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[183895_at]

Description Y43F8C.1 /REP_DB=WormBase Gene ID /WP=CE21899 /TR=Q9XWP3 /GB=CAA21608.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 04      6grp_highest_nonorm > 40      6grp_total > 15      4grp_total > 51      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 460
1 2 3 4 range
A 242.7
(0.5)
P 		398.7
(3)
P 		256.2
(0.7)
P 		28.9
(-3)
A 		370
B 175
(-0.2)
P 		347
(3)
P 		221.6
(0.7)
P 		22.9
(-3)
A 		324
C 294.8
(0.7)
P 		473
(3)
P 		254.7
(0.2)
P 		13.2
(-3)
A 		460
avr 237.5
(0.4) 		406.2
(3) 		244.2
(0.5) 		21.7
(-3) 		385

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1357
1 2 3 4 5 6 range
A 623.6
(0.5)
P 		1001.2
(3)
P 		765.6
(1.5)
P 		295.7
(-1.6)
P 		191.6
(-2.3)
P 		80.2
(-3)
P 		921
B 983.9
(2.9)
P 		998.3
(3)
P 		519.7
(-0.2)
P 		192.3
(-2.5)
P 		270.6
(-1.9)
P 		111.9
(-3)
P 		886
C 1437.4
(3)
P 		795.5
(0.1)
P 		378.4
(-1.8)
P 		254.1
(-2.3)
P 		282.8
(-2.2)
P 		100.3
(-3)
P 		1337
avr 1015
(3) 		931.7
(2.5) 		554.6
(-0) 		247.4
(-2) 		248.3
(-2) 		97.5
(-3) 		918
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 134
1 2 3 4 range
A 88.4
(3)
P 		86.5
(2.9)
P 		67
(1.5)
P 		4.2
(-3)
A 		84
B 55.8
(0.8)
P 		85.7
(3)
P 		51.4
(0.5)
P 		4.9
(-3)
A 		81
C 97.6
(1.3)
P 		136
(3)
P 		88.8
(0.9)
P 		2.2
(-3)
A 		134
avr 80.6
(1.7) 		102.7
(3) 		69.1
(1) 		3.8
(-3) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 513
1 2 3 4 5 6 range
A 115
(-1.3)
P 		356.1
(3)
P 		166.7
(-0.4)
P 		75
(-2)
P 		57.8
(-2.3)
P 		19.5
(-3)
P 		337
B 342.3
(3)
P 		332.3
(2.8)
P 		126.2
(-1.3)
P 		50.9
(-2.8)
P 		110
(-1.6)
P 		41.7
(-3)
P 		301
C 532.8
(3)
P 		242.7
(-0.5)
P 		96.3
(-2.2)
P 		51.1
(-2.8)
P 		91.5
(-2.3)
P 		33.6
(-3)
P 		499
avr 330
(3) 		310.4
(2.6) 		129.7
(-1) 		59
(-2.4) 		86.4
(-1.9) 		31.6
(-3) 		298
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183895_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12868
Sequence Type Exemplar sequence
Representative Public ID Y43F8C.1 NCBI
Target Description Y43F8C.1 /REP_DB=WormBase Gene ID /WP=CE21899 /TR=Q9XWP3 /GB=CAA21608.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075400(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075400 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-25) (nlp-25) mRNA, complete cds. 11/11 None
SNAP00000039606 ENSEMBL cdna:SNAP chromosome:CEL140:V:19610083:19610918:1 11/11 None
GENEFINDER00000039627 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19610083:19610918:1 11/11 None
Y43F8C.1 ENSEMBL cdna:known chromosome:CEL140:V:19610083:19610918:1 gene:Y43F8C.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19610082-19610918(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-25
Ensembl Y43F8C.1
Entrez Gene 189877 Entrez gene
SwissProt Q9XWP3 EMBL-EBI
WormBase CE21899 Wormbase
Sequence
Target Sequence 
>C. ELEGANS:183895_AT
gcaatcactaattgcgcttctcctgctattagtgtgcttcttacacttggcacagtgcca
atggggtggtggatggaataatggaggtggatatggtaacccgtatgggggttatggcag
aggtggaggatatggtggcggatatggaggtggttttggagcacagcaagcctataatgt
gcagaatgcagctagaattggaacagaagttgcagaaggtgtcttggtagct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAATCACTAATTGCGCTTCTCCTG 175 323 15 Antisense
CTTCTCCTGCTATTAGTGTGCTTCT 180 221 31 Antisense
GTGTGCTTCTTACACTTGGCACAGT 510 487 46 Antisense
GGCACAGTGCCAATGGGGTGGTGGA 532 535 63 Antisense
GGGGTGGTGGATGGAATAATGGAGG 687 497 77 Antisense
GGAGGTGGATATGGTAACCCGTATG 76 495 97 Antisense
CCCGTATGGGGGTTATGGCAGAGGT 487 255 114 Antisense
GTGGTTTTGGAGCACAGCAAGCCTA 53 489 164 Antisense
CAAGCCTATAATGTGCAGAATGCAG 310 185 181 Antisense
GCAGAATGCAGCTAGAATTGGAACA 265 311 195 Antisense
AGTTGCAGAAGGTGTCTTGGTAGCT 344 65 222 Antisense
Sequence Source Affymetrix Proprietary Database