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[183783_at]

Description ZK185.2 /REP_DB=WormBase Gene ID /WP=CE17311 /TR=O44196 /GB=AAB88559.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 08      4grp_total > 39      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 125
1 2 3 4 range
A 178.9
(3)
P 		149
(0.4)
A 		110.1
(-3)
A 		124.3
(-1.8)
A 		69
B 214
(3)
P 		116.4
(-3)
A 		136.6
(-1.8)
A 		151.7
(-0.8)
A 		98
C 234.8
(3)
P 		114.7
(-3)
A 		142.1
(-1.6)
A 		166.3
(-0.4)
A 		120
avr 209.2
(3) 		126.7
(-3) 		129.6
(-2.8) 		147.4
(-1.5) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 194.2
(3)
A 		136.6
(0.4)
A 		130.2
(0.2)
A 		134.5
(0.3)
A 		59.4
(-3)
A 		120.1
(-0.3)
A 		135
B 192
(3)
P 		141.8
(-0.7)
A 		174.3
(1.7)
A 		154.6
(0.2)
A 		124.9
(-2)
A 		111.3
(-3)
A 		81
C 160.7
(3)
A 		145.9
(1.9)
A 		130.7
(0.7)
A 		144.5
(1.7)
A 		83.1
(-3)
A 		97.6
(-1.9)
A 		78
avr 182.3
(3) 		141.4
(0.4) 		145.1
(0.6) 		144.5
(0.6) 		89.1
(-3) 		109.7
(-1.7) 		93
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 65.1
(3)
P 		32.3
(-1.2)
A 		28.8
(-1.6)
A 		18.2
(-3)
A 		47
B 68.2
(3)
P 		28.7
(-3)
A 		31.6
(-2.6)
A 		32.3
(-2.5)
A 		40
C 77.7
(3)
P 		33
(-2.4)
A 		49.5
(-0.4)
A 		28
(-3)
A 		50
avr 70.3
(3) 		31.3
(-2.3) 		36.6
(-1.6) 		26.2
(-3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 35.8
(0.5)
A 		48.6
(3)
A 		28.3
(-1)
A 		34.1
(0.2)
A 		17.9
(-3)
A 		29.2
(-0.8)
A 		31
B 66.8
(3)
P 		47.2
(-1.6)
A 		42.3
(-2.7)
A 		41
(-3)
A 		50.7
(-0.7)
A 		41.5
(-2.9)
A 		26
C 59.6
(3)
A 		44.5
(0.2)
A 		33.3
(-1.8)
A 		29.1
(-2.6)
A 		26.9
(-3)
A 		32.7
(-1.9)
A 		33
avr 54.1
(3) 		46.8
(1) 		34.6
(-2.2) 		34.7
(-2.2) 		31.8
(-3) 		34.5
(-2.3) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183783_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10607
Sequence Type Exemplar sequence
Representative Public ID ZK185.2 NCBI
Target Description ZK185.2 /REP_DB=WormBase Gene ID /WP=CE17311 /TR=O44196 /GB=AAB88559.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068185(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068185 NCBI Caenorhabditis elegans ZK185.2 (ZK185.2) mRNA, complete cds. 11/11 None
SNAP00000040944 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4536546:4543268:1 11/11 None
GENEFINDER00000040949 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4536546:4541671:1 11/11 None
ZK185.2 ENSEMBL cdna:known chromosome:CEL140:IV:4536546:4541766:1 gene:ZK185.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4539048-4541677(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK185.2
Entrez Gene 177221 Entrez gene
SwissProt O44196 EMBL-EBI
WormBase CE31747 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6812 cation transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8324 cation transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB88559 Hypothetical protein ZK185.2 [Caenorhabditis elegans] ref|NP_500586.2| ZK185.2 [Caenorhabditis elegans] 0.0
blast CAE58546 Hypothetical protein CBG01705 [Caenorhabditis briggsae] 0.0
blast CAE58449 Hypothetical protein CBG01586 [Caenorhabditis briggsae] 3.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR006667 EMBL-EBI MgtE integral membrane region 1.2E-47
Pfam IPR006667 EMBL-EBI MgtE integral membrane region 9.3E-5
Pfam IPR006667 EMBL-EBI MgtE integral membrane region 2.1E-6
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500586.2 9 86-108,168-190,202-224,239-261,268-290,305-322,392-414,424-446,459-481
ZK185.2 9 86-108,168-190,202-224,239-261,268-290,305-322,392-414,424-446,459-481
SNAP00000040944 9 86-108,168-190,202-224,239-261,268-290,305-322,392-414,424-446,459-481
GENEFINDER00000040949 9 86-108,168-190,202-224,239-261,268-290,305-322,392-414,424-446,459-481
Sequence
Target Sequence 
>C. ELEGANS:183783_AT
tggccaatcctttgcgcaatgatgattaccacattcagcgggcttgttctcaaaaaatct
atgcaaacctatcccccactggcagcattccagccactaattaatggtctcggcgggaat
ttggtagctgttcaggcaagcagaatatccacacaacttcacagagcacggaagaatcga
gaaatcgaagttagatcacttcaaacttacatcaatccctggagggcgttcttcgggaat
aacgaagacgcttcggctgctcaaattctacttggaatttctgttccatcaaatataatt
ttcatctttatcatctttttattcggcgttggcttccacaacaccactcctttcaccatt
tcatttgttctagtttgtgcaattcaggtaacaaccctcctgtacatctgtcaaatcctg
gtccgcgcaatgtggataatgagaatcgacccggataactctgcaattccattcctaacg
gctctcggtgacctgataggctcactacttctgatcctctgtttctggagccaatggaaa
tattttgggctcccggtgtca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGCCAATCCTTTGCGCAATGATGA 123 545 865 Antisense
TACCACATTCAGCGGGCTTGTTCTC 349 649 891 Antisense
ATATCCACACAACTTCACAGAGCAC 411 21 1009 Antisense
AAACTTACATCAATCCCTGGAGGGC 517 117 1067 Antisense
TCTTTTTATTCGGCGTTGGCTTCCA 198 615 1178 Antisense
CAGGTAACAACCCTCCTGTACATCT 2 207 1249 Antisense
TGTACATCTGTCAAATCCTGGTCCG 97 559 1265 Antisense
GAGAATCGACCCGGATAACTCTGCA 544 391 1305 Antisense
TGATAGGCTCACTACTTCTGATCCT 20 565 1358 Antisense
GATCCTCTGTTTCTGGAGCCAATGG 331 415 1377 Antisense
GAAATATTTTGGGCTCCCGGTGTCA 239 361 1401 Antisense
Sequence Source Affymetrix Proprietary Database