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[183764_at]

Description Y6B3B.10 /REP_DB=WormBase Gene ID /WP=CE20184 /TR=Q9XWE9 /GB=CAA21723.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 26      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 60      6grp_total > 25      4grp_total > 44      6grp_highest_sub > De      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 277
1 2 3 4 range
A 450.4
(3)
P 		438.9
(2.5)
P 		347.8
(-1.2)
P 		304.7
(-3)
P 		146
B 433.8
(1.9)
P 		462.7
(3)
P 		334.1
(-2)
P 		308.8
(-3)
P 		154
C 418.4
(0.9)
P 		513.6
(3)
P 		356.4
(-0.4)
P 		236.9
(-3)
P 		277
avr 434.2
(1.8) 		471.7
(3) 		346.1
(-1) 		283.5
(-3) 		188

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 481
1 2 3 4 5 6 range
A 636.4
(3)
P 		447.7
(0.6)
P 		360.5
(-0.6)
P 		171.5
(-3)
P 		211
(-2.5)
P 		232.8
(-2.2)
P 		465
B 652.5
(3)
P 		572.2
(1.9)
P 		347.9
(-1.1)
P 		277.7
(-2)
P 		202.8
(-3)
P 		274.3
(-2)
P 		450
C 572.7
(3)
P 		534.3
(2.4)
P 		358.8
(-0.2)
P 		172.9
(-3)
P 		224.4
(-2.2)
P 		250.2
(-1.8)
P 		400
avr 620.5
(3) 		518.1
(1.5) 		355.7
(-0.8) 		207.4
(-3) 		212.7
(-2.9) 		252.4
(-2.3) 		413
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 124
1 2 3 4 range
A 164
(3)
P 		95.3
(-0.5)
P 		90.9
(-0.7)
P 		44.6
(-3)
P 		119
B 138.3
(3)
P 		114.3
(1)
P 		77.4
(-2)
P 		65.8
(-3)
P 		73
C 138.5
(2.5)
P 		147.7
(3)
P 		124.2
(1.7)
P 		39.9
(-3)
P 		108
avr 146.9
(3) 		119.1
(1.3) 		97.5
(-0.1) 		50.1
(-3) 		97

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 192
1 2 3 4 5 6 range
A 117.3
(0.8)
P 		159.2
(3)
P 		78.5
(-1.2)
P 		43.5
(-3)
P 		63.7
(-2)
P 		56.7
(-2.3)
P 		116
B 227.1
(3)
P 		190.5
(1.6)
P 		84.5
(-2.6)
P 		73.5
(-3)
P 		82.4
(-2.7)
P 		102.3
(-1.9)
P 		154
C 212.3
(3)
P 		163
(1.3)
P 		91.3
(-1.1)
P 		34.8
(-3)
P 		72.6
(-1.7)
P 		83.8
(-1.3)
P 		178
avr 185.6
(3) 		170.9
(2.3) 		84.8
(-1.5) 		50.6
(-3) 		72.9
(-2) 		80.9
(-1.7) 		135
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183764_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1081
Sequence Type Exemplar sequence
Representative Public ID Y6B3B.10 NCBI
Target Description Y6B3B.10 /REP_DB=WormBase Gene ID /WP=CE20184 /TR=Q9XWE9 /GB=CAA21723.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061002(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061002 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-160) (tag-160) mRNA, complete cds. 11/11 None
GENEFINDER00000043757 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13732789:13740108:-1 11/11 None
Y6B3B.10 ENSEMBL cdna:known chromosome:CEL140:I:13732789:13740108:-1 gene:Y6B3B.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043748 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13732719-13740039(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-160
Ensembl Y6B3B.10
Entrez Gene 189370 Entrez gene
SwissProt Q9XWE9 EMBL-EBI
WormBase CE20184 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGAAFFX.1997.1.S1_AT similar to longevity assurance gene 1 isoform 1; upstream of gdf1; upstream of growth and differentiation factor 1; longevity assurance (LAG1, S. cerevisiae) homolog 1 gga
HG-U95AV2:887_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HC-G110:887_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HG-U133_PLUS_2:206397_X_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HG-U133A:206397_X_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HG-FOCUS:206397_X_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HG-U133A_2:206397_X_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HU35KSUBA:M62302_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HUGENEFL:M62302_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HU35KSUBB:RC_R56800_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HG-U95AV2:888_S_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HC-G110:888_S_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
U133_X3P:G11641419_3P_S_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
U133_X3P:HS.92614.1.S1_3P_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HG-U133_PLUS_2:229448_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HG-U133B:229448_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HG-U95B:50096_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
HG-U95C:63708_AT LAG1 longevity assurance homolog 1 (S. cerevisiae) hs
MU11KSUBB:MSA.690.0_AT longevity assurance homolog 1 (S. cerevisiae) mm
MG-U74AV2:93642_AT longevity assurance homolog 1 (S. cerevisiae) mm
MOE430A:1416900_S_AT longevity assurance homolog 1 (S. cerevisiae) mm
MOUSE430A_2:1416900_S_AT longevity assurance homolog 1 (S. cerevisiae) mm
MOUSE430_2:1416900_S_AT longevity assurance homolog 1 (S. cerevisiae) mm
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA21723 Hypothetical protein Y6B3B.10 [Caenorhabditis elegans] ref|NP_493403.1| Temporarily Assigned Gene name family member (tag-160) [Caenorhabditis elegans] 0.0
blast CAE68020 Hypothetical protein CBG13635 [Caenorhabditis briggsae] 0.0
blast NP_619588 longevity assurance homolog 1 [Mus musculus] sp|P27545|LASS1_MOUSE LAG1 longevity assurance homolog 1 (UOG-1 protein) gb|AAA37675.1| ORF 2.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR005547 EMBL-EBI Longevity-assurance protein (LAG1) 3.7E-106
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493403.1 5 66-83,158-177,192-214,246-268,288-310
Y6B3B.10 5 66-83,158-177,192-214,246-268,288-310
GENEFINDER00000043757 5 66-83,158-177,192-214,246-268,288-310
Sequence
Target Sequence 
>C. ELEGANS:183764_AT
tatgcagttcaatcggcgttctatattcattcaatttatgccacattgtttatggattta
tggagaaaggattcatggctaatgtttgtacaccattttattgcacttggattacttttt
ctgtcttatgttgataatttcaccctccccggcgccctggtcctcttcctacacgacaat
tcagatgctacactggaaatcaccaaactatcgttttatttgaaaaagcgaacaaatcgt
caatattacaaatattatttcctaatgggaaatgctgcattcatactatttgctatcatt
tgggtgattttccgtttgtactggtacacttgtaaattgttatatgctacaatttatgga
gcagtgtacttgggaccacaggatgctccgttcttcccgttgctcggtgcaatgcttttg
atcatttttgcgatgaatgtttattggtttaattttatcgctcgaatgatctggcgcgta
gccttaaccggagaggatccagaagacaatcgtgaatgggacacaaccgctgtcagtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGCAGTTCAATCGGCGTTCTATA 662 655 490 Antisense
GTTTGTACACCATTTTATTGCACTT 490 451 573 Antisense
GCACTTGGATTACTTTTTCTGTCTT 492 315 592 Antisense
TTATGTTGATAATTTCACCCTCCCC 178 681 615 Antisense
TCCTCTTCCTACACGACAATTCAGA 290 593 650 Antisense
GTGATTTTCCGTTTGTACTGGTACA 34 485 793 Antisense
GTACTTGGGACCACAGGATGCTCCG 465 459 855 Antisense
CCCGTTGCTCGGTGCAATGCTTTTG 173 261 885 Antisense
TTATCGCTCGAATGATCTGGCGCGT 202 681 944 Antisense
TCTGGCGCGTAGCCTTAACCGGAGA 286 601 959 Antisense
GAATGGGACACAACCGCTGTCAGTG 346 333 1003 Antisense
Sequence Source Affymetrix Proprietary Database