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[183710_at]

Description R01H2.5 /REP_DB=WormBase Gene ID /WP=CE00804 /TR=Q21632 /GB=AAA50647.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 06      6grp_high1_10_10 > 50      4grp_highest_8_8 > 08      4grp_high_8_8 > 52      6grp_highest_nonorm > 33      6grp_total > 40      4grp_total > 59      6grp_highest_sub > 04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1759
1 2 3 4 range
A 1110.3
(-0.2)
P 		2049.5
(3)
P 		1577.7
(1.4)
P 		290.3
(-3)
P 		1759
B 1368.8
(0.8)
P 		1842.9
(3)
P 		1605.2
(1.9)
P 		572.2
(-3)
P 		1271
C 1357.6
(0.8)
P 		1682.2
(2.3)
P 		1839.3
(3)
P 		524.5
(-3)
P 		1315
avr 1278.9
(0.5) 		1858.2
(3) 		1674.1
(2.2) 		462.3
(-3) 		1396

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1802
1 2 3 4 5 6 range
A 2191.2
(1.5)
P 		1862.8
(0.4)
P 		2628.6
(3)
P 		2498
(2.5)
P 		1416.7
(-1.2)
P 		889.5
(-3)
P 		1739
B 1479.6
(-1.2)
P 		2203.7
(1.4)
P 		2645.8
(3)
P 		2261.8
(1.6)
P 		1555.5
(-0.9)
P 		974
(-3)
P 		1672
C 1618.7
(-1)
P 		2222.7
(1.3)
P 		2192.9
(1.1)
P 		2691.4
(3)
P 		1356.8
(-2)
P 		1075.2
(-3)
P 		1616
avr 1763.2
(0.1) 		2096.4
(1.4) 		2489.1
(3) 		2483.7
(3) 		1443
(-1.2) 		979.6
(-3) 		1510
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 599
1 2 3 4 range
A 404.3
(2.4)
P 		444.9
(3)
P 		412.4
(2.5)
P 		42.5
(-3)
P 		402
B 436.5
(2.7)
P 		455.1
(3)
P 		372
(1.5)
P 		121.9
(-3)
P 		333
C 449.4
(0.9)
P 		483.6
(1.3)
P 		641.1
(3)
P 		88.4
(-3)
P 		553
avr 430.1
(2.3) 		461.2
(2.8) 		475.2
(3) 		84.3
(-3) 		391

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 517
1 2 3 4 5 6 range
A 403.9
(-0.5)
P 		662.5
(3)
P 		572.4
(1.8)
P 		633.5
(2.6)
P 		427.5
(-0.2)
P 		216.6
(-3)
P 		446
B 514.8
(-0.5)
P 		733.5
(3)
P 		642.5
(1.5)
P 		599.1
(0.8)
P 		632
(1.4)
P 		363.2
(-3)
P 		370
C 600
(1.5)
P 		678.2
(3)
P 		558.1
(0.7)
P 		541.2
(0.4)
P 		439.1
(-1.5)
P 		359.9
(-3)
P 		318
avr 506.2
(0.1) 		691.4
(3) 		591
(1.4) 		591.3
(1.4) 		499.5
(-0) 		313.2
(-3) 		378
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183710_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7128
Sequence Type Exemplar sequence
Representative Public ID R01H2.5 NCBI
Target Description R01H2.5 /REP_DB=WormBase Gene ID /WP=CE00804 /TR=Q21632 /GB=AAA50647.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066139(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066139 NCBI Caenorhabditis elegans R01H2.5 (R01H2.5) mRNA, complete cds. 11/11 None
GENEFINDER00000040678 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7065471:7067366:-1 11/11 None
SNAP00000040675 ENSEMBL cdna:SNAP chromosome:CEL140:III:7065489:7067366:-1 11/11 None
R01H2.5 ENSEMBL cdna:known chromosome:CEL140:III:7065489:7067366:-1 gene:R01H2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7065486-7067364(-) 99.26 100.0
Public Domain and Genome References
Gene Title
Ensembl R01H2.5
Entrez Gene 187512 Entrez gene
SwissProt Q21632 EMBL-EBI
WormBase CE00804 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1583827_AT similar to GDP-L-fucose synthetase (FX protein) (Red cell NADP(H)-binding protein) (GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase) cfa
CANINE_2:CFA.12200.1.A1_AT similar to GDP-L-fucose synthetase (FX protein) (Red cell NADP(H)-binding protein) (GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase) cfa
CANINE_2:CFAAFFX.2894.1.S1_AT similar to GDP-L-fucose synthetase (FX protein) (Red cell NADP(H)-binding protein) (GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase) cfa
DROSOPHILA_2:1626180_AT GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase/4-reductase dm
DROSGENOME1:153173_AT GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase/4-reductase dm
CHICKEN:GGA.12666.1.S1_AT tissue specific transplantation antigen P35B gga
HG-U133A:201644_AT tissue specific transplantation antigen P35B hs
HG-U133_PLUS_2:201644_AT tissue specific transplantation antigen P35B hs
HG-U133A_2:201644_AT tissue specific transplantation antigen P35B hs
HUGENEFL:U58766_AT tissue specific transplantation antigen P35B hs
U133_X3P:G6598326_3P_S_AT tissue specific transplantation antigen P35B hs
U133_X3P:G6598326_3P_AT tissue specific transplantation antigen P35B hs
U133_X3P:201644_3P_AT tissue specific transplantation antigen P35B hs
HG-U133A_2:36936_AT tissue specific transplantation antigen P35B hs
HG-FOCUS:36936_AT tissue specific transplantation antigen P35B hs
HG-U133_PLUS_2:36936_AT tissue specific transplantation antigen P35B hs
HG-U133A:36936_AT tissue specific transplantation antigen P35B hs
HG-U95AV2:36936_AT tissue specific transplantation antigen P35B hs
MU11KSUBA:M30127_S_AT tissue specific transplantation antigen P35B mm
MG-U74AV2:94298_AT tissue specific transplantation antigen P35B mm
MG-U74AV2:102113_F_AT tissue specific transplantation antigen P35B mm
MOE430A:1448495_AT tissue specific transplantation antigen P35B mm
MOUSE430A_2:1448495_AT tissue specific transplantation antigen P35B mm
MOUSE430_2:1448495_AT tissue specific transplantation antigen P35B mm
RG-U34C:RC_AI177025_AT tissue specific transplantation antigen P35B (predicted) rn
RAE230A:1398953_AT tissue specific transplantation antigen P35B (predicted) rn
RAT230_2:1398953_AT tissue specific transplantation antigen P35B (predicted) rn
Protein Similarities
Method ID Description E-Value
blast NP_498540 R01H2.5 [Caenorhabditis elegans] gb|AAA50647.1| Hypothetical protein R01H2.5 [Caenorhabditis elegans] 1.0E-176
blast CAE70071 Hypothetical protein CBG16511 [Caenorhabditis briggsae] 1.0E-165
blast AAH86741 Zgc:101805 [Danio rerio] ref|NP_001008620.1| zgc:101805 [Danio rerio] emb|CAI21010.1| tissue specific transplantation antigen P35B [Danio rerio] ref|XP_685381.1| PREDICTED: similar to tissue specific transplantation antigen P35B isoform 1 [Danio rerio] 1.0E-118
Sequence
Target Sequence 
>C. ELEGANS:183710_AT
tgtctacatgtattttccctgataaaacctcgtatcctatcgatgagacaatggttcatc
taggtccacctcatgattccaactttggatattcgtatgccaaacgaatgattgatgttt
tgaataagggatatgcacaggaacacggaagaaaatacacatctgttgttccttgtaatg
tctttggacctcatgacaactataatctacaatcaggacatgttctaccggcattgattc
ataaagcatatgttgctcaaagagatggaactccactgcaagtatatggatctggaacac
cattgagacagttcatttactctatcgacttggcgagacttttcattcgagttgtcaggg
agtacgaggatgttgaaccaattattctgtctgtcaatgaatctgatgaagtttcaattc
gtgatgctgtttctgctgttgtcaaagcaattgatttcactggagacgtcgaatacgata
cgtccaaggctgatggacaattcaagaagactgcttctaatgagaaacttctgaaattat
tccccgattttcaattcacgccattcgagcaggctat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTCTACATGTATTTTCCCTGATAA 681 557 332 Antisense
AAACCTCGTATCCTATCGATGAGAC 465 125 356 Antisense
ACAATGGTTCATCTAGGTCCACCTC 252 113 379 Antisense
GTCCACCTCATGATTCCAACTTTGG 530 475 395 Antisense
ATCTGTTGTTCCTTGTAATGTCTTT 606 31 492 Antisense
GGACATGTTCTACCGGCATTGATTC 9 527 547 Antisense
GTTCATTTACTCTATCGACTTGGCG 650 443 642 Antisense
GGCGAGACTTTTCATTCGAGTTGTC 445 539 663 Antisense
AATTCGTGATGCTGTTTCTGCTGTT 474 177 747 Antisense
ATTATTCCCCGATTTTCAATTCACG 585 13 867 Antisense
AATTCACGCCATTCGAGCAGGCTAT 73 177 884 Antisense
Sequence Source Affymetrix Proprietary Database