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[183702_at]

Description K05B2.4 /REP_DB=WormBase Gene ID /WP=CE02802 /TR=Q9GYN3 /GB=AAF99980.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 47      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 70      6grp_total > 48      4grp_total > 0      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 716
1 2 3 4 range
A 404.1
(-1.2)
P 		236.3
(-3)
P 		656.3
(1.6)
P 		787.7
(3)
P 		551
B 313.4
(-2.1)
P 		204.4
(-3)
P 		597.8
(0.3)
P 		920.8
(3)
P 		716
C 388.2
(-1.6)
P 		262.2
(-3)
P 		733.8
(2.3)
P 		791.9
(3)
P 		530
avr 368.6
(-1.7) 		234.3
(-3) 		662.6
(1.3) 		833.5
(3) 		599

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 550
1 2 3 4 5 6 range
A 530.5
(3)
P 		169.5
(-1.5)
P 		153.1
(-1.7)
P 		105.9
(-2.3)
P 		52.3
(-3)
P 		506
(2.7)
P 		478
B 602.3
(3)
P 		170.7
(-1.8)
P 		108
(-2.5)
P 		102.1
(-2.6)
P 		64.2
(-3)
P 		535.9
(2.3)
P 		538
C 552.4
(3)
P 		126.4
(-2.2)
P 		129.5
(-2.2)
P 		97.8
(-2.5)
P 		60.2
(-3)
P 		484.4
(2.2)
P 		492
avr 561.7
(3) 		155.5
(-1.8) 		130.2
(-2.1) 		101.9
(-2.5) 		58.9
(-3) 		508.8
(2.4) 		503
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 205
1 2 3 4 range
A 147.1
(1.8)
P 		51.3
(-3)
P 		171.6
(3)
P 		115.3
(0.2)
P 		120
B 99.9
(-1)
P 		50.5
(-3)
P 		138.5
(0.6)
P 		196.2
(3)
P 		146
C 128.5
(-1.2)
P 		75.4
(-3)
P 		255.8
(3)
P 		133.5
(-1.1)
P 		180
avr 125.2
(0.1) 		59.1
(-3) 		188.6
(3) 		148.3
(1.1) 		130

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 194
1 2 3 4 5 6 range
A 97.8
(1.6)
P 		60.3
(-0.5)
P 		33.3
(-2)
P 		26.8
(-2.4)
P 		15.8
(-3)
P 		123.2
(3)
P 		107
B 209.6
(3)
P 		56.8
(-2)
P 		26.2
(-3)
P 		27
(-3)
P 		26.1
(-3)
P 		199.8
(2.7)
P 		184
C 204.8
(3)
P 		38.6
(-2.4)
P 		33
(-2.6)
P 		19.7
(-3)
P 		19.5
(-3)
P 		162.2
(1.6)
P 		185
avr 170.7
(3) 		51.9
(-1.7) 		30.8
(-2.6) 		24.5
(-2.8) 		20.5
(-3) 		161.7
(2.6) 		150
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183702_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17048
Sequence Type Exemplar sequence
Representative Public ID K05B2.4 NCBI
Target Description K05B2.4 /REP_DB=WormBase Gene ID /WP=CE02802 /TR=Q9GYN3 /GB=AAF99980.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076436(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076436 NCBI Caenorhabditis elegans K05B2.4 (K05B2.4) mRNA, complete cds. 11/11 None
SNAP00000040752 ENSEMBL cdna:SNAP chromosome:CEL140:X:4919022:4920946:-1 11/11 None
GENEFINDER00000040754 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4919022:4929724:-1 11/11 None
K05B2.4 ENSEMBL cdna:known chromosome:CEL140:X:4919022:4920946:-1 gene:K05B2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4919022-4920947(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K05B2.4
Entrez Gene 187017 Entrez gene
SwissProt Q9GYN3 EMBL-EBI
WormBase CE35738 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGA.11254.1.S1_AT similar to peroxisomal acyl-CoA thioesterase Ic gga
MOUSE430_2:1422925_S_AT acyl-CoA thioesterase 3 mm
MOE430A:1422925_S_AT acyl-CoA thioesterase 3 mm
MOUSE430A_2:1422925_S_AT acyl-CoA thioesterase 3 mm
MG-U74BV2:112689_AT acyl-CoA thioesterase 3 mm
MU19KSUBC:TC38745_AT Acyl-CoA thioesterase 3 (Acot3), mRNA mm
MU19KSUBC:TC41428_AT Acyl-CoA thioesterase 3 (Acot3), mRNA mm
RAE230A:1377037_AT similar to peroxisomal long chain acyl-CoA thioesterase Ia rn
RAT230_2:1377037_AT similar to peroxisomal long chain acyl-CoA thioesterase Ia rn
RAE230B:1378169_AT similar to peroxisomal long chain acyl-CoA thioesterase Ia rn
RAT230_2:1378169_AT similar to peroxisomal long chain acyl-CoA thioesterase Ia rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6629 lipid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16290 palmitoyl-CoA hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF99980 Hypothetical protein K05B2.4 [Caenorhabditis elegans] ref|NP_508837.2| K05B2.4 [Caenorhabditis elegans] 0.0
blast T16564 hypothetical protein K05B2.5 - Caenorhabditis elegans 0.0
blast AAF99981 Patterned expression site protein 22 [Caenorhabditis elegans] ref|NP_508838.3| Patterned Expression Site family member (pes-22) [Caenorhabditis elegans] 0.0
blast CAE68667 Hypothetical protein CBG14568 [Caenorhabditis briggsae] 0.0
blast CAE68666 Hypothetical protein CBG14567 [Caenorhabditis briggsae] 1.0E-113
blast CAB07846 Hypothetical protein C31H5.6 [Caenorhabditis elegans] ref|NP_492431.1| C31H5.6 [Caenorhabditis elegans] 7.0E-87
Protein Domains
Database ID Description E-Value
Pfam IPR006862 EMBL-EBI Acyl-CoA thioester hydrolase/bile acid-CoA amino acid N-acetyltransferase 2.3E-87
Pfam IPR006862 EMBL-EBI Acyl-CoA thioester hydrolase/bile acid-CoA amino acid N-acetyltransferase 2.3E-87
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000040754 12 44-66,79-101,111-133,140-162,167-189,202-219,480-502,515-534,544-566,573-595,610-629,638-660
Sequence
Target Sequence 
>C. ELEGANS:183702_AT
tttgtcacttcattaccctatacgaatgacaggattggctttcaaggtatatcgtttgga
ggaactcttgttatgtttctcaccacaaggttcaaacagcatttcagatttatcaatcaa
ggaattgaggatatctggtatttgaatgatttaatggtgtacactgatatggtcagaaac
ctaaaacttgaagatggggcaggatttaaattcgaggattcaagtccagaaactgcattt
agattttctctcgccgtagatgatttgagcacaccaactgtatttgtaggaaaggtgtta
tcagaaaagttgagaaacttaaacaggaaagtcgaagttcattatgtttctggtggtcat
ttgctagatcctccatgttttccgcatcacgaagctgtgttttcagcttttgcaggaacc
ttccaagcatatggtggtgaaacaagccttcacggaaaaagtcagtttgaagtctgggaa
aaaactgtccaatttttctccgaacacctcgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGTCACTTCATTACCCTATACGA 577 667 619 Antisense
GAACTCTTGTTATGTTTCTCACCAC 519 349 680 Antisense
TGCATTTAGATTTTCTCTCGCCGTA 159 579 852 Antisense
TCGCCGTAGATGATTTGAGCACACC 417 599 869 Antisense
GAGCACACCAACTGTATTTGTAGGA 619 389 885 Antisense
AAGTTCATTATGTTTCTGGTGGTCA 72 157 953 Antisense
TGGTGGTCATTTGCTAGATCCTCCA 89 553 969 Antisense
CATGTTTTCCGCATCACGAAGCTGT 494 205 992 Antisense
GTTTTCAGCTTTTGCAGGAACCTTC 75 447 1017 Antisense
GGAACCTTCCAAGCATATGGTGGTG 459 529 1033 Antisense
TCCAATTTTTCTCCGAACACCTCGG 67 587 1106 Antisense
Sequence Source Affymetrix Proprietary Database