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[183674_at]

Description C17C3.8 /REP_DB=WormBase Gene ID /WP=CE04028 /TR=Q18053 /GB=AAK31422.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 60      4grp_total > 13      6grp_cgc > 95      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 38.5
(-0.6)
A 		2.9
(-3)
A 		10.8
(-2.5)
A 		91.8
(3)
A 		89
B 65.5
(3)
A 		32.8
(-0.4)
A 		8.6
(-3)
A 		41.3
(0.4)
A 		57
C 42.4
(-2.2)
A 		33.2
(-3)
A 		33.6
(-3)
A 		101.1
(3)
P 		68
avr 48.8
(0.1) 		23
(-2.5) 		17.7
(-3) 		78.1
(3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 102
1 2 3 4 5 6 range
A 51.1
(1.6)
A 		9.6
(-2.7)
A 		64.2
(3)
A 		7
(-3)
A 		10.6
(-2.6)
A 		32.7
(-0.3)
A 		57
B 60.1
(3)
A 		6.5
(-3)
A 		7.8
(-2.9)
A 		32
(-0.1)
A 		23.4
(-1.1)
A 		30.7
(-0.3)
A 		54
C 5.7
(-3)
A 		60
(0.2)
A 		7.2
(-2.9)
A 		107.1
(3)
A 		5.3
(-3)
A 		18.6
(-2.2)
A 		102
avr 39
(1.4) 		25.4
(-0.9) 		26.4
(-0.8) 		48.7
(3) 		13.1
(-3) 		27.3
(-0.6) 		36
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 14
(3)
A 		0.6
(-3)
A 		2.8
(-2)
A 		13.4
(2.7)
A 		13
B 20.9
(3)
A 		8.1
(-1.1)
A 		2
(-3)
A 		8.8
(-0.8)
A 		19
C 14
(0.6)
A 		9.6
(-3)
A 		11.7
(-1.3)
A 		17
(3)
P 		7
avr 16.3
(3) 		6.1
(-2.7) 		5.5
(-3) 		13.1
(1.2) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 9.4
(0.7)
A 		3.4
(-2.2)
A 		14
(3)
A 		1.8
(-3)
A 		3.2
(-2.3)
A 		8
(0)
A 		12
B 20.9
(3)
A 		2.2
(-2.9)
A 		1.9
(-3)
A 		8.5
(-0.9)
A 		9.5
(-0.6)
A 		11.5
(0)
A 		19
C 2.1
(-2.9)
A 		18.3
(2)
A 		1.8
(-3)
A 		21.5
(3)
A 		1.7
(-3)
A 		6.2
(-1.6)
A 		20
avr 10.8
(3) 		8
(0.2) 		5.9
(-1.9) 		10.6
(2.8) 		4.8
(-3) 		8.6
(0.8) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183674_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5966
Sequence Type Exemplar sequence
Representative Public ID C17C3.8 NCBI
Target Description C17C3.8 /REP_DB=WormBase Gene ID /WP=CE04028 /TR=Q18053 /GB=AAK31422.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062661(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062661 NCBI Caenorhabditis elegans Helix Loop Helix family member (hlh-26) (hlh-26) mRNA, complete cds. 11/11 None
SNAP00000005283 ENSEMBL cdna:SNAP chromosome:CEL140:II:5532949:5533692:-1 11/11 None
GENEFINDER00000005300 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5531368:5533692:-1 11/11 None
C17C3.8 ENSEMBL cdna:known chromosome:CEL140:II:5532827:5533692:-1 gene:C17C3.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5532946-5533690(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hlh-26
Ensembl C17C3.8
Entrez Gene 173939 Entrez gene
SwissProt Q18053 EMBL-EBI
WormBase CE04028 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK31422 Helix loop helix protein 26 [Caenorhabditis elegans] ref|NP_495062.1| Helix Loop Helix family member (hlh-26) [Caenorhabditis elegans] 6.0E-82
blast AAK31422 Helix loop helix protein 26 [Caenorhabditis elegans] ref|NP_495062.1| Helix Loop Helix family member (hlh-26) [Caenorhabditis elegans] 2.0E-80
blast AAK31425 Hypothetical protein C17C3.9 [Caenorhabditis elegans] ref|NP_495061.2| C17C3.9 [Caenorhabditis elegans] 2.0E-79
blast AAK31423 Helix loop helix protein 27 [Caenorhabditis elegans] ref|NP_495065.1| Helix Loop Helix family member (hlh-27) [Caenorhabditis elegans] 5.0E-13
blast AAK31421 Helix loop helix protein 25 [Caenorhabditis elegans] ref|NP_495063.1| Helix Loop Helix family member (hlh-25) [Caenorhabditis elegans] 5.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR001092 EMBL-EBI Basic helix-loop-helix dimerization domain bHLH 2.8E-8
Sequence
Target Sequence 
>C. ELEGANS:183674_AT
tccaacttcgtcatcatctgggtccccttcttcacatggacatcgatctgaaacagaaaa
acaaagaagagatgatactaatgatctgttgaatgaattcaagaaaatcgttcaaaaatc
ggaatctgaaaaactttcaaaagaagaggttctatttcgtattgtgaaattgttatctgg
tattcaacttcatcacgaatcgttttcaacatcgcctggaccaattagaagtattaaaaa
gattaaaagcgatcgggaacaagttcgccgaaataagagagttgccgcgtacagagagct
cagaaaatttattgcattgaataatttgagtagctcagaagaaatagacaaaatggagaa
tttgaaggttctagaaatcatctttgaagtgattcgtggaaagtcgatcacatgtacagt
accttgcctaccatttccaatacttccttttctcccagtattcccagtttattcgccatt
agttttcaattcatacaatcaattttcactttatcctcctcacatgcctacagttcagat
tcctattgtcccaagttccagcttcgaaattgacgctct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCAACTTCGTCATCATCTGGGTCC 535 587 24 Antisense
TCCCCTTCTTCACATGGACATCGAT 243 597 46 Antisense
TATCTGGTATTCAACTTCATCACGA 520 659 197 Antisense
CGAATCGTTTTCAACATCGCCTGGA 608 253 219 Antisense
AGTTGCCGCGTACAGAGAGCTCAGA 424 63 303 Antisense
CACATGTACAGTACCTTGCCTACCA 315 195 432 Antisense
TCCCAGTTTATTCGCCATTAGTTTT 609 597 485 Antisense
CAATCAATTTTCACTTTATCCTCCT 313 181 519 Antisense
TCCTCACATGCCTACAGTTCAGATT 295 591 540 Antisense
AGTTCAGATTCCTATTGTCCCAAGT 16 65 555 Antisense
GTTCCAGCTTCGAAATTGACGCTCT 347 441 578 Antisense
Sequence Source Affymetrix Proprietary Database