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[183898_at]

Description R74.7 /REP_DB=WormBase Gene ID /WP=CE23932 /TR=SW:Q22031 /GB=CAA85279.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 54      6grp_high1_10_10 > 34      4grp_highest_8_8 > 25      4grp_high_8_8 > 60      6grp_highest_nonorm > 73      6grp_total > 0      4grp_total > 60      6grp_total_sub > Hat      6grp_high1_sub > 02      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 486
1 2 3 4 range
A 491.2
(-2.6)
P 		596.2
(2)
P 		620.1
(3)
P 		483.2
(-3)
P 		137
B 650.8
(2.6)
P 		547.8
(1.3)
P 		686.5
(3)
P 		200.2
(-3)
P 		486
C 619.4
(1.8)
P 		638.9
(2.4)
P 		661.9
(3)
P 		445.2
(-3)
P 		217
avr 587.1
(1.5) 		594.3
(1.7) 		656.2
(3) 		376.2
(-3) 		280

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 368
1 2 3 4 5 6 range
A 485.5
(-2.2)
P 		519.5
(-0.4)
P 		586.4
(3)
P 		469.5
(-3)
P 		488.8
(-2)
P 		496.7
(-1.6)
P 		117
B 592.7
(-1.2)
P 		669.1
(0.1)
P 		488.1
(-3)
P 		837.5
(3)
P 		669
(0.1)
P 		528.5
(-2.3)
P 		349
C 513.7
(-2.3)
P 		792.2
(3)
P 		479.4
(-3)
P 		486.4
(-2.9)
P 		537.9
(-1.9)
P 		722.1
(1.7)
P 		313
avr 530.6
(-2.5) 		660.3
(3) 		518
(-3) 		597.8
(0.4) 		565.2
(-1) 		582.4
(-0.3) 		142
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 188
1 2 3 4 range
A 178.8
(3)
P 		129.4
(0.3)
P 		162.1
(2.1)
P 		70.7
(-3)
P 		108
B 207.5
(3)
P 		135.3
(0.4)
P 		159.1
(1.2)
P 		42.7
(-3)
P 		165
C 205
(2)
P 		183.7
(1.2)
P 		230.7
(3)
P 		75.1
(-3)
P 		156
avr 197.1
(3) 		149.5
(0.9) 		184
(2.4) 		62.8
(-3) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 182
1 2 3 4 5 6 range
A 89.5
(-3)
P 		184.8
(3)
P 		127.7
(-0.6)
P 		119.1
(-1.1)
P 		147.5
(0.7)
P 		120.9
(-1)
P 		95
B 206.2
(0.4)
P 		222.7
(1.1)
P 		118.5
(-3)
P 		221.8
(1)
P 		271.8
(3)
P 		197.1
(0.1)
P 		153
C 190.4
(0.9)
P 		241.7
(3)
P 		122
(-2)
P 		97.8
(-3)
P 		174.1
(0.2)
P 		241.7
(3)
P 		144
avr 162
(-0.5) 		216.4
(3) 		122.7
(-3) 		146.2
(-1.5) 		197.8
(1.8) 		186.6
(1.1) 		94
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183898_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7547
Sequence Type Exemplar sequence
Representative Public ID R74.7 NCBI
Target Description R74.7 /REP_DB=WormBase Gene ID /WP=CE23932 /TR=SW:Q22031 /GB=CAA85279.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065442(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065442 NCBI Caenorhabditis elegans R74.7 (R74.7) mRNA, complete cds. 11/11 None
SNAP00000044752 ENSEMBL cdna:SNAP chromosome:CEL140:III:4204409:4205649:1 11/11 None
GENEFINDER00000044754 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4204409:4205649:1 11/11 None
R74.7 ENSEMBL cdna:known chromosome:CEL140:III:4204400:4205728:1 gene:R74.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4204408-4205649(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R74.7
Entrez Gene 175543 Entrez gene
SwissProt Q22031 EMBL-EBI
WormBase CE23932 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251139_AT FtsJ-like methyltransferase family protein at
CANINE:1605513_S_AT similar to FtsJ homolog 1 isoform b cfa
CANINE_2:CFA.19997.1.S1_AT similar to FtsJ homolog 1 isoform b cfa
CANINE_2:CFA.19997.1.S1_S_AT similar to FtsJ homolog 1 isoform b cfa
CANINE_2:CFAAFFX.23581.1.S1_AT similar to FtsJ homolog 1 isoform b cfa
DROSOPHILA_2:1626096_AT dm
DROSGENOME1:154071_AT dm
HG-U133_PLUS_2:205324_S_AT FtsJ homolog 1 (E. coli) hs
HG-U133A:205324_S_AT FtsJ homolog 1 (E. coli) hs
HG-FOCUS:205324_S_AT FtsJ homolog 1 (E. coli) hs
HG-U133A_2:205324_S_AT FtsJ homolog 1 (E. coli) hs
HU35KSUBA:RC_AA399418_AT FtsJ homolog 1 (E. coli) hs
HU35KSUBD:RC_N94524_AT FtsJ homolog 1 (E. coli) hs
U133_X3P:G7110660_3P_A_AT FtsJ homolog 1 (E. coli) hs
U133_X3P:HS.23170.2.S1_3P_A_AT FtsJ homolog 1 (E. coli) hs
HG-U133A_2:213937_S_AT FtsJ homolog 1 (E. coli) hs
HG-U133_PLUS_2:213937_S_AT FtsJ homolog 1 (E. coli) hs
HG-U133A:213937_S_AT FtsJ homolog 1 (E. coli) hs
HG-U95AV2:33437_AT FtsJ homolog 1 (E. coli) hs
U133_X3P:HS.23170.2.S1_3P_X_AT FtsJ homolog 1 (E. coli) hs
U133_X3P:HS.23170.2.S1_3P_AT FtsJ homolog 1 (E. coli) hs
MG-U74BV2:106059_AT Ftsj homolog 1 (E. coli) mm
MOE430A:1417099_AT Ftsj homolog 1 (E. coli) mm
MOUSE430A_2:1417099_AT Ftsj homolog 1 (E. coli) mm
MOUSE430_2:1417099_AT Ftsj homolog 1 (E. coli) mm
MOE430B:1430381_AT Ftsj homolog 1 (E. coli) mm
MOUSE430_2:1430381_AT Ftsj homolog 1 (E. coli) mm
MU19KSUBB:TC25977_AT Ftsj homolog 1 (E. coli), mRNA (cDNA clone MGC:41313 IMAGE:3468633) mm
MU19KSUBB:TC26948_AT Ftsj homolog 1 (E. coli), mRNA (cDNA clone MGC:41313 IMAGE:3468633) mm
RAE230B:1393914_AT similar to Ftsj homolog rn
RAT230_2:1393914_AT similar to Ftsj homolog rn
YEAST_2:1776697_AT 2'-O-ribose methyltransferase, methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop Sc
Protein Similarities
Method ID Description E-Value
blast CAA85279 Hypothetical protein R74.7 [Caenorhabditis elegans] ref|NP_497843.1| R74.7 [Caenorhabditis elegans] sp|Q22031|RRM2_CAEEL Putative ribosomal RNA methyltransferase R74.7 (rRNA (uridine-2'-O-)-methyltransferase) 0.0
blast B88422 protein R74.7 [imported] - Caenorhabditis elegans 1.0E-173
blast CAE72863 Hypothetical protein CBG20162 [Caenorhabditis briggsae] 1.0E-157
Protein Domains
Database ID Description E-Value
Pfam IPR002877 EMBL-EBI Ribosomal RNA methyltransferase RrmJ/FtsJ 5.4E-91
Sequence
Target Sequence 
>C. ELEGANS:183898_AT
atgcaagccgaactaatcctcgctgccttcaacatcacaagtcacgttctcaaagaaggc
ggcaactttctcgcgaaaattttccgatcccgaaactcctctcttctctatgcacaaatg
aagaagtattttaaaaaagtatatctagccaaaccacgaagttcccgtcaatccagttgt
gaagcttttgtcctgtgtctcgactattcaccgcccgagggatttgttccaacaatggga
aagacttcattagacgcaactgatgcttctgctatttcacctgatattatcgatggattc
gtgacttgtggcgatctaagtggatgggacagcgagaagtcttatccgttggatattgat
gcttgttttcccaaaggagagattgatgaagagcagaaaaaacgatacgagttcaaagac
gtcgttcagccaccaacagatcctgcctacaaagcagctctcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCAAGCCGAACTAATCCTCGCTG 198 43 406 Antisense
CTGCCTTCAACATCACAAGTCACGT 285 237 428 Antisense
CAAAGAAGGCGGCAACTTTCTCGCG 42 189 456 Antisense
TCTCGCGAAAATTTTCCGATCCCGA 156 607 474 Antisense
TCCTCTCTTCTCTATGCACAAATGA 332 593 502 Antisense
TCTAGCCAAACCACGAAGTTCCCGT 131 611 549 Antisense
GTTCCCGTCAATCCAGTTGTGAAGC 87 441 566 Antisense
TTTGTCCTGTGTCTCGACTATTCAC 705 665 592 Antisense
TTCACCGCCCGAGGGATTTGTTCCA 199 685 612 Antisense
GTTCAAAGACGTCGTTCAGCCACCA 570 443 816 Antisense
GATCCTGCCTACAAAGCAGCTCTCG 514 415 844 Antisense
Sequence Source Affymetrix Proprietary Database