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[183820_at]

Description F32B5.4 /REP_DB=WormBase Gene ID /WP=CE09852 /TR=O01851 /GB=AAB54208.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 26      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 3.8
(-3)
A 		7.6
(-0.2)
A 		10
(1.7)
A 		11.8
(3)
A 		8
B 7.8
(0.3)
A 		10.5
(3)
A 		4.5
(-3)
A 		9.2
(1.7)
A 		6
C 6.2
(-1)
A 		8.6
(1.5)
A 		4.2
(-3)
A 		10.1
(3)
A 		6
avr 5.9
(-3) 		8.9
(1) 		6.2
(-2.6) 		10.4
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 225
1 2 3 4 5 6 range
A 12.2
(-2.8)
A 		3.2
(-3)
A 		8.1
(-2.9)
A 		9.4
(-2.8)
A 		206.5
(2.4)
A 		228.5
(3)
P 		225
B 8.7
(-2.9)
A 		3.7
(-3)
A 		8.2
(-2.9)
A 		10.3
(-2.8)
A 		209.9
(3)
A 		192.7
(2.5)
P 		206
C 7.8
(-2.9)
A 		8.4
(-2.9)
A 		4.4
(-3)
A 		18.5
(-2.6)
A 		225.1
(2.9)
P 		227.4
(3)
P 		223
avr 9.6
(-2.9) 		5.1
(-3) 		6.9
(-2.9) 		12.7
(-2.8) 		213.8
(2.9) 		216.2
(3) 		211
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 1.4
(-3)
A 		1.7
(-1.5)
A 		2.6
(3)
A 		1.7
(-1.5)
A 		1
B 2.5
(2.6)
A 		2.6
(3)
A 		1
(-3)
A 		2
(0.8)
A 		2
C 2
(0)
A 		2.5
(3)
A 		1.5
(-3)
A 		1.7
(-1.8)
A 		1
avr 2
(0) 		2.3
(3) 		1.7
(-3) 		1.8
(-2) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 2.2
(-2.9)
A 		1.1
(-3)
A 		1.8
(-2.9)
A 		2.4
(-2.9)
A 		62.3
(3)
A 		55.6
(2.3)
P 		61
B 3
(-2.9)
A 		1.2
(-3)
A 		2
(-2.9)
A 		2.7
(-2.9)
A 		85.3
(3)
A 		71.9
(2)
P 		84
C 2.9
(-2.9)
A 		2.6
(-2.9)
A 		1.1
(-3)
A 		3.7
(-2.8)
A 		72.8
(2.7)
P 		76.1
(3)
P 		75
avr 2.7
(-2.9) 		1.6
(-3) 		1.6
(-3) 		2.9
(-2.9) 		73.5
(3) 		67.9
(2.5) 		72
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183820_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.61
Sequence Type Exemplar sequence
Representative Public ID F32B5.4 NCBI
Target Description F32B5.4 /REP_DB=WormBase Gene ID /WP=CE09852 /TR=O01851 /GB=AAB54208.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058623(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058623 NCBI Caenorhabditis elegans F32B5.4 (F32B5.4) mRNA, complete cds. 11/11 None
SNAP00000012560 ENSEMBL cdna:SNAP chromosome:CEL140:I:2692110:2692400:1 11/11 None
F32B5.4 ENSEMBL cdna:known chromosome:CEL140:I:2692110:2692400:1 gene:F32B5.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000012569 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2692109-2692400(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F32B5.4
Entrez Gene 171830 Entrez gene
SwissProt O01851 EMBL-EBI
WormBase CE09852 Wormbase
Functional Annotations
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491024.1 2 7-24,78-95
F32B5.4 2 7-24,78-95
SNAP00000012560 2 7-24,78-95
Sequence
Target Sequence 
>C. ELEGANS:183820_AT
gatccgtcaaacaactacagttctggttgtgttgtcccttctcgccatgtttgtggctca
ggaagtcgccgaggaagctcttacggatccagaagcagctgaagcggataatgccaaaaa
caatgcaacggatgctcccgctgatgctacaccgggatctggatcagatgctccagctgc
tccagaaggatctggcgccgaagccgaagccaccacagcgaagagttctactgctgcagt
gaccatcattgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCCGTCAAACAACTACAGTTCTG 89 417 15 Antisense
CTACAGTTCTGGTTGTGTTGTCCCT 112 227 29 Antisense
CCTTCTCGCCATGTTTGTGGCTCAG 640 269 51 Antisense
TTGTGGCTCAGGAAGTCGCCGAGGA 277 709 65 Antisense
GTCGCCGAGGAAGCTCTTACGGATC 537 475 79 Antisense
GCTCTTACGGATCCAGAAGCAGCTG 31 301 91 Antisense
AAACAATGCAACGGATGCTCCCGCT 656 127 132 Antisense
CTGATGCTACACCGGGATCTGGATC 460 239 155 Antisense
CCAGAAGGATCTGGCGCCGAAGCCG 622 271 196 Antisense
AAGCCACCACAGCGAAGAGTTCTAC 95 149 221 Antisense
CTACTGCTGCAGTGACCATCATTGG 153 227 242 Antisense
Sequence Source Affymetrix Proprietary Database