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[183888_at]

Description W03A5.7 /REP_DB=WormBase Gene ID /WP=CE21253 /TR=Q9TY07 /GB=AAD31932.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 36      4grp_total > 33      6grp_cgc > 05      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 56.9
(-3)
A 		114.8
(3)
A 		70.4
(-1.6)
A 		105.2
(2)
A 		58
B 55.4
(-3)
A 		114.6
(1.5)
A 		120.9
(1.9)
M 		135.1
(3)
P 		80
C 54.6
(-3)
A 		118
(3)
A 		112.2
(2.5)
P 		94.8
(0.8)
A 		63
avr 55.6
(-3) 		115.8
(3) 		101.2
(1.5) 		111.7
(2.6) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 106
1 2 3 4 5 6 range
A 74.3
(3)
A 		10
(-2.4)
A 		69.1
(2.6)
A 		3
(-3)
A 		54.8
(1.4)
A 		73
(2.9)
A 		71
B 92
(1.3)
M 		108.7
(3)
A 		54
(-2.5)
A 		92.2
(1.3)
A 		74.6
(-0.4)
A 		49
(-3)
A 		60
C 36.1
(-3)
A 		108.9
(3)
A 		57.1
(-1.3)
A 		45.1
(-2.3)
A 		60.2
(-1)
A 		87.9
(1.3)
A 		73
avr 67.5
(1.3) 		75.9
(3) 		60.1
(-0.3) 		46.8
(-3) 		63.2
(0.4) 		70
(1.8) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 20.7
(0.3)
A 		24.9
(3)
A 		18.4
(-1.1)
A 		15.4
(-3)
A 		10
B 17.7
(-3)
A 		28.3
(2.7)
A 		28
(2.6)
M 		28.8
(3)
P 		11
C 18.1
(-2.5)
A 		33.9
(1.6)
A 		39.1
(3)
P 		16
(-3)
A 		23
avr 18.8
(-3) 		29
(3) 		28.5
(2.7) 		20.1
(-2.2) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 13.7
(1.6)
A 		3.6
(-2)
A 		15.1
(2)
A 		0.8
(-3)
A 		16.5
(2.5)
A 		17.8
(3)
A 		17
B 32
(1.9)
M 		36.2
(3)
A 		13.1
(-3)
A 		24.4
(-0.1)
A 		30.3
(1.5)
A 		18.3
(-1.6)
A 		23
C 13.4
(-1.9)
A 		33.2
(3)
A 		14.5
(-1.7)
A 		9.1
(-3)
A 		19.5
(-0.4)
A 		29.4
(2.1)
A 		24
avr 19.7
(0.9) 		24.3
(3) 		14.2
(-1.7) 		11.4
(-3) 		22.1
(2) 		21.8
(1.8) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183888_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6452
Sequence Type Exemplar sequence
Representative Public ID W03A5.7 NCBI
Target Description W03A5.7 /REP_DB=WormBase Gene ID /WP=CE21253 /TR=Q9TY07 /GB=AAD31932.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065754(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065754 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-24) (dnj-24) mRNA, complete cds. 11/11 None
SNAP00000014241 ENSEMBL cdna:SNAP chromosome:CEL140:III:5416091:5417462:-1 9/11 None
GENEFINDER00000014245 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5416091:5419521:-1 11/11 None
W03A5.7 ENSEMBL cdna:known chromosome:CEL140:III:5416766:5419521:-1 gene:W03A5.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000014242 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5416764-5420339(-) 97.18 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dnj-24
Ensembl W03A5.7
Entrez Gene 175745 Entrez gene
SwissProt Q9TY07 EMBL-EBI
WormBase CE38983 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1598571_S_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 6 isoform a cfa
CANINE_2:CFA.3079.1.S1_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 6 isoform a cfa
CANINE_2:CFAAFFX.8707.1.S1_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 6 isoform a cfa
CANINE_2:CFAAFFX.8707.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 6 isoform a cfa
DROSOPHILA_2:1638963_S_AT dm
DROSGENOME1:153707_AT dm
CHICKEN:GGA.9160.1.S1_AT DnaJ (Hsp40) homolog, subfamily B, member 6 gga
CHICKEN:GGA.9160.2.S1_A_AT DnaJ (Hsp40) homolog, subfamily B, member 6 gga
CHICKEN:GGA.9160.3.S1_AT DnaJ (Hsp40) homolog, subfamily B, member 6 gga
HG-U133A:208810_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-U133_PLUS_2:208810_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-U133A_2:208810_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-FOCUS:208811_S_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-U133_PLUS_2:208811_S_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-U133A_2:208811_S_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-U133A:208811_S_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-U133_PLUS_2:209015_S_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-U133A_2:209015_S_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-U133A:209015_S_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HU35KSUBA:RC_AA084898_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HU35KSUBA:AA496105_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
U133_X3P:G4322314_3P_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
U133_X3P:G4322314_3P_A_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
U133_X3P:G12803262_3P_S_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
U133_X3P:208810_3P_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-U95AV2:41233_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-U95AV2:41234_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-U95E:73008_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
HG-U95D:74442_AT DnaJ (Hsp40) homolog, subfamily B, member 6 hs
RG-U34C:RC_AI179560_AT DnaJ (Hsp40) homolog, subfamily B, member 6 (predicted) rn
RAE230B:1382024_AT DnaJ (Hsp40) homolog, subfamily B, member 6 (predicted) rn
RAT230_2:1382024_AT DnaJ (Hsp40) homolog, subfamily B, member 6 (predicted) rn
RAE230B:1391422_AT DnaJ (Hsp40) homolog, subfamily B, member 6 (predicted) rn
RAT230_2:1391422_AT DnaJ (Hsp40) homolog, subfamily B, member 6 (predicted) rn
RAE230B:1378843_AT DnaJ (Hsp40) homolog, subfamily B, member 6 (predicted) rn
RAT230_2:1378843_AT DnaJ (Hsp40) homolog, subfamily B, member 6 (predicted) rn
RAE230B:1392598_AT DnaJ (Hsp40) homolog, subfamily B, member 6 (predicted) rn
RAT230_2:1392598_AT DnaJ (Hsp40) homolog, subfamily B, member 6 (predicted) rn
RG-U34C:RC_AI231687_AT DnaJ (Hsp40) homolog, subfamily B, member 6 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast NP_498155 DNaJ domain (prokaryotic heat shock protein) family member (dnj-24) [Caenorhabditis elegans] 1.0E-139
blast AAD31932 Dnaj domain (prokaryotic heat shock protein) protein 24 [Caenorhabditis elegans] 1.0E-136
blast AAD31932 Dnaj domain (prokaryotic heat shock protein) protein 24 [Caenorhabditis elegans] 1.0E-130
blast NP_498155 DNaJ domain (prokaryotic heat shock protein) family member (dnj-24) [Caenorhabditis elegans] 1.0E-130
blast CAE61337 Hypothetical protein CBG05175 [Caenorhabditis briggsae] 1.0E-128
blast CAE61337 Hypothetical protein CBG05175 [Caenorhabditis briggsae] 1.0E-122
blast AAD31932 Dnaj domain (prokaryotic heat shock protein) protein 24 [Caenorhabditis elegans] 2.0E-81
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 3.99999987306209E-21
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 9.99999968265523E-22
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.9E-31
Sequence
Target Sequence 
>C. ELEGANS:183888_AT
taaaatggcaccccgacaagcatacagacgacaaatcaaaagaagaagccgaacaaaagt
tcaagaaaattgcacaagcttacgagattttaacagataaaaaaaagcgcgccgacctgg
accgaaccgaaaatccagggctccaccgtcgccgcagtacccctggaggcatgcaccgaa
tgcacagtcacgatatgttccgatcaccttttgatatttttcgcgagttcttcggcaacc
gggatccatttgagaacgtgtttttcgacgacgcgttcacatttcccgacgtcgattcgt
acgcgttcaagcatccaccacggtccgctgatttcacaacgaagaatcattatcatcgat
tcccgtcaagtcgagtgcacattttttacgacgagaacaagaacaccaaggagagagacg
acaaaaacacattttcgactgtaatccgattctcaagtcccacggaacctggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAAATGGCACCCCGACAAGCATAC 6 641 272 Antisense
GGCATGCACCGAATGCACAGTCACG 301 537 439 Antisense
AGTCACGATATGTTCCGATCACCTT 566 59 457 Antisense
TCACCTTTTGATATTTTTCGCGAGT 226 625 475 Antisense
TTCGGCAACCGGGATCCATTTGAGA 430 693 502 Antisense
GAGAACGTGTTTTTCGACGACGCGT 504 359 523 Antisense
CGACGTCGATTCGTACGCGTTCAAG 672 249 558 Antisense
CGGTCCGCTGATTTCACAACGAAGA 542 243 592 Antisense
CATTATCATCGATTCCCGTCAAGTC 574 215 619 Antisense
GTCAAGTCGAGTGCACATTTTTTAC 273 463 636 Antisense
TTCTCAAGTCCCACGGAACCTGGAA 306 671 721 Antisense
Sequence Source Affymetrix Proprietary Database