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[183900_at]

Description T12B3.2 /REP_DB=WormBase Gene ID /WP=CE21177 /TR=Q9UAW9 /GB=AAD14736.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_highest_nonorm > 80      6grp_total > 25      4grp_total > 29      6grp_highest_sub > 01_02      6grp_high1_sub > 01_02      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 174
1 2 3 4 range
A 365.2
(1.2)
P 		301.9
(-1)
P 		242.8
(-3)
P 		416.8
(3)
P 		174
B 360.4
(2.7)
P 		324.3
(-1.6)
P 		313.1
(-3)
P 		362.5
(3)
P 		49
C 397.5
(2.9)
P 		383.4
(2.3)
P 		253.8
(-3)
P 		399.4
(3)
P 		146
avr 374.4
(2.1) 		336.5
(0.2) 		269.9
(-3) 		392.9
(3) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 304
1 2 3 4 5 6 range
A 262.3
(1.3)
P 		310.7
(3)
P 		176.4
(-1.7)
P 		152.7
(-2.5)
P 		143.5
(-2.8)
P 		138.1
(-3)
P 		173
B 363.7
(2.1)
P 		404.8
(3)
P 		258.8
(-0.1)
P 		148.6
(-2.5)
P 		206.7
(-1.3)
P 		126.6
(-3)
P 		278
C 289.6
(1.4)
P 		359.1
(3)
P 		117
(-2.6)
P 		103.9
(-2.9)
P 		101
(-3)
P 		163.4
(-1.5)
P 		258
avr 305.2
(1.6) 		358.2
(3) 		184.1
(-1.7) 		135.1
(-3) 		150.4
(-2.6) 		142.7
(-2.8) 		223
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 133
(3)
P 		65.5
(-2.6)
P 		63.5
(-2.8)
P 		61
(-3)
P 		72
B 114.9
(3)
P 		80.1
(-1.9)
P 		72.6
(-3)
P 		77.2
(-2.3)
P 		42
C 131.6
(3)
P 		110.2
(1)
P 		88.4
(-1)
P 		67.3
(-3)
P 		64
avr 126.5
(3) 		85.3
(-1.3) 		74.8
(-2.3) 		68.5
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 48.4
(-1.8)
P 		110.5
(3)
P 		38.4
(-2.6)
P 		38.7
(-2.6)
P 		43.3
(-2.2)
P 		33.6
(-3)
P 		77
B 126.6
(2.5)
P 		134.7
(3)
P 		62.8
(-1.5)
P 		39.4
(-3)
P 		84
(-0.2)
P 		47.2
(-2.5)
P 		95
C 107.3
(2.8)
P 		109.6
(3)
P 		29.8
(-2.4)
P 		20.9
(-3)
P 		32.7
(-2.2)
P 		54.7
(-0.7)
P 		89
avr 94.1
(1.3) 		118.3
(3) 		43.7
(-2.2) 		33
(-3) 		53.3
(-1.6) 		45.2
(-2.1) 		85
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183900_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9081
Sequence Type Exemplar sequence
Representative Public ID T12B3.2 NCBI
Target Description T12B3.2 /REP_DB=WormBase Gene ID /WP=CE21177 /TR=Q9UAW9 /GB=AAD14736.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068776(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068776 NCBI Caenorhabditis elegans T12B3.2 (T12B3.2) mRNA, complete cds. 11/11 None
SNAP00000023939 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7376889:7378815:1 9/11 None
GENEFINDER00000023943 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7376889:7378815:1 11/11 None
T12B3.2 ENSEMBL cdna:known chromosome:CEL140:IV:7376889:7378867:1 gene:T12B3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7376894-7378821(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T12B3.2
Entrez Gene 188439 Entrez gene
SwissProt Q9UAW9 EMBL-EBI
WormBase CE21177 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAD14736 Hypothetical protein T12B3.2 [Caenorhabditis elegans] ref|NP_501177.1| T12B3.2 [Caenorhabditis elegans] 0.0
blast CAE61870 Hypothetical protein CBG05850 [Caenorhabditis briggsae] 0.0
blast CAA93635 Hypothetical protein C44C10.3 [Caenorhabditis elegans] ref|NP_509958.2| C44C10.3 [Caenorhabditis elegans] 1.0E-177
Protein Domains
Database ID Description E-Value
Pfam IPR005828 EMBL-EBI General substrate transporter 4.7E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501177.1 11 7-29,88-107,114-136,140-162,175-192,202-224,275-294,309-331,359-381,396-418,425-447
T12B3.2 11 7-29,88-107,114-136,140-162,175-192,202-224,275-294,309-331,359-381,396-418,425-447
SNAP00000023939 6 7-29,70-92,105-122,132-154,205-227,260-282
GENEFINDER00000023943 11 7-29,88-107,114-136,140-162,175-192,202-224,275-294,309-331,359-381,396-418,425-447
Sequence
Target Sequence 
>C. ELEGANS:183900_AT
aaacgatttaagctccggcactttggcgggcgatttgtatctcaatcagctgttctttgg
aattttacttgttttttccaaaatgttactgctatttgtagacacatattttacaaattt
caagagaagaacattacatcaaggatctcaaataggaacactttgctgttttatcattct
tactacttttatgagtatggattatcatggaatcggagttcttatcgtctatctgattgg
aacaacatttatcgaatacacatgggatgcctgttacctttgtgctatagaactcatgga
gacaccttcaagagcaagtgcaactggatcatgttctcttattgcaagaattggaatgat
tctagcaccattcctaacatatgcaaatacttggtggtctcctgctgttaatgttactgt
aattgtattgggaagtgctaatttactgatttcatatttctttttgcccgaatccaaagg
agtgaatctcgacgatgtacacgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACGATTTAAGCTCCGGCACTTTG 213 23 885 Antisense
GGCACTTTGGCGGGCGATTTGTATC 189 535 901 Antisense
GCTGTTTTATCATTCTTACTACTTT 465 293 1049 Antisense
GAATCGGAGTTCTTATCGTCTATCT 333 329 1094 Antisense
ATGCCTGTTACCTTTGTGCTATAGA 572 41 1151 Antisense
ACTGGATCATGTTCTCTTATTGCAA 510 97 1207 Antisense
ATGATTCTAGCACCATTCCTAACAT 304 47 1240 Antisense
GCAAATACTTGGTGGTCTCCTGCTG 258 317 1267 Antisense
GGTCTCCTGCTGTTAATGTTACTGT 325 503 1280 Antisense
CATATTTCTTTTTGCCCGAATCCAA 138 215 1337 Antisense
GAGTGAATCTCGACGATGTACACGT 513 401 1364 Antisense
Sequence Source Affymetrix Proprietary Database