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[183650_at]

Description F28F5.1 /REP_DB=WormBase Gene ID /WP=CE00716 /TR=Q19888 /GB=AAA50681.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 66      4grp_total > 63      6grp_cgc > 57      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 30.4
(-0.2)
A 		9.2
(-3)
A 		55.1
(3)
A 		10.1
(-2.9)
A 		46
B 10.5
(-2.2)
A 		9.8
(-3)
A 		14.9
(3)
A 		11.7
(-0.8)
A 		5
C 34.6
(3)
A 		17.2
(-1.4)
A 		18.8
(-1)
A 		10.6
(-3)
A 		24
avr 25.2
(1.6) 		12.1
(-2.6) 		29.6
(3) 		10.8
(-3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 8.3
(-2.9)
A 		16.8
(-0.3)
A 		8
(-3)
A 		12.1
(-1.7)
A 		20.3
(0.8)
A 		27.3
(3)
A 		19
B 9.8
(-0.9)
A 		12.6
(-0)
A 		19
(2)
A 		22
(3)
A 		3.3
(-3)
A 		7.3
(-1.7)
A 		19
C 6.9
(-3)
A 		13.5
(-0.6)
A 		10.8
(-1.6)
A 		8
(-2.6)
A 		6.9
(-3)
A 		23.1
(3)
A 		16
avr 8.3
(-3) 		14.3
(0.3) 		12.6
(-0.6) 		14
(0.1) 		10.2
(-2) 		19.2
(3) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 11.1
(1.5)
A 		2
(-2.8)
A 		14.4
(3)
A 		1.5
(-3)
A 		13
B 3.3
(1.9)
A 		2.4
(-3)
A 		3.5
(3)
A 		2.5
(-2.5)
A 		1
C 11.4
(3)
A 		4.9
(-1.1)
A 		6.5
(-0.1)
A 		1.8
(-3)
A 		10
avr 8.6
(3) 		3.1
(-1.9) 		8.1
(2.6) 		1.9
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 1.5
(-3)
A 		6
(2.3)
A 		1.7
(-2.8)
A 		3.1
(-1.1)
A 		6.1
(2.4)
A 		6.6
(3)
A 		5
B 3.4
(-0.3)
A 		4.2
(0.8)
A 		4.6
(1.4)
A 		5.8
(3)
A 		1.4
(-3)
A 		2.7
(-1.2)
A 		4
C 2.6
(-2)
A 		4.1
(-0.5)
A 		2.7
(-1.9)
A 		1.6
(-3)
A 		2.2
(-2.4)
A 		7.7
(3)
A 		6
avr 2.5
(-3) 		4.8
(1.3) 		3
(-2.1) 		3.5
(-1.1) 		3.2
(-1.7) 		5.7
(3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183650_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6654
Sequence Type Exemplar sequence
Representative Public ID F28F5.1 NCBI
Target Description F28F5.1 /REP_DB=WormBase Gene ID /WP=CE00716 /TR=Q19888 /GB=AAA50681.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027552(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027552 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-152) (tag-152) mRNA, complete cds. 11/11 None
GENEFINDER00000021166 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6823262:6834232:1 11/11 None
SNAP00000021163 ENSEMBL cdna:SNAP chromosome:CEL140:III:6830038:6831391:1 11/11 None
F28F5.1 ENSEMBL cdna:known chromosome:CEL140:III:6829100:6831391:1 gene:F28F5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175953 3 1 NM_001027552 174424_at (11), 183650_at (11)
NM_066086 174424_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6829098-6831390(+) 100.0 100.0
Public Domain and Genome References
Ensembl F28F5.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001022723 Temporarily Assigned Gene name family member (tag-152) [Caenorhabditis elegans] 0.0
blast BAD91086.1 Ten-1L [Caenorhabditis elegans] 0.0
blast NP_741203 Temporarily Assigned Gene name family member (tag-152) [Caenorhabditis elegans] gb|AAC47044.3| Temporarily assigned gene name protein 152, isoform a [Caenorhabditis elegans] 0.0
blast T16207 hypothetical protein F28F5.1 - Caenorhabditis elegans 3.0E-71
blast T16207 hypothetical protein F28F5.1 - Caenorhabditis elegans 3.0E-63
blast NP_001022723 Temporarily Assigned Gene name family member (tag-152) [Caenorhabditis elegans] 2.0E-61
blast NP_001022723 Temporarily Assigned Gene name family member (tag-152) [Caenorhabditis elegans] 7.0E-61
blast NP_001022723 Temporarily Assigned Gene name family member (tag-152) [Caenorhabditis elegans] 2.0E-60
blast T16207 hypothetical protein F28F5.1 - Caenorhabditis elegans 5.0E-58
blast BAD91086.1 Ten-1L [Caenorhabditis elegans] 2.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR006209 EMBL-EBI EGF-like domain 3.8E-5
Pfam IPR001258 EMBL-EBI NHL repeat 3.2
Pfam IPR001258 EMBL-EBI NHL repeat 0.033
Pfam IPR001258 EMBL-EBI NHL repeat 2.8
Pfam IPR001258 EMBL-EBI NHL repeat 0.023
Pfam IPR001258 EMBL-EBI NHL repeat 9.8E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022723.1 1 259-281
Sequence
Target Sequence 
>C. ELEGANS:183650_AT
ccgttacctcatccatcagagtgcgggcagccgtgcgtttcgggacagtcacaaatgttc
cagcacaggaccacgaatgcgcaaggcccaccgcctaatcgccctatgccacggccaccg
gcaggaatgccgatgatgacgtcatcacacgaacatgactacacaaatgattatgaggat
ccggaggaaatggcaagaagtcgtggagaaggattttcaaaccatttgctcatcaaaacg
acaccgccaccacaacctcaccccaacttcaactcctatgaaatgtccatgtcacaacag
cgtcgttcccagcaacaccagcagccaatggctccaccactttcagattgttgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGTTACCTCATCCATCAGAGTGCG 578 263 61 Antisense
TCCATCAGAGTGCGGGCAGCCGTGC 19 579 72 Antisense
CCGTGCGTTTCGGGACAGTCACAAA 246 265 91 Antisense
GACAGTCACAAATGTTCCAGCACAG 237 369 104 Antisense
CACAGGACCACGAATGCGCAAGGCC 522 193 124 Antisense
CCACGGCCACCGGCAGGAATGCCGA 424 273 169 Antisense
GGCAGGAATGCCGATGATGACGTCA 645 535 180 Antisense
GGATTTTCAAACCATTTGCTCATCA 618 509 271 Antisense
ACCCCAACTTCAACTCCTATGAAAT 385 89 320 Antisense
AATGTCCATGTCACAACAGCGTCGT 66 163 342 Antisense
GGCTCCACCACTTTCAGATTGTTGG 90 541 390 Antisense
Sequence Source Affymetrix Proprietary Database