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[183795_s_at]

Description T25D3.2 /REP_DB=WormBase Gene ID /WP=CE19599 /TR=O76606 /GB=AAC26291.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 55      6grp_high1_10_10 > 54      4grp_highest_8_8 > 18      4grp_high_8_8 > 63      6grp_highest_nonorm > 73      6grp_total > 55      4grp_total > 56      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 174
1 2 3 4 range
A 682.7
(-3)
P 		683.6
(-2.9)
P 		706.8
(-0.7)
P 		745.8
(3)
P 		63
B 639.1
(0.2)
P 		664.9
(2)
P 		678.7
(3)
P 		595
(-3)
P 		84
C 687.9
(0.1)
P 		649.1
(-1.2)
P 		768.5
(3)
P 		599.9
(-3)
P 		169
avr 669.9
(-1.1) 		665.9
(-1.4) 		718
(3) 		646.9
(-3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 384
1 2 3 4 5 6 range
A 439.5
(-3)
P 		512.7
(-1.9)
P 		823.3
(3)
P 		621.7
(-0.2)
P 		478.2
(-2.4)
P 		612.3
(-0.3)
P 		384
B 651.6
(0.6)
P 		685.7
(1.8)
P 		549.9
(-3)
P 		590.1
(-1.6)
P 		563.3
(-2.5)
P 		720.1
(3)
P 		170
C 508.1
(-1.7)
P 		689.9
(3)
P 		544.5
(-0.8)
P 		612.8
(1)
P 		458
(-3)
P 		625.3
(1.3)
P 		232
avr 533.1
(-1.7) 		629.4
(2.1) 		639.2
(2.5) 		608.2
(1.3) 		499.8
(-3) 		652.6
(3) 		153
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 167
1 2 3 4 range
A 248.6
(3)
P 		148.4
(-1.3)
P 		184.8
(0.3)
P 		109.2
(-3)
P 		139
B 203.8
(3)
P 		164.2
(-0.1)
P 		157.3
(-0.6)
P 		126.8
(-3)
P 		77
C 227.7
(1.6)
P 		186.6
(0.1)
P 		267.9
(3)
P 		101.2
(-3)
P 		167
avr 226.7
(3) 		166.4
(-0.2) 		203.3
(1.8) 		112.4
(-3) 		114

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 188
1 2 3 4 5 6 range
A 81
(-3)
P 		182.3
(3)
P 		179.3
(2.8)
P 		157.7
(1.5)
P 		144.3
(0.7)
P 		149.1
(1)
P 		101
B 226.7
(1.1)
P 		228.3
(1.2)
P 		133.5
(-3)
P 		156.3
(-2)
P 		228.9
(1.2)
P 		268.5
(3)
P 		135
C 188.3
(1.5)
P 		210.5
(3)
P 		138.6
(-1.9)
P 		123.2
(-3)
P 		148.2
(-1.3)
P 		209.3
(2.9)
P 		87
avr 165.3
(-1.1) 		207
(2.8) 		150.5
(-2.5) 		145.7
(-3) 		173.8
(-0.3) 		209
(3) 		63
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 183795_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5908
Sequence Type Exemplar sequence
Representative Public ID T25D3.2 NCBI
Target Description T25D3.2 /REP_DB=WormBase Gene ID /WP=CE19599 /TR=O76606 /GB=AAC26291.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061271(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061271 NCBI Caenorhabditis elegans T25D3.2 (T25D3.2) mRNA, complete cds. 11/11 None
SNAP00000025782 ENSEMBL cdna:SNAP chromosome:CEL140:II:149019:156007:-1 11/11 None
GENEFINDER00000025787 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:154935:156007:-1 11/11 None
T25D3.2 ENSEMBL cdna:known chromosome:CEL140:II:154935:156007:-1 gene:T25D3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:154934-156007(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T25D3.2
Entrez Gene 173400 Entrez gene
SwissProt O76606 EMBL-EBI
WormBase CE19599 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.16264.1.S1_A_AT similar to mitochondrial ribosomal protein L28 cfa
CANINE_2:CFA.16264.2.S1_X_AT similar to mitochondrial ribosomal protein L28 cfa
CANINE_2:CFAAFFX.30102.1.S1_S_AT similar to mitochondrial ribosomal protein L28 cfa
DROSOPHILA_2:1640523_AT mitochondrial ribosomal protein L28 dm
DROSGENOME1:145800_AT mitochondrial ribosomal protein L28 dm
HG-U95AV2:1550_AT mitochondrial ribosomal protein L28 hs
HC-G110:1550_AT mitochondrial ribosomal protein L28 hs
HG-U95AV2:1551_G_AT mitochondrial ribosomal protein L28 hs
HC-G110:1551_G_AT mitochondrial ribosomal protein L28 hs
HG-U133_PLUS_2:204599_S_AT mitochondrial ribosomal protein L28 hs
HG-U133A:204599_S_AT mitochondrial ribosomal protein L28 hs
HG-FOCUS:204599_S_AT mitochondrial ribosomal protein L28 hs
HG-U133A_2:204599_S_AT mitochondrial ribosomal protein L28 hs
HU35KSUBA:U19796_AT mitochondrial ribosomal protein L28 hs
HUGENEFL:U19796_AT mitochondrial ribosomal protein L28 hs
HU35KSUBB:RC_AA011209_S_AT mitochondrial ribosomal protein L28 hs
U133_X3P:G5453835_3P_A_AT mitochondrial ribosomal protein L28 hs
MG-U74AV2:96652_AT mitochondrial ribosomal protein L28 mm
MOE430A:1456313_X_AT mitochondrial ribosomal protein L28 mm
MOUSE430A_2:1456313_X_AT mitochondrial ribosomal protein L28 mm
MOUSE430_2:1456313_X_AT mitochondrial ribosomal protein L28 mm
MOE430A:1437622_X_AT mitochondrial ribosomal protein L28 mm
MOUSE430A_2:1437622_X_AT mitochondrial ribosomal protein L28 mm
MOUSE430_2:1437622_X_AT mitochondrial ribosomal protein L28 mm
MOE430A:1416284_AT mitochondrial ribosomal protein L28 mm
MOUSE430A_2:1416284_AT mitochondrial ribosomal protein L28 mm
MOUSE430_2:1416284_AT mitochondrial ribosomal protein L28 mm
MU19KSUBB:TC31670_AT Mitochondrial ribosomal protein L28, mRNA (cDNA clone IMAGE:3707759) mm
RG-U34C:RC_AI235502_AT similar to Mrpl28 protein rn
RAE230A:1373705_AT similar to Mrpl28 protein rn
RAT230_2:1373705_AT similar to Mrpl28 protein rn
Protein Similarities
Method ID Description E-Value
blast AAC26291 Hypothetical protein T25D3.2 [Caenorhabditis elegans] ref|NP_493672.1| T25D3.2 [Caenorhabditis elegans] 1.0E-179
blast AAC26291 Hypothetical protein T25D3.2 [Caenorhabditis elegans] ref|NP_493672.1| T25D3.2 [Caenorhabditis elegans] 1.0E-176
blast CAE62799 Hypothetical protein CBG06974 [Caenorhabditis briggsae] 1.0E-149
blast CAE62799 Hypothetical protein CBG06974 [Caenorhabditis briggsae] 1.0E-146
blast AAH95131 Hypothetical protein LOC554098 [Danio rerio] ref|NP_001019570.1| hypothetical protein LOC554098 [Danio rerio] 6.0E-36
Sequence
Target Sequence 
>C. ELEGANS:183795_S_AT
aaggatggattgagtcggcgccgtacacgaaaaagaagattctaccaagatattgggtgc
caaagttgtttttcccggctctgaagaacgtagtgttgtattcagagattttggataaat
atatcaaggtaacagtcacggaaagagcgatgagattaatcgatgaacattttggattag
actattacattcttgaaagcaaggaaatcgatttagactcgaaatttgcgaattcgctga
aacgcgaaatgctccttacattgaccaccgagtcctactacgaggaaaatgacgagaaga
aagcgtatatcaaggagaaatacgctgattttgtgattccagagagagaagcagaatgga
tcggactggaattgaatgaagcggcccgtaaacaacaggatatggaagattcaacagcac
cggagccattgaaatataaatttgaacaatctcttctcgaacgtcttcgtgatggaactg
atgagatctcccaagaactcgagaatccagaggctccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGATGGATTGAGTCGGCGCCGTA 575 159 341 Antisense
GAGTCGGCGCCGTACACGAAAAAGA 153 399 352 Antisense
TGCCAAAGTTGTTTTTCCCGGCTCT 572 585 398 Antisense
TTCCCGGCTCTGAAGAACGTAGTGT 144 699 412 Antisense
CGAAATGCTCCTTACATTGACCACC 272 253 585 Antisense
GAAGCGGCCCGTAAACAACAGGATA 324 341 718 Antisense
AAGATTCAACAGCACCGGAGCCATT 660 155 746 Antisense
TTGAACAATCTCTTCTCGAACGTCT 158 705 782 Antisense
GAACGTCTTCGTGATGGAACTGATG 531 347 799 Antisense
TGATGAGATCTCCCAAGAACTCGAG 117 567 819 Antisense
GAACTCGAGAATCCAGAGGCTCCAA 29 349 835 Antisense
Sequence Source Affymetrix Proprietary Database