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[183909_at]

Description W08F4.3 /REP_DB=WormBase Gene ID /WP=CE14716 /TR=O02109 /GB=AAC71167.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 46      6grp_high1_10_10 > 64      4grp_highest_8_8 > 19      4grp_high_8_8 > 63      6grp_highest_nonorm > 72      6grp_total > 53      4grp_total > 56      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 482
1 2 3 4 range
A 354.7
(-3)
P 		498.2
(-1.2)
P 		836.5
(3)
P 		511.3
(-1)
P 		482
B 588.4
(-0.8)
P 		490.4
(-3)
P 		755.4
(3)
P 		570.8
(-1.2)
P 		265
C 466.9
(-1.8)
P 		544.3
(1.7)
P 		573.5
(3)
P 		440.8
(-3)
P 		133
avr 470
(-3) 		511
(-2) 		721.8
(3) 		507.6
(-2.1) 		252

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 313
1 2 3 4 5 6 range
A 474.8
(-0.3)
P 		498
(0.3)
P 		436.8
(-1.3)
P 		511.4
(0.6)
P 		373.4
(-3)
P 		600.4
(3)
P 		227
B 404.9
(-1.5)
P 		355.7
(-3)
P 		548.6
(3)
P 		404
(-1.5)
P 		368.5
(-2.6)
P 		432.2
(-0.6)
P 		193
C 493.3
(0.4)
P 		352.8
(-2.3)
P 		623
(2.9)
P 		346.1
(-2.4)
P 		628.9
(3)
P 		315.5
(-3)
P 		313
avr 457.7
(-0.5) 		402.2
(-3) 		536.1
(3) 		420.5
(-2.2) 		456.9
(-0.5) 		449.4
(-0.9) 		134
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 144
1 2 3 4 range
A 129.2
(-0.7)
P 		108.1
(-1.6)
P 		218.7
(3)
P 		74.8
(-3)
P 		144
B 187.6
(3)
P 		121.1
(-3)
P 		175.1
(1.9)
P 		121.6
(-3)
P 		67
C 154.5
(0.8)
P 		156.5
(0.9)
P 		199.9
(3)
P 		74.3
(-3)
P 		126
avr 157.1
(0.7) 		128.6
(-0.9) 		197.9
(3) 		90.2
(-3) 		108

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 87.5
(-3)
P 		177.1
(3)
P 		95.1
(-2.5)
P 		129.7
(-0.2)
P 		112.7
(-1.3)
P 		146.2
(0.9)
P 		90
B 140.9
(0.8)
P 		118.4
(-1.7)
P 		133.2
(-0.1)
P 		107
(-3)
P 		149.7
(1.7)
P 		161.2
(3)
P 		54
C 182.9
(2.1)
P 		107.7
(-1.3)
P 		158.6
(1)
P 		69.6
(-3)
P 		203.5
(3)
P 		105.6
(-1.4)
P 		134
avr 137.1
(0.9) 		134.4
(0.6) 		129
(0) 		102.1
(-3) 		155.3
(3) 		137.7
(1) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183909_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5668
Sequence Type Exemplar sequence
Representative Public ID W08F4.3 NCBI
Target Description W08F4.3 /REP_DB=WormBase Gene ID /WP=CE14716 /TR=O02109 /GB=AAC71167.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061361(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061361 NCBI Caenorhabditis elegans W08F4.3 (W08F4.3) mRNA, complete cds. 11/11 None
SNAP00000029466 ENSEMBL cdna:SNAP chromosome:CEL140:II:580619:581834:1 11/11 None
GENEFINDER00000029472 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:580619:581834:1 11/11 None
W08F4.3.2 ENSEMBL cdna:known chromosome:CEL140:II:580598:581947:1 gene:W08F4.3 11/11 None
W08F4.3.1 ENSEMBL cdna:known chromosome:CEL140:II:580600:581999:1 gene:W08F4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:580618-581834(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W08F4.3
Entrez Gene 173450 Entrez gene
SwissProt O02109 EMBL-EBI
WormBase CE14716 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6696 ergosterol biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5783 endoplasmic reticulum inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
247 C-8 sterol isomerase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC71167 Hypothetical protein W08F4.3 [Caenorhabditis elegans] ref|NP_493762.1| W08F4.3 [Caenorhabditis elegans] 1.0E-120
blast CAE62900 Hypothetical protein CBG07090 [Caenorhabditis briggsae] 6.0E-97
Protein Domains
Database ID Description E-Value
Pfam IPR006716 EMBL-EBI ERG2 and sigma1 receptor-like protein 4.0E-113
Sequence
Target Sequence 
>C. ELEGANS:183909_AT
ggcctccgctggaagtcttatgacatctcgccgaagcagttcaagactatcgctggaaag
gctgctgctgctccaacaatctcccagtcaatttccacattgacgtctggaatgcgcact
tattacgagccaatcatgccaagtcacttcgaatggcacgctattcagttgggaggcctc
actttaaggatgtacccaatgctgaccgctttcaccgagttcgccgccgccttctccgct
ccgttcccaacttcaggacgcccggggctccattgggcaaatacgacgtgcaccgtgttg
aaaggaaaagtcagccgattcaatgacggaactcaggacgagacgtcggagaactttgct
tcgggagccgctttcaggcacggacagttcgagtcccacgtgtacagcttcgagaaggac
acctacgtcgcgtgctacggtcgcggcttcctcccactctcctccaccgtccacgtcacc
tctggaatctcactcggcgagccactctcggtcattcaattctacat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCCTCCGCTGGAAGTCTTATGACA 372 543 82 Antisense
TATCGCTGGAAAGGCTGCTGCTGCT 624 657 129 Antisense
AATCTCCCAGTCAATTTCCACATTG 23 171 159 Antisense
GACGTCTGGAATGCGCACTTATTAC 48 379 183 Antisense
TACGAGCCAATCATGCCAAGTCACT 10 649 205 Antisense
AAGTCACTTCGAATGGCACGCTATT 634 157 222 Antisense
ACGCTATTCAGTTGGGAGGCCTCAC 41 91 239 Antisense
TAAGGATGTACCCAATGCTGACCGC 286 637 266 Antisense
GTCGGAGAACTTTGCTTCGGGAGCC 344 471 426 Antisense
CGCTTTCAGGCACGGACAGTTCGAG 500 253 450 Antisense
CACTCTCGGTCATTCAATTCTACAT 77 199 584 Antisense
Sequence Source Affymetrix Proprietary Database