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[183686_at]

Description F53H1.3 /REP_DB=WormBase Gene ID /WP=CE17861 /TR=O45074 /GB=AAC02577.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_total > 29      4grp_total > 53      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 201
1 2 3 4 range
A 239.3
(3)
P 		211.9
(1.6)
P 		193.3
(0.6)
P 		124
(-3)
P 		115
B 231
(2.7)
P 		233.5
(3)
P 		190.7
(-1.4)
P 		175
(-3)
P 		59
C 319.3
(3)
P 		237.6
(0.6)
P 		195
(-0.7)
P 		118.7
(-3)
P 		201
avr 263.2
(3) 		227.7
(1.3) 		193
(-0.4) 		139.2
(-3) 		124

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 153
1 2 3 4 5 6 range
A 192.5
(0.8)
M 		239
(3)
P 		110.7
(-3)
A 		135.9
(-1.8)
M 		208.1
(1.6)
P 		156.8
(-0.8)
P 		128
B 183.9
(0)
P 		145.1
(-2.9)
P 		143.6
(-3)
P 		181
(-0.2)
A 		223.5
(3)
P 		186.4
(0.2)
P 		80
C 237.1
(1.8)
P 		165.5
(-1.5)
P 		169.9
(-1.3)
A 		133.4
(-3)
A 		263.3
(3)
P 		134.4
(-3)
A 		130
avr 204.5
(1.2) 		183.2
(-0.2) 		141.4
(-3) 		150.1
(-2.4) 		231.6
(3) 		159.2
(-1.8) 		90
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 87.1
(3)
P 		46
(-0.6)
P 		50.5
(-0.2)
P 		18.2
(-3)
P 		69
B 73.7
(3)
P 		57.7
(0.4)
P 		44.2
(-1.9)
P 		37.3
(-3)
P 		36
C 105.7
(3)
P 		68.3
(0.4)
P 		68
(0.4)
P 		20
(-3)
P 		86
avr 88.8
(3) 		57.3
(0) 		54.2
(-0.3) 		25.2
(-3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 35.5
(-1.9)
M 		85
(3)
P 		24.1
(-3)
A 		34.5
(-2)
M 		62.8
(0.8)
P 		38.2
(-1.6)
P 		61
B 64
(0.1)
P 		48.3
(-1.6)
P 		34.9
(-3)
P 		48
(-1.6)
A 		90.8
(3)
P 		69.5
(0.7)
P 		56
C 87.9
(3)
P 		50.5
(-0.7)
P 		43.2
(-1.4)
A 		26.8
(-3)
A 		85.2
(2.7)
P 		45
(-1.2)
A 		61
avr 62.5
(0.7) 		61.3
(0.6) 		34.1
(-3) 		36.4
(-2.7) 		79.6
(3) 		50.9
(-0.8) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183686_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11061
Sequence Type Exemplar sequence
Representative Public ID F53H1.3 NCBI
Target Description F53H1.3 /REP_DB=WormBase Gene ID /WP=CE17861 /TR=O45074 /GB=AAC02577.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067664(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067664 NCBI Caenorhabditis elegans F53H1.3 (F53H1.3) mRNA, complete cds. 10/11 A 
SNAP00000013766 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1312127:1336434:1 10/11 None
GENEFINDER00000013784 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1331469:1335356:1 9/11 None
F53H1.3 ENSEMBL cdna:known chromosome:CEL140:IV:1329160:1339370:1 gene:F53H1.3 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1329168-1335495(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53H1.3
Entrez Gene 176945 Entrez gene
SwissProt O45074 EMBL-EBI
WormBase CE31538 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:260377_AT expressed protein at
CANINE_2:CFAAFFX.12467.1.S1_S_AT similar to F53H1.3 cfa
CHICKEN:GGA.13171.1.S1_S_AT similar to hypothetical protein FLJ34969 gga
CHICKEN:GGA.2300.1.S1_AT similar to hypothetical protein FLJ34969 gga
CHICKEN:GGA.6997.1.S1_S_AT similar to hypothetical protein FLJ34969 gga
CHICKEN:GGA.6997.2.S1_S_AT similar to hypothetical protein FLJ34969 gga
CHICKEN:GGAAFFX.3549.1.S1_AT similar to hypothetical protein FLJ34969 gga
CHICKEN:GGA.6997.2.A1_AT Similar to hypothetical protein FLJ34969 gga
HU35KSUBA:HUM_ALU_AT hypothetical protein FLJ34969 hs
HU35KSUBB:HUM_ALU_AT hypothetical protein FLJ34969 hs
HU35KSUBC:HUM_ALU_AT hypothetical protein FLJ34969 hs
HU35KSUBD:HUM_ALU_AT hypothetical protein FLJ34969 hs
HUGENEFL:HUM_ALU_AT hypothetical protein FLJ34969 hs
HC-G110:HUM_ALU_AT hypothetical protein FLJ34969 hs
HU35KSUBC:RC_T93865_AT hypothetical protein FLJ34969 hs
HU35KSUBC:RC_N52349_AT hypothetical protein FLJ34969 hs
HU35KSUBD:RC_AA291672_S_AT hypothetical protein FLJ34969 hs
HU35KSUBD:RC_AA287553_I_AT hypothetical protein FLJ34969 hs
HU35KSUBD:RC_AA287553_S_AT hypothetical protein FLJ34969 hs
U133_X3P:HS.99670.0.A1_3P_AT hypothetical protein FLJ34969 hs
HG-U133_PLUS_2:226965_AT hypothetical protein FLJ34969 hs
HG-U133B:226965_AT hypothetical protein FLJ34969 hs
HG-U95C:63303_AT hypothetical protein FLJ34969 hs
HG-U95D:70783_AT hypothetical protein FLJ34969 hs
MOUSE430_2:1437253_AT RIKEN cDNA A630054L15 gene mm
MOE430A:1437253_AT RIKEN cDNA A630054L15 gene mm
MOUSE430A_2:1437253_AT RIKEN cDNA A630054L15 gene mm
MOUSE430A_2:1451568_AT RIKEN cDNA A630054L15 gene mm
MOE430A:1451568_AT RIKEN cDNA A630054L15 gene mm
MOUSE430_2:1451568_AT RIKEN cDNA A630054L15 gene mm
MG-U74BV2:112108_AT RIKEN cDNA A630054L15 gene mm
RG-U34B:RC_AA851364_AT similar to RIKEN cDNA A630054L15; hypothetical protein MGC38041 (predicted) rn
RG-U34C:RC_AI235901_AT similar to RIKEN cDNA A630054L15; hypothetical protein MGC38041 (predicted) rn
RAE230A:1399150_AT similar to RIKEN cDNA A630054L15; hypothetical protein MGC38041 (predicted) rn
RAT230_2:1399150_AT similar to RIKEN cDNA A630054L15; hypothetical protein MGC38041 (predicted) rn
RAE230B:1383209_AT similar to RIKEN cDNA A630054L15; hypothetical protein MGC38041 (predicted) rn
RAT230_2:1383209_AT similar to RIKEN cDNA A630054L15; hypothetical protein MGC38041 (predicted) rn
RG-U34B:RC_AI045596_AT Similar to RIKEN cDNA A630054L15; hypothetical protein MGC38041 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAC02577 Hypothetical protein F53H1.3 [Caenorhabditis elegans] ref|NP_500065.2| F53H1.3 [Caenorhabditis elegans] 0.0
blast CAE68255 Hypothetical protein CBG13932 [Caenorhabditis briggsae] 0.0
blast AAC02578 Hypothetical protein F53H1.4a [Caenorhabditis elegans] ref|NP_001023217.1| F53H1.4a [Caenorhabditis elegans] 0.0
blast CAE68256 Hypothetical protein CBG13933 [Caenorhabditis briggsae] 0.0
blast XP_783119 PREDICTED: similar to putative protein, with at least 2 transmembrane domains, of eukaryotic origin (63.2 kD) (4C103) [Strongylocentrotus purpuratus] 1.0E-125
blast AAC02577 Hypothetical protein F53H1.3 [Caenorhabditis elegans] ref|NP_500065.2| F53H1.3 [Caenorhabditis elegans] 1.0E-117
blast CAE68255 Hypothetical protein CBG13932 [Caenorhabditis briggsae] 1.0E-107
Sequence
Target Sequence 
>C. ELEGANS:183686_AT
tgtccgtccagatttcactacatcaattgtacagcacgtccatcatttgccactgctctg
ggagcttgtactgctcggcgagccccttctcatcgttgctccgacgccatccatcacttc
tgctttagttcagtcaatcgttggattactgtcacctctgaggattgtcaacgattttcg
accgtattttacgattcatgattcggaatttcgagaatattcgtcgaaaagtcgatcgcc
accccgtgtgattcttggcgtaacgaatccgttcttcatcaaagccctggaccattggcc
gcatatacttaaagtcgcagagcaaggagctgaaattggtggagacgatatgaagaagcc
gaaaaaaacgtggtcatcctcccgaactctggacaccccagccggcctatacactcaata
caagccatttctctcgaaagataaatccctcgcaaagaaattactccgatccggaaactc
gttggaggtacagtaccccgctacctacagtaccccttcctactcgtcacttcttggagt
t

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTCCGTCCAGATTTCACTACATCA 624 557 648 Antisense
TTGTACAGCACGTCCATCATTTGCC 175 707 674 Antisense
GCTCTGGGAGCTTGTACTGCTCGGC 707 299 702 Antisense
AATCGTTGGATTACTGTCACCTCTG 159 169 783 Antisense
GATTGTCAACGATTTTCGACCGTAT 571 433 810 Antisense
TTCGTCGAAAAGTCGATCGCCACCC 384 695 867 Antisense
CCCCGTGTGATTCTTGGCGTAACGA 50 261 889 Antisense
ATCCGTTCTTCATCAAAGCCCTGGA 367 35 914 Antisense
CTGGACCATTGGCCGCATATACTTA 428 241 934 Antisense
CACTCAATACAAGCCATTTCTCTCG 452 197 1059 Antisense
TTCCTACTCGTCACTTCTTGGAGTT 381 699 1164 Antisense
Sequence Source Affymetrix Proprietary Database