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[183694_at]

Description F36A4.8 /REP_DB=WormBase Gene ID /WP=CE07184 /TR=Q20089 /GB=AAA96157.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 17      6grp_total > 07      4grp_total > 52      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 222
1 2 3 4 range
A 213.8
(3)
P 		156.5
(1.1)
P 		116.7
(-0.2)
A 		32.2
(-3)
A 		182
B 174.4
(1.8)
P 		200.6
(3)
P 		149.3
(0.7)
A 		68.5
(-3)
A 		132
C 179.1
(0.3)
P 		219.8
(1.8)
P 		253.9
(3)
P 		89.3
(-3)
A 		165
avr 189.1
(2.9) 		192.3
(3) 		173.3
(2.1) 		63.3
(-3) 		129

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 443
1 2 3 4 5 6 range
A 230.8
(3)
P 		193
(0.9)
P 		190.2
(0.7)
P 		137.6
(-2.3)
P 		206.8
(1.6)
A 		124.9
(-3)
P 		106
B 568.2
(3)
P 		284.5
(-1.1)
P 		255.3
(-1.5)
P 		150.1
(-3)
M 		255.2
(-1.5)
P 		182.6
(-2.5)
P 		418
C 361.5
(3)
P 		275.5
(0.5)
P 		190.1
(-2)
M 		236.4
(-0.7)
P 		156.7
(-3)
P 		205.7
(-1.6)
P 		205
avr 386.8
(3) 		251
(-0.8) 		211.9
(-1.9) 		174.7
(-2.9) 		206.2
(-2) 		171.1
(-3) 		216
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 77.9
(3)
P 		34
(-0.6)
P 		30.5
(-0.9)
A 		4.7
(-3)
A 		73
B 55.6
(3)
P 		49.5
(2.1)
P 		34.6
(-0.1)
A 		14.6
(-3)
A 		41
C 59.3
(0.6)
P 		63.2
(0.9)
P 		88.5
(3)
P 		15.1
(-3)
A 		73
avr 64.3
(3) 		48.9
(1.3) 		51.2
(1.5) 		11.5
(-3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 167
1 2 3 4 5 6 range
A 42.5
(-1.1)
P 		68.6
(3)
P 		41.4
(-1.3)
P 		34.9
(-2.3)
P 		62.4
(2)
A 		30.4
(-3)
P 		38
B 197.7
(3)
P 		94.7
(-0.9)
P 		62
(-2.2)
P 		39.8
(-3)
M 		103.7
(-0.6)
P 		68.1
(-1.9)
P 		158
C 134
(3)
P 		84.1
(-0.5)
P 		48.4
(-2.9)
M 		47.5
(-3)
P 		50.7
(-2.8)
P 		68.9
(-1.5)
P 		87
avr 124.7
(3) 		82.5
(-0) 		50.6
(-2.3) 		40.7
(-3) 		72.3
(-0.7) 		55.8
(-1.9) 		84
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183694_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10630
Sequence Type Exemplar sequence
Representative Public ID F36A4.8 NCBI
Target Description F36A4.8 /REP_DB=WormBase Gene ID /WP=CE07184 /TR=Q20089 /GB=AAA96157.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068121(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068121 NCBI Caenorhabditis elegans F36A4.8 (F36A4.8) mRNA, complete cds. 11/11 None
SNAP00000033258 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4246914:4247396:1 11/11 None
GENEFINDER00000033278 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4246914:4248121:1 11/11 None
F36A4.8.1 ENSEMBL cdna:known chromosome:CEL140:IV:4245165:4247396:1 gene:F36A4.8 11/11 None
F36A4.8.2 ENSEMBL cdna:known chromosome:CEL140:IV:4246772:4247396:1 gene:F36A4.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4246919-4247402(+) 98.62 100.0
Public Domain and Genome References
Gene Title
Ensembl F36A4.8
Entrez Gene 177189 Entrez gene
SwissProt Q20089 EMBL-EBI
WormBase CE07184 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA96157 Hypothetical protein F36A4.8 [Caenorhabditis elegans] ref|NP_500522.1| F36A4.8 [Caenorhabditis elegans] 4.0E-80
blast AAA96157 Hypothetical protein F36A4.8 [Caenorhabditis elegans] ref|NP_500522.1| F36A4.8 [Caenorhabditis elegans] 1.0E-77
blast CAE61464 Hypothetical protein CBG05356 [Caenorhabditis briggsae] 2.0E-69
blast CAE61464 Hypothetical protein CBG05356 [Caenorhabditis briggsae] 6.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR001534 EMBL-EBI Transthyretin-like 1.9E-70
Pfam IPR001534 EMBL-EBI Transthyretin-like 1.1E-68
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500522.1 1 4-26
F36A4.8.1 1 4-26
F36A4.8.2 1 4-26
SNAP00000033258 1 4-26
GENEFINDER00000033278 1 4-26
Sequence
Target Sequence 
>C. ELEGANS:183694_AT
gcaactattcctgatcttagccatccttgcctactctgtgcaaggccttctcggaatcgg
acggctccagtcggttgccgtcagcggccggctcatctgtgatggccgaccggcggctgg
agtgaaggttaagatgtatgagaaagagtttttcctcgatcgaaaaatggccgaagtgta
cacggatgttaacggcgttttccagattactggtagaaaacgtgaaatttcgactatcga
cccgaaggtgaacgtataccataagtgtaattacgctggaatctgctataaaaagtttgg
tatcacaattcccgacgactatatcacttggggatactcgccgaacagaaattacgatat
tggcactctaaacttggcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAACTATTCCTGATCTTAGCCATC 315 319 30 Antisense
CCATCCTTGCCTACTCTGTGCAAGG 629 271 50 Antisense
CAAGGCCTTCTCGGAATCGGACGGC 55 183 70 Antisense
GCCGGCTCATCTGTGATGGCCGACC 551 281 116 Antisense
CCGACCGGCGGCTGGAGTGAAGGTT 290 257 135 Antisense
GTTAACGGCGTTTTCCAGATTACTG 129 437 217 Antisense
TCGACTATCGACCCGAAGGTGAACG 616 249 259 Antisense
AAGTTTGGTATCACAATTCCCGACG 504 155 322 Antisense
AATTCCCGACGACTATATCACTTGG 420 177 336 Antisense
TCACTTGGGGATACTCGCCGAACAG 175 625 353 Antisense
GATATTGGCACTCTAAACTTGGCAA 680 425 385 Antisense
Sequence Source Affymetrix Proprietary Database