T27B7.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2279269-2281215(+) 91.71 100.0
Public Domain and Genome References
Gene Title
Ensembl T27B7.7
Entrez Gene 188978 Entrez gene
SwissProt O16666 EMBL-EBI
WormBase CE14243 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66139 Hypothetical protein T27B7.7 [Caenorhabditis elegans] ref|NP_503625.1| T27B7.7 [Caenorhabditis elegans] 0.0
blast AAB66139 Hypothetical protein T27B7.7 [Caenorhabditis elegans] ref|NP_503625.1| T27B7.7 [Caenorhabditis elegans] 1.0E-167
blast AAB66139 Hypothetical protein T27B7.7 [Caenorhabditis elegans] ref|NP_503625.1| T27B7.7 [Caenorhabditis elegans] 1.0E-125
blast CAE58141 Hypothetical protein CBG01230 [Caenorhabditis briggsae] 3.0E-29
blast CAE58141 Hypothetical protein CBG01230 [Caenorhabditis briggsae] 3.0E-24
blast CAE58141 Hypothetical protein CBG01230 [Caenorhabditis briggsae] 3.0E-15
blast CAB05473 Hypothetical protein D1025.3 [Caenorhabditis elegans] ref|NP_510413.1| D1025.3 [Caenorhabditis elegans] 4.0E-14
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.3E-7
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.3E-7
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.3E-7
Sequence
Target Sequence 
>C. ELEGANS:183923_AT
tgtgtacttattggagctgctcagctattttgatacttctgtgttgcggattataaaaat
caagagttattacatgctaagagacgcagtagaccgactgatagccactaagcaattcag
gaatgccaaagaagttcacatttgcccaatgatgcgtattccgattgagccatttcttca
tctggactcttttgaaatttctatgacgtctgtgaagtctgatgatgtagtcacattaat
gaagaaatttcaacaaaagcttactcccaacaactgccactttagacttacaaatgaaga
aaaacttgaagacgacttccttaacgatgtattccaaaaacttggcatagcgggcgatgg
acctgagagagaatctataagcaccgacgaaaagaatcatatctctaagtttccgaccgt
cgaagaaaaatcttggctgttggtcaaatttgaggagtactcagtattcggtgtaattgt
ttccagcgattttctggcaagtcttaaatacgacgttctcattttcatgactct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTGTACTTATTGGAGCTGCTCAGC 581 555 504 Antisense
GTAGACCGACTGATAGCCACTAAGC 662 453 592 Antisense
GATGCGTATTCCGATTGAGCCATTT 311 415 654 Antisense
TGAGCCATTTCTTCATCTGGACTCT 626 569 669 Antisense
ACAAAAGCTTACTCCCAACAACTGC 349 109 756 Antisense
CAACAACTGCCACTTTAGACTTACA 411 189 771 Antisense
GACGACTTCCTTAACGATGTATTCC 90 379 814 Antisense
TTGGCATAGCGGGCGATGGACCTGA 218 709 845 Antisense
ATCATATCTCTAAGTTTCCGACCGT 120 29 899 Antisense
GGTGTAATTGTTTCCAGCGATTTTC 629 499 973 Antisense
ACGACGTTCTCATTTTCATGACTCT 414 93 1013 Antisense
Sequence Source Affymetrix Proprietary Database