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[183921_at]

Description F26F4.2 /REP_DB=WormBase Gene ID /WP=CE01259 /TR=Q19823 /GB=AAA91217.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 88      4grp_total > 24      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 27.3
(-3)
A 		27.6
(-2.9)
A 		32.1
(-1.4)
A 		44.8
(3)
A 		18
B 16.6
(-2.2)
A 		14
(-3)
A 		33.4
(3)
A 		22.8
(-0.3)
A 		19
C 6.6
(-3)
A 		25.7
(0.7)
A 		29.4
(1.5)
A 		37.2
(3)
A 		31
avr 16.8
(-3) 		22.4
(-1.1) 		31.6
(1.9) 		34.9
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 452
1 2 3 4 5 6 range
A 57.3
(-2.4)
A 		16.2
(-2.9)
A 		11.6
(-3)
A 		53.2
(-2.4)
A 		218.6
(-0.2)
P 		459.9
(3)
P 		448
B 13.1
(-3)
A 		19.5
(-2.9)
A 		31.6
(-2.7)
A 		21
(-2.9)
A 		193.9
(-0.1)
P 		385.9
(3)
P 		373
C 31.8
(-2.6)
A 		64.3
(-2.1)
A 		8.1
(-3)
A 		66.1
(-2.1)
A 		248.8
(0.7)
P 		402.6
(3)
P 		395
avr 34.1
(-2.7) 		33.3
(-2.8) 		17.1
(-3) 		46.8
(-2.6) 		220.4
(0.1) 		416.1
(3) 		399
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 9.9
(3)
A 		6
(-3)
A 		8.4
(0.7)
A 		6.6
(-2.1)
A 		4
B 5.3
(-0.4)
A 		3.5
(-3)
A 		7.7
(3)
A 		4.8
(-1.1)
A 		4
C 2.2
(-3)
A 		7.4
(0.9)
A 		10.2
(3)
A 		6.3
(0.1)
A 		8
avr 5.8
(-2.6) 		5.6
(-3) 		8.8
(3) 		5.9
(-2.4) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 10.6
(-2.6)
A 		5.8
(-2.8)
A 		2.5
(-3)
A 		13.5
(-2.4)
A 		66
(0.5)
P 		112
(3)
P 		110
B 4.6
(-3)
A 		6.5
(-2.9)
A 		7.7
(-2.9)
A 		5.6
(-3)
A 		78.8
(0.2)
P 		143.9
(3)
P 		139
C 11.8
(-2.6)
A 		19.6
(-2.2)
A 		2.1
(-3)
A 		13.3
(-2.5)
A 		80.5
(0.5)
P 		134.8
(3)
P 		133
avr 9
(-2.8) 		10.6
(-2.7) 		4.1
(-3) 		10.8
(-2.7) 		75.1
(0.4) 		130.2
(3) 		126
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183921_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6994
Sequence Type Exemplar sequence
Representative Public ID F26F4.2 NCBI
Target Description F26F4.2 /REP_DB=WormBase Gene ID /WP=CE01259 /TR=Q19823 /GB=AAA91217.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065631(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065631 NCBI Caenorhabditis elegans F26F4.2 (F26F4.2) mRNA, complete cds. 11/11 None
SNAP00000005756 ENSEMBL cdna:SNAP chromosome:CEL140:III:4911222:4911909:1 11/11 None
F26F4.2 ENSEMBL cdna:known chromosome:CEL140:III:4911222:4911953:1 gene:F26F4.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000005769 3/11 Cross Hyb Matching Probes None
GENEFINDER00000005772 8/11 Cross Hyb Matching Probes None
F26F4.9b 11/11 Negative Strand Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4911221-4911909(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F26F4.2
Entrez Gene 184988 Entrez gene
SwissProt Q19823 EMBL-EBI
WormBase CE29288 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA91217 Spt transcription factor family protein 2 [Caenorhabditis elegans] ref|NP_498032.1| F26F4.2 [Caenorhabditis elegans] 1.0E-72
blast CAA84662 Hypothetical protein F37A8.1 [Caenorhabditis elegans] ref|NP_497793.1| F37A8.1 [Caenorhabditis elegans] 2.0E-48
blast CAE71297 Hypothetical protein CBG18186 [Caenorhabditis briggsae] 2.0E-47
Sequence
Target Sequence 
>C. ELEGANS:183921_AT
gggaccgctgtgaagtgcaagtgaccaaaccgttgaatggaaccatcggacgtagtactg
gggatgtcattgcttcaaagagcatgcagatccatgatgagccaagaccattgtctctgt
caccgcctctgcagaaatctgagaaatcttcgaagagcaagaaactttccgagtacagtt
gcatgagttcgactaaggaacaaatggagactgaaattcgcaacggttccaatgcttctg
cggcaaagaaactccaatgcaaggtcttgactgatcaagttcgtgtctcgccaatcgtct
cgtgcaacggagtgattttggagttggatgttgaagtcacaattcctgatcgtgaggaga
agaagaaattgaagttggagatcgagtgccccgattttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGACCGCTGTGAAGTGCAAGTGAC 23 495 110 Antisense
GGAACCATCGGACGTAGTACTGGGG 106 523 148 Antisense
GTACTGGGGATGTCATTGCTTCAAA 708 457 164 Antisense
GCAGATCCATGATGAGCCAAGACCA 650 311 195 Antisense
TCACCGCCTCTGCAGAAATCTGAGA 156 627 229 Antisense
GAAACTTTCCGAGTACAGTTGCATG 68 357 270 Antisense
TTCGCAACGGTTCCAATGCTTCTGC 417 695 326 Antisense
GAAACTCCAATGCAAGGTCTTGACT 429 357 357 Antisense
CAATCGTCTCGTGCAACGGAGTGAT 109 181 401 Antisense
GATGTTGAAGTCACAATTCCTGATC 471 409 436 Antisense
TTGGAGATCGAGTGCCCCGATTTTG 655 697 484 Antisense
Sequence Source Affymetrix Proprietary Database