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[183769_s_at] Elongation factor 1

Description F54H12.6 /REP_DB=WormBase Gene ID /WP=CE00548 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Elongation factor 1
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 41      4grp_highest_8_8 > 14      4grp_high_8_8 > 14      6grp_highest_nonorm > 29      6grp_total > 52      4grp_total > 09      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14673
1 2 3 4 range
A 15220.6
(-3)
P 		17294.4
(-2)
P 		17900.9
(-1.8)
P 		28102.4
(3)
P 		12882
B 14665.9
(-3)
P 		15482.9
(-2.5)
P 		17562
(-1.2)
P 		24246.9
(3)
P 		9581
C 13616.4
(-3)
P 		15648.9
(-2.2)
P 		14832.4
(-2.5)
P 		28289.8
(3)
P 		14673
avr 14501
(-3) 		16142.1
(-2.2) 		16765.1
(-1.9) 		26879.7
(3) 		12379

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4932
1 2 3 4 5 6 range
A 12862.8
(-2.5)
P 		13005.9
(-2.2)
P 		15578
(3)
P 		14876.8
(1.6)
P 		12591.2
(-3)
P 		13793.6
(-0.6)
P 		2987
B 13115.5
(3)
P 		12677
(1.7)
P 		12620.1
(1.6)
P 		11521.3
(-1.6)
P 		12276.9
(0.6)
P 		11031.3
(-3)
P 		2084
C 12237.9
(-0.6)
P 		12933.1
(0.5)
P 		14580.7
(3)
P 		12653.5
(0.1)
P 		13282.3
(1)
P 		10646.1
(-3)
P 		3935
avr 12738.7
(-0.7) 		12872
(-0.4) 		14259.6
(3) 		13017.2
(-0.1) 		12716.8
(-0.8) 		11823.7
(-3) 		2436
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1788
1 2 3 4 range
A 5541.8
(3)
P 		3754.1
(-3)
P 		4679.7
(0.1)
P 		4113.6
(-1.8)
P 		1788
B 4676.9
(0.8)
P 		3823.9
(-3)
P 		4069.5
(-1.9)
P 		5167
(3)
P 		1343
C 4507.4
(-2.9)
P 		4499.1
(-3)
P 		5169.6
(3)
P 		4770.1
(-0.6)
P 		671
avr 4908.7
(3) 		4025.7
(-3) 		4639.6
(1.2) 		4683.6
(1.5) 		883

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2617
1 2 3 4 5 6 range
A 2371.2
(-3)
P 		4625.6
(3)
P 		3392.6
(-0.3)
P 		3773.1
(0.7)
P 		3799.5
(0.8)
P 		3358.3
(-0.4)
P 		2254
B 4563.6
(1.7)
P 		4219.6
(0.6)
P 		3064.5
(-3)
P 		3051.6
(-3)
P 		4988
(3)
P 		4113.5
(0.3)
P 		1936
C 4536.1
(3)
P 		3946.3
(1.2)
P 		3711
(0.5)
P 		2544.6
(-3)
P 		4298
(2.3)
P 		3563.9
(0.1)
P 		1992
avr 3823.6
(0.4) 		4263.8
(2.5) 		3389.4
(-1.7) 		3123.1
(-3) 		4361.8
(3) 		3678.6
(-0.3) 		1239
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 183769_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7226
Sequence Type Exemplar sequence
Representative Public ID F54H12.6 NCBI
Target Description F54H12.6 /REP_DB=WormBase Gene ID /WP=CE00548 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Elongation factor 1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066336(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066336 NCBI Caenorhabditis elegans F54H12.6 (F54H12.6) mRNA, complete cds. 11/11 None
SNAP00000022464 ENSEMBL cdna:SNAP chromosome:CEL140:III:7972388:7973194:-1 11/11 None
GENEFINDER00000022471 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7972388:7973194:-1 11/11 None
F54H12.6 ENSEMBL cdna:known chromosome:CEL140:III:7972337:7973310:-1 gene:F54H12.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7972385-7973192(-) 100.0 100.0
Public Domain and Genome References
Gene Title Elongation factor 1
Ensembl F54H12.6
Entrez Gene 176120 Entrez gene
SwissProt P34460 EMBL-EBI
WormBase CE00548 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245949_AT elongation factor 1B alpha-subunit 2 (eEF1Balpha2) at
CANINE:1587669_S_AT similar to eukaryotic translation elongation factor 1 beta 2 cfa
CANINE_2:CFA.559.1.S1_AT similar to eukaryotic translation elongation factor 1 beta 2 cfa
CANINE_2:CFA.559.1.S1_S_AT similar to eukaryotic translation elongation factor 1 beta 2 cfa
DROSOPHILA_2:1634095_AT Elongation factor 1 dm
DROSGENOME1:154264_AT Elongation factor 1 dm
CHICKEN:GGA.11318.1.S1_AT eukaryotic translation elongation factor 1 beta 2 gga
CHICKEN:GGA.4763.1.S1_S_AT Eukaryotic translation elongation factor 1 beta 2 gga
CHICKEN:GGA.4763.1.S2_AT Eukaryotic translation elongation factor 1 beta 2 gga
HG-U95AV2:35748_AT eukaryotic translation elongation factor 1 beta 2 hs
HG-U133_PLUS_2:200705_S_AT eukaryotic translation elongation factor 1 beta 2 hs
HG-U133A_2:200705_S_AT eukaryotic translation elongation factor 1 beta 2 hs
HG-U133A:200705_S_AT eukaryotic translation elongation factor 1 beta 2 hs
HG-FOCUS:200705_S_AT eukaryotic translation elongation factor 1 beta 2 hs
HUGENEFL:X60489_AT eukaryotic translation elongation factor 1 beta 2 hs
U133_X3P:G4503476_3P_S_AT eukaryotic translation elongation factor 1 beta 2 hs
HU35KSUBB:RC_R94601_AT Eukaryotic translation elongation factor 1 beta 2 hs
MU11KSUBA:C78522_RC_F_AT eukaryotic translation elongation factor 1 beta 2 mm
MU11KSUBB:MSA.5029.0_F_AT eukaryotic translation elongation factor 1 beta 2 mm
MG-U74CV2:169018_AT eukaryotic translation elongation factor 1 beta 2 mm
MOE430A:1448252_A_AT eukaryotic translation elongation factor 1 beta 2 mm
MOUSE430A_2:1448252_A_AT eukaryotic translation elongation factor 1 beta 2 mm
MOUSE430_2:1448252_A_AT eukaryotic translation elongation factor 1 beta 2 mm
MG-U74AV2:102107_AT Eukaryotic translation elongation factor 1 beta 2, mRNA (cDNA clone MGC:28092 IMAGE:3963930) mm
MG-U74CV2:141174_AT Eukaryotic translation elongation factor 1 beta 2, mRNA (cDNA clone MGC:28092 IMAGE:3963930) mm
MG-U74AV2:96624_R_AT Eukaryotic translation elongation factor 1 beta 2, mRNA (cDNA clone MGC:28092 IMAGE:3963930) mm
MU19KSUBA:TC14503_AT Eukaryotic translation elongation factor 1 beta 2, mRNA (cDNA clone MGC:28092 IMAGE:3963930) mm
MU19KSUBC:TC39826_F_AT Eukaryotic translation elongation factor 1 beta 2, mRNA (cDNA clone MGC:28092 IMAGE:3963930) mm
MU19KSUBC:TC39826_I_AT Eukaryotic translation elongation factor 1 beta 2, mRNA (cDNA clone MGC:28092 IMAGE:3963930) mm
MU19KSUBC:TC39827_F_AT Eukaryotic translation elongation factor 1 beta 2, mRNA (cDNA clone MGC:28092 IMAGE:3963930) mm
RG-U34C:RC_AI175551_AT eukaryotic translation elongation factor 1 beta 2 (predicted) rn
RAE230A:1388449_AT eukaryotic translation elongation factor 1 beta 2 (predicted) rn
RAT230_2:1388449_AT eukaryotic translation elongation factor 1 beta 2 (predicted) rn
YEAST_2:1769785_AT Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6414 translational elongation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5853 eukaryotic translation elongation factor 1 complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3746 translation elongation factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA28051 Hypothetical protein F54H12.6 [Caenorhabditis elegans] ref|NP_498737.1| F54H12.6 [Caenorhabditis elegans] sp|P34460|EF1B1_CAEEL Probable elongation factor 1-beta/1-delta 1 (EF-1-beta/delta 1) 3.0E-84
blast CAE75034 Hypothetical protein CBG22942 [Caenorhabditis briggsae] 1.0E-81
blast CAB63360 Hypothetical protein Y41E3.10 [Caenorhabditis elegans] ref|NP_502816.2| Y41E3.10 [Caenorhabditis elegans] sp|Q9U2H9|EF1B2_CAEEL Probable elongation factor 1-beta/1-delta 2 (EF-1-beta/delta 2) 6.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR001326 EMBL-EBI Elongation factor 1, beta/beta/delta chain 6.9E-48
Sequence
Target Sequence 
>C. ELEGANS:183769_S_AT
caagagcccagctggacttgctgctttcaacactactctcgccgagcaggcttttgccac
tggcttcgtcttgtctggagaggacgcccaacttttcgccgctctcggatccgccccaaa
cgcctccacctatccaaatgttgcccgttggtacgctaatgtagcttcttacactgatgc
tgagcgcaagacctgggcttccgccggaggatcagctccagctgccgctgctgctgatgg
agatgacttcgatcttttcggatccgacgatgaggaggaagatgccgagaaggcaaagat
cgttgaggagcgtctcgccgcttacgccgagaagaaggccaagaaggctggaccaattgc
taaatcttcagtcattcttgatgtaaagccatgggacgatgagaccgacctcggagagat
ggagaagctcgttcgttctatcgagatggacggacttgtctggggaggagctaagcttat
cccaattggatacggaatcaagaagctccagatcatcactgttatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGAGCCCAGCTGGACTTGCTGCT 68 185 27 Antisense
TGGCTTCGTCTTGTCTGGAGAGGAC 464 545 87 Antisense
AATGTTGCCCGTTGGTACGCTAATG 563 163 163 Antisense
GTAGCTTCTTACACTGATGCTGAGC 438 457 187 Antisense
TGATGCTGAGCGCAAGACCTGGGCT 237 567 201 Antisense
AAAGATCGTTGAGGAGCGTCTCGCC 60 123 321 Antisense
GGCTGGACCAATTGCTAAATCTTCA 555 539 372 Antisense
AGCCATGGGACGATGAGACCGACCT 507 81 413 Antisense
TGGAGAAGCTCGTTCGTTCTATCGA 255 549 446 Antisense
GAGGAGCTAAGCTTATCCCAATTGG 449 403 491 Antisense
GAAGCTCCAGATCATCACTGTTATC 301 343 528 Antisense
Sequence Source Affymetrix Proprietary Database