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[183730_at]

Description F53E2.1 /REP_DB=WormBase Gene ID /WP=CE20847 /TR=Q9UAU3 /GB=AAD14702.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 06      4grp_high_8_8 > 16      6grp_highest_nonorm > 71      6grp_total > 06      4grp_total > 31      6grp_highest_sub > De      6grp_cgc > 21      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 336
1 2 3 4 range
A 507.6
(3)
P 		316.7
(-3)
M 		332.9
(-2.5)
P 		399.7
(-0.4)
P 		191
B 459.4
(3)
P 		340
(-1.6)
P 		303.6
(-3)
P 		406
(0.9)
P 		156
C 639.9
(3)
P 		355.1
(-3)
A 		422
(-1.6)
P 		479.9
(-0.4)
P 		285
avr 535.6
(3) 		337.3
(-3) 		352.8
(-2.5) 		428.5
(-0.2) 		198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 307
1 2 3 4 5 6 range
A 521.1
(3)
P 		352.7
(-0.8)
P 		259.1
(-2.9)
P 		272.4
(-2.6)
P 		269
(-2.6)
P 		252.5
(-3)
P 		269
B 451.7
(3)
P 		352.1
(0.3)
P 		319.5
(-0.6)
P 		254.6
(-2.4)
P 		333
(-0.2)
P 		231.6
(-3)
P 		220
C 364
(1.7)
P 		348.9
(1.2)
P 		404.6
(3)
P 		303.9
(-0.2)
P 		213.8
(-3)
P 		269.2
(-1.3)
P 		191
avr 445.6
(3) 		351.2
(0.1) 		327.7
(-0.6) 		277
(-2.2) 		271.9
(-2.4) 		251.1
(-3) 		195
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 153
1 2 3 4 range
A 184.8
(3)
P 		68.7
(-2.5)
M 		87
(-1.6)
P 		58.5
(-3)
P 		126
B 146.5
(3)
P 		84
(-1.9)
P 		70.4
(-3)
P 		86.5
(-1.7)
P 		76
C 211.8
(3)
P 		102.1
(-2)
A 		147.1
(0)
P 		80.9
(-3)
P 		131
avr 181
(3) 		84.9
(-2.5) 		101.5
(-1.5) 		75.3
(-3) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 96.1
(0.4)
P 		125.5
(3)
P 		56.4
(-3)
P 		69.1
(-1.9)
P 		81.2
(-0.8)
P 		61.5
(-2.6)
P 		69
B 157.2
(3)
P 		117.2
(0.3)
P 		77.6
(-2.3)
P 		67.4
(-3)
P 		135.3
(1.5)
P 		86.4
(-1.7)
P 		90
C 134.9
(3)
P 		106.5
(0.7)
P 		103
(0.4)
P 		61.1
(-3)
P 		69.2
(-2.3)
P 		90.1
(-0.6)
P 		74
avr 129.4
(3) 		116.4
(1.8) 		79
(-1.8) 		65.9
(-3) 		95.2
(-0.2) 		79.3
(-1.7) 		64
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183730_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15960
Sequence Type Exemplar sequence
Representative Public ID F53E2.1 NCBI
Target Description F53E2.1 /REP_DB=WormBase Gene ID /WP=CE20847 /TR=Q9UAU3 /GB=AAD14702.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071023(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071023 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-304) (tag-304) mRNA, complete cds. 11/11 A 
SNAP00000036260 ENSEMBL cdna:SNAP chromosome:CEL140:V:1467596:1470791:1 11/11 None
GENEFINDER00000036265 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1467596:1470791:1 11/11 None
F53E2.1 ENSEMBL cdna:known chromosome:CEL140:V:1467573:1470931:1 gene:F53E2.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1468888-1470791(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-304
Ensembl F53E2.1
Entrez Gene 178639 Entrez gene
SwissProt Q9UAU3 EMBL-EBI
WormBase CE30280 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD14702 Temporarily assigned gene name protein 304 [Caenorhabditis elegans] ref|NP_503424.2| Temporarily Assigned Gene name family member (tag-304) [Caenorhabditis elegans] 0.0
blast CAE62474 Hypothetical protein CBG06570 [Caenorhabditis briggsae] 9.0E-71
blast AAO24554 At1g61150 [Arabidopsis thaliana] 1.0E-13
Sequence
Target Sequence 
>C. ELEGANS:183730_AT
tgaagttcgccgcttgatttgtgttggcgaaatggagagtgctattgaaaaaatgacgac
gctttgcccgactatattggaggatgatgagatcaactttattgttcgcaagcagcatct
cattgaaatggtccgacaaaagctgacgaaagagcccgtcgaatattttagagctcattt
aatgaagaacgggcagcgtccttgtgacgagaaaatggatatcatcgagagaatattcac
tatgctggtattcaatttggaggatgatgtcgagttcaacgtgtatttccagcaatccga
acgagaacagacggccaaggaggtgaacagtgctctgctcgcgatgaatggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAGTTCGCCGCTTGATTTGTGTT 379 573 162 Antisense
GAAAAAATGACGACGCTTTGCCCGA 239 355 208 Antisense
ACGCTTTGCCCGACTATATTGGAGG 673 91 220 Antisense
TATTGTTCGCAAGCAGCATCTCATT 455 665 261 Antisense
GCCCGTCGAATATTTTAGAGCTCAT 352 287 315 Antisense
TGAAGAACGGGCAGCGTCCTTGTGA 390 571 344 Antisense
GTCGAGTTCAACGTGTATTTCCAGC 582 473 430 Antisense
GTATTTCCAGCAATCCGAACGAGAA 636 453 444 Antisense
ACGAGAACAGACGGCCAAGGAGGTG 331 93 462 Antisense
AAGGAGGTGAACAGTGCTCTGCTCG 366 161 478 Antisense
AGTGCTCTGCTCGCGATGAATGGAA 208 61 490 Antisense
Sequence Source Affymetrix Proprietary Database