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[183867_at]

Description C45E5.4 /REP_DB=WormBase Gene ID /WP=CE08732 /TR=Q18642 /GB=AAC24289.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 21      4grp_total > 09      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 380
1 2 3 4 range
A 79.2
(-1.9)
A 		29.6
(-3)
A 		123.8
(-0.9)
A 		298.7
(3)
M 		269
B 65.6
(-2.2)
A 		124.8
(-1.1)
A 		24.7
(-3)
A 		345.5
(3)
P 		321
C 46.6
(-3)
A 		87.5
(-2.3)
A 		118.4
(-1.8)
A 		404.4
(3)
P 		358
avr 63.8
(-3) 		80.6
(-2.6) 		89
(-2.5) 		349.5
(3) 		286

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 272
1 2 3 4 5 6 range
A 161.7
(0.5)
A 		176.4
(1)
A 		243.4
(3)
A 		145.7
(0.1)
A 		98.5
(-1.4)
A 		43.8
(-3)
A 		200
B 117.7
(-1.4)
A 		292
(3)
M 		190.9
(0.4)
A 		116.1
(-1.5)
A 		151.7
(-0.6)
A 		55.7
(-3)
A 		236
C 95.2
(-1.3)
A 		286.9
(3)
A 		121.5
(-0.7)
A 		117.4
(-0.8)
A 		92.4
(-1.4)
A 		20.3
(-3)
A 		267
avr 124.9
(-0.6) 		251.8
(3) 		185.3
(1.1) 		126.4
(-0.6) 		114.2
(-0.9) 		39.9
(-3) 		212
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 28.8
(0.6)
A 		6.4
(-3)
A 		32.4
(1.2)
A 		43.7
(3)
M 		37
B 20.9
(-1.7)
A 		30.8
(-0.8)
A 		5.7
(-3)
A 		73.6
(3)
P 		68
C 15.4
(-3)
A 		25.1
(-1.9)
A 		41.3
(-0.1)
A 		68.2
(3)
P 		53
avr 21.7
(-2.9) 		20.8
(-3) 		26.5
(-2.2) 		61.8
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 29.8
(-0.8)
A 		62.7
(3)
A 		53
(1.9)
A 		37
(0)
A 		29.7
(-0.8)
A 		10.7
(-3)
A 		52
B 40.9
(-1.4)
A 		97.2
(3)
M 		46.4
(-1)
A 		30.7
(-2.2)
A 		61.6
(0.2)
A 		20.8
(-3)
A 		76
C 35.3
(-0.9)
A 		87.5
(3)
A 		30.9
(-1.2)
A 		23.6
(-1.8)
A 		29.9
(-1.3)
A 		6.8
(-3)
A 		81
avr 35.3
(-1.1) 		82.5
(3) 		43.4
(-0.4) 		30.4
(-1.5) 		40.4
(-0.6) 		12.8
(-3) 		70
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183867_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10458
Sequence Type Exemplar sequence
Representative Public ID C45E5.4 NCBI
Target Description C45E5.4 /REP_DB=WormBase Gene ID /WP=CE08732 /TR=Q18642 /GB=AAC24289.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068453(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068453 NCBI Caenorhabditis elegans C45E5.4 (C45E5.4) mRNA, complete cds. 11/11 None
SNAP00000012951 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5735046:5736906:1 11/11 None
GENEFINDER00000012957 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5735046:5736906:1 11/11 None
C45E5.4 ENSEMBL cdna:known chromosome:CEL140:IV:5735046:5736906:1 gene:C45E5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5735051-5736912(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C45E5.4
Entrez Gene 183472 Entrez gene
SwissProt Q18642 EMBL-EBI
WormBase CE08732 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC24289 Hypothetical protein C45E5.4 [Caenorhabditis elegans] ref|NP_500854.1| C45E5.4 [Caenorhabditis elegans] 1.0E-89
blast AAC24289 Hypothetical protein C45E5.4 [Caenorhabditis elegans] ref|NP_500854.1| C45E5.4 [Caenorhabditis elegans] 1.0E-83
blast CAE72660 Hypothetical protein CBG19873 [Caenorhabditis briggsae] 5.0E-66
blast CAE72660 Hypothetical protein CBG19873 [Caenorhabditis briggsae] 2.0E-61
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000012957 1 141-163
Sequence
Target Sequence 
>C. ELEGANS:183867_AT
gattcttggcttcttgtactttgattttttcgattactctggtttcggcgagatcatctc
ggttcacgaggcaggccgatgagactctcaccgactccgaaggatctggtcttcaattta
taaatttcgatgagggatcagcatcaaatccaataattgcaagagatcaggaaggatccg
gacagatgtatgacgaagaaggttctggaaatactcttctttttgattctgaaactccaa
ttcaagtgatgaatgttactggaacagaagccgaacctacagattcaaaagttttcaatc
aatcaattaatatcaccgatagtgacgtggaaaccctggtgatcaaagtgcacttgccgc
aaaaaactacgagccagctgctcgacgatctcaaagacgatactgctcagcagacgaacc
caatcaacacattggacacagccgtctctctgaccagtatgctcaacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCTTGGCTTCTTGTACTTTGAT 27 431 20 Antisense
GATTACTCTGGTTTCGGCGAGATCA 621 429 51 Antisense
GCGAGATCATCTCGGTTCACGAGGC 56 291 67 Antisense
CACGAGGCAGGCCGATGAGACTCTC 493 199 84 Antisense
CTCACCGACTCCGAAGGATCTGGTC 235 229 106 Antisense
GGAAATACTCTTCTTTTTGATTCTG 515 527 226 Antisense
GGAACAGAAGCCGAACCTACAGATT 48 529 280 Antisense
TAGTGACGTGGAAACCCTGGTGATC 5 653 339 Antisense
ACTACGAGCCAGCTGCTCGACGATC 507 75 385 Antisense
TACTGCTCAGCAGACGAACCCAATC 610 645 420 Antisense
GTCTCTCTGACCAGTATGCTCAACG 340 469 463 Antisense
Sequence Source Affymetrix Proprietary Database