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[183728_at]

Description F35B12.7 /REP_DB=WormBase Gene ID /WP=CE05806 /TR=Q20011 /GB=CAA98464.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 20      6grp_total > 09      4grp_total > 57      6grp_cgc > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 203
1 2 3 4 range
A 40.2
(-3)
A 		179.9
(3)
A 		146.3
(1.6)
P 		109.9
(-0)
A 		140
B 122.4
(-0)
M 		69.9
(-1.6)
P 		225.1
(3)
P 		22.3
(-3)
A 		203
C 59.2
(-2.2)
A 		223.4
(3)
P 		139.3
(0.4)
P 		32.5
(-3)
A 		191
avr 73.9
(-2) 		157.7
(2.3) 		170.2
(3) 		54.9
(-3) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 155
1 2 3 4 5 6 range
A 115.2
(3)
A 		78.5
(0.5)
A 		104.3
(2.2)
P 		101
(2)
P 		28.5
(-3)
P 		84
(0.8)
P 		87
B 81.7
(3)
A 		80.3
(2.9)
P 		31.2
(-2.2)
A 		42.3
(-1.1)
P 		23.5
(-3)
P 		38.1
(-1.5)
P 		58
C 101.3
(0.3)
P 		21.6
(-2.8)
A 		90.6
(-0.1)
P 		171.7
(3)
P 		83.2
(-0.4)
P 		16.7
(-3)
M 		155
avr 99.4
(2.4) 		60.1
(-1.5) 		75.4
(0) 		105
(3) 		45.1
(-3) 		46.3
(-2.9) 		60
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 14.6
(-3)
A 		39.1
(3)
A 		38.2
(2.8)
P 		16.1
(-2.6)
A 		25
B 39
(1.3)
M 		17.3
(-1.4)
P 		52.2
(3)
P 		4.7
(-3)
A 		48
C 19.6
(-1.6)
A 		64.2
(3)
P 		48.6
(1.4)
P 		5.5
(-3)
A 		59
avr 24.4
(-0.5) 		40.2
(2) 		46.3
(3) 		8.8
(-3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 21.2
(0.9)
A 		27.9
(3)
A 		22.7
(1.4)
P 		25.6
(2.3)
P 		8.6
(-3)
P 		20.4
(0.7)
P 		19
B 28.4
(3)
A 		26.7
(2.5)
P 		7.6
(-3)
A 		11.2
(-2)
P 		9.6
(-2.4)
P 		14.2
(-1.1)
P 		21
C 37.6
(3)
P 		6.6
(-2.8)
A 		23.1
(0.3)
P 		34.5
(2.4)
P 		26.9
(1)
P 		5.6
(-3)
M 		32
avr 29.1
(3) 		20.4
(-0.3) 		17.8
(-1.3) 		23.8
(1) 		15
(-2.4) 		13.4
(-3) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183728_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14249
Sequence Type Exemplar sequence
Representative Public ID F35B12.7 NCBI
Target Description F35B12.7 /REP_DB=WormBase Gene ID /WP=CE05806 /TR=Q20011 /GB=CAA98464.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073545
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073545 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-24) (nlp-24) mRNA, complete cds. None
SNAP00000002155 ENSEMBL cdna:SNAP chromosome:CEL140:V:11618966:11619284:1 None
F35B12.7.2 ENSEMBL cdna:known chromosome:CEL140:V:11618958:11619415:1 gene:F35B12.7 None
F35B12.7.1 ENSEMBL cdna:known chromosome:CEL140:V:11618965:11619419:1 gene:F35B12.7 None
GENEFINDER00000002160 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11618966:11619284:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11618127-11619284(+) 92.89 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-24
Ensembl F35B12.7
Entrez Gene 179594 Entrez gene
SwissProt Q20011 EMBL-EBI
WormBase CE37515 Wormbase
Functional Annotations
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505946.2 1 5-24
F35B12.7.2 1 5-24
F35B12.7.1 1 5-24
SNAP00000002155 1 5-24
GENEFINDER00000002160 1 5-24
Sequence
Target Sequence 
>C. ELEGANS:183728_AT
aacgtgtcggaagcgttccatgcaagggacgtctaccttgtaaaaaacatccgcagcgga
tgttttttgcctgtatgatatttttaataaacattttgaacctaaaatttcgtaaaaagc
gaaacactttcaccagtgaattatcaattcaaatattcttaatgaacaagcttctccttc
tcttcgttcttgttgccgtcatcgctatggcttctgcccaatggggaggtggtccatatg
gaggatatggtccacgtggatacggaggtggatacggaggtggacgttatggaggatatg
gaggacgcggaccatacggcggatacggtggacgtggcccatatggatacggaggacgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGTGTCGGAAGCGTTCCATGCAA 73 143 17 Antisense
GCGTTCCATGCAAGGGACGTCTACC 468 291 29 Antisense
ACCTTGTAAAAAACATCCGCAGCGG 75 85 51 Antisense
ATCCGCAGCGGATGTTTTTTGCCTG 74 39 65 Antisense
GGATGTTTTTTGCCTGTATGATATT 183 515 74 Antisense
AAAGCGAAACACTTTCACCAGTGAA 419 125 132 Antisense
TCTTAATGAACAAGCTTCTCCTTCT 56 615 173 Antisense
TCTGCCCAATGGGGAGGTGGTCCAT 269 583 229 Antisense
GCGGACCATACGGCGGATACGGTGG 156 293 323 Antisense
ATACGGTGGACGTGGCCCATATGGA 629 21 339 Antisense
GGCCCATATGGATACGGAGGACGTG 692 541 352 Antisense
Sequence Source Affymetrix Proprietary Database