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[183700_s_at]

Description F35E8.13 /REP_DB=WormBase Gene ID /WP=CE15960 /TR=O46357 /GB=CAB04297.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 08      4grp_total > 47      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2714
1 2 3 4 range
A 2642.4
(3)
P 		93.1
(-2.9)
A 		54.8
(-3)
A 		48.6
(-3)
A 		2594
B 2735.6
(3)
P 		84
(-2.9)
A 		61
(-2.9)
A 		26.5
(-3)
A 		2709
C 2740.9
(3)
P 		113.3
(-2.8)
A 		31.5
(-3)
A 		78.4
(-2.9)
A 		2709
avr 2706.3
(3) 		96.8
(-2.9) 		49.1
(-3) 		51.2
(-3) 		2657

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 304
1 2 3 4 5 6 range
A 287.7
(3)
A 		43.3
(-2.2)
A 		78.5
(-1.4)
P 		88
(-1.2)
A 		59.3
(-1.8)
A 		4.2
(-3)
A 		284
B 252.9
(3)
P 		62.3
(-2.2)
A 		86.1
(-1.5)
A 		54.2
(-2.4)
A 		42.9
(-2.7)
A 		31.1
(-3)
A 		222
C 307.7
(3)
P 		52.9
(-2.5)
A 		70.6
(-2.1)
A 		65.5
(-2.2)
A 		29.5
(-3)
A 		45.2
(-2.7)
A 		278
avr 282.8
(3) 		52.8
(-2.4) 		78.4
(-1.8) 		69.2
(-2) 		43.9
(-2.6) 		26.8
(-3) 		256
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 956
1 2 3 4 range
A 962.1
(3)
P 		20.2
(-2.9)
A 		14.3
(-3)
A 		7.1
(-3)
A 		955
B 872.3
(3)
P 		20.8
(-2.9)
A 		14.1
(-2.9)
A 		5.7
(-3)
A 		867
C 907.3
(3)
P 		32.6
(-2.9)
A 		11
(-3)
A 		13.2
(-3)
A 		896
avr 913.9
(3) 		24.5
(-2.9) 		13.1
(-3) 		8.7
(-3) 		905

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 53
(3)
A 		15.4
(-1.3)
A 		17.1
(-1.1)
P 		22.3
(-0.5)
A 		17.9
(-1.1)
A 		1
(-3)
A 		52
B 88
(3)
P 		20.7
(-2.3)
A 		20.9
(-2.3)
A 		14.3
(-2.8)
A 		17.4
(-2.5)
A 		11.6
(-3)
A 		76
C 114.1
(3)
P 		16.2
(-2.6)
A 		18
(-2.5)
A 		13.2
(-2.8)
A 		9.6
(-3)
A 		15.1
(-2.7)
A 		105
avr 85
(3) 		17.4
(-2.4) 		18.7
(-2.2) 		16.6
(-2.4) 		15
(-2.5) 		9.2
(-3) 		76
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 183700_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14308
Sequence Type Exemplar sequence
Representative Public ID F35E8.13 NCBI
Target Description F35E8.13 /REP_DB=WormBase Gene ID /WP=CE15960 /TR=O46357 /GB=CAB04297.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074584(11), NM_074587(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074584 NCBI Caenorhabditis elegans F35E8.10 (F35E8.10) mRNA, complete cds. 11/11 None
NM_074587 NCBI Caenorhabditis elegans F35E8.13 (F35E8.13) mRNA, complete cds. 11/11 None
SNAP00000019516 ENSEMBL cdna:SNAP chromosome:CEL140:V:15922018:15922928:-1 11/11 None
SNAP00000019519 ENSEMBL cdna:SNAP chromosome:CEL140:V:15927828:15928738:1 11/11 None
GENEFINDER00000019523 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15922018:15922928:-1 11/11 None
GENEFINDER00000019526 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15927828:15928738:1 11/11 None
F35E8.10 ENSEMBL cdna:known chromosome:CEL140:V:15922018:15922928:-1 gene:F35E8.10 11/11 None
F35E8.13 ENSEMBL cdna:known chromosome:CEL140:V:15927828:15928738:1 gene:F35E8.13 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15927827-15928738(+) 100.0 100.0
chrV:15922017-15922928(-) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl F35E8.10
F35E8.13
Entrez Gene 185301 Entrez gene
185302 Entrez gene
SwissProt O46357 EMBL-EBI
WormBase CE15960 Wormbase
CE15960 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04297 Hypothetical protein F35E8.13 [Caenorhabditis elegans] emb|CAB04291.1| Hypothetical protein F35E8.10 [Caenorhabditis elegans] ref|NP_506988.1| F35E8.13 [Caenorhabditis elegans] ref|NP_506985.1| F35E8.10 [Caenorhabditis elegans] 1.0E-134
blast CAB03300 Hypothetical protein T06C12.14 [Caenorhabditis elegans] ref|NP_506976.1| T06C12.14 [Caenorhabditis elegans] 1.0E-112
blast CAB04292 Hypothetical protein F35E8.9 [Caenorhabditis elegans] ref|NP_506984.1| F35E8.9 [Caenorhabditis elegans] 1.0E-112
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 6.5E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.5E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.4E-11
Sequence
Target Sequence 
>C. ELEGANS:183700_S_AT
gcagctgacggatatccatcaccaggaagagatcagcagagaccattggcttgttatacg
actgctaccgcgactccagctgctgtagttcaagacatgaagaatgcagctcagagcaca
tgcccaagaacttgcggattatgttgtcagacttctggttacaactgcccaaatgttgca
tatccacgtctcaactgtggcaccatcaccgcctcccaatgtctgtcaccggtttggaga
ccaattatcgctgcggactgtccatctgcctgcggattctgcaatgagggaggatgcgtt
gatgctattcccgactgtgcaaatgacataagaatttgcaccgccgctggactcgaaacc
tttgtggccacctactgccagagaacctgtggaaaatgtgcatcttcaacaactacccga
tcatctgcttctactggaacctgttcttctttcatcgctgataccagtccatcatgtgct
gcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAGCTGACGGATATCCATCACCAG 337 313 193 Antisense
GTCAGACTTCTGGTTACAACTGCCC 629 465 338 Antisense
TCCCAATGTCTGTCACCGGTTTGGA 586 597 406 Antisense
AATTATCGCTGCGGACTGTCCATCT 392 175 435 Antisense
AGGATGCGTTGATGCTATTCCCGAC 193 57 483 Antisense
GACATAAGAATTTGCACCGCCGCTG 502 369 517 Antisense
TGGACTCGAAACCTTTGTGGCCACC 230 549 540 Antisense
AATGTGCATCTTCAACAACTACCCG 670 161 587 Antisense
ATCATCTGCTTCTACTGGAACCTGT 462 29 612 Antisense
TTCTTCTTTCATCGCTGATACCAGT 179 691 636 Antisense
TGATACCAGTCCATCATGTGCTGCC 245 565 651 Antisense
Sequence Source Affymetrix Proprietary Database