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[183646_at]

Description R10H10.4 /REP_DB=WormBase Gene ID /WP=CE06295 /TR=Q21914 /GB=CAA94608.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 04      4grp_highest_8_8 > 63      4grp_high_8_8 > 19      6grp_highest_nonorm > 50      6grp_total > 14      4grp_total > 29      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 372
1 2 3 4 range
A 709.8
(3)
P 		562.4
(-0.5)
P 		453.8
(-3)
P 		598.6
(0.4)
P 		256
B 586.3
(-3)
P 		826
(3)
P 		797.7
(2.3)
P 		724.4
(0.5)
P 		240
C 768.2
(2.4)
P 		590.6
(-0.8)
P 		467.4
(-3)
P 		804.7
(3)
P 		337
avr 688.1
(2.1) 		659.7
(0.8) 		573
(-3) 		709.2
(3) 		136

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 859
1 2 3 4 5 6 range
A 642.1
(3)
P 		633.5
(2.9)
P 		645.3
(3)
P 		428.4
(0.3)
P 		300.6
(-1.2)
P 		156.8
(-3)
P 		489
B 538.3
(1)
P 		693.5
(3)
P 		556.5
(1.3)
P 		397.5
(-0.8)
P 		459.4
(0)
P 		223.4
(-3)
P 		470
C 1016.2
(3)
P 		579.2
(-0.6)
P 		325.3
(-2.7)
P 		478.7
(-1.4)
P 		344.6
(-2.5)
P 		285.7
(-3)
P 		731
avr 732.2
(3) 		635.4
(1.9) 		509
(0.4) 		434.9
(-0.5) 		368.2
(-1.3) 		222
(-3) 		510
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 171
1 2 3 4 range
A 258.5
(3)
P 		122.1
(-1.8)
P 		118.6
(-1.9)
P 		87.6
(-3)
P 		171
B 187
(0.9)
P 		204
(3)
P 		184.8
(0.7)
P 		154.4
(-3)
P 		50
C 254.3
(3)
P 		169.8
(-1.3)
P 		162.9
(-1.6)
P 		135.7
(-3)
P 		119
avr 233.3
(3) 		165.3
(-0.8) 		155.4
(-1.4) 		125.9
(-3) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 338
1 2 3 4 5 6 range
A 118.4
(-0.4)
P 		225.3
(3)
P 		140.5
(0.3)
P 		108.6
(-0.7)
P 		90.7
(-1.3)
P 		38.2
(-3)
P 		187
B 187.3
(1.2)
P 		230.8
(3)
P 		135.1
(-0.9)
P 		105.3
(-2.1)
P 		186.7
(1.2)
P 		83.3
(-3)
P 		148
C 376.6
(3)
P 		176.7
(-1.1)
P 		82.8
(-3)
P 		96.3
(-2.7)
P 		111.5
(-2.4)
P 		95.6
(-2.7)
P 		294
avr 227.4
(3) 		210.9
(2.4) 		119.5
(-1.2) 		103.4
(-1.8) 		129.6
(-0.8) 		72.4
(-3) 		155
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183646_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10279
Sequence Type Exemplar sequence
Representative Public ID R10H10.4 NCBI
Target Description R10H10.4 /REP_DB=WormBase Gene ID /WP=CE06295 /TR=Q21914 /GB=CAA94608.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069519(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069519 NCBI Caenorhabditis elegans R10H10.4 (R10H10.4) mRNA, complete cds. 11/11 None
SNAP00000020333 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10394635:10394926:-1 11/11 None
GENEFINDER00000020341 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10394635:10394926:-1 11/11 None
R10H10.4 ENSEMBL cdna:known chromosome:CEL140:IV:10394635:10394926:-1 gene:R10H10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10394640-10394932(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R10H10.4
Entrez Gene 187788 Entrez gene
SwissProt Q21914 EMBL-EBI
WormBase CE06295 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94608 Hypothetical protein R10H10.4 [Caenorhabditis elegans] ref|NP_501920.1| R10H10.4 [Caenorhabditis elegans] 2.0E-39
blast CAE63827 Hypothetical protein CBG08380 [Caenorhabditis briggsae] 2.0E-35
blast AAB00576 Hypothetical protein T01C8.2 [Caenorhabditis elegans] ref|NP_510707.1| T01C8.2 [Caenorhabditis elegans] 8.0E-13
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501920.1 1 15-37
R10H10.4 1 15-37
SNAP00000020333 1 15-37
GENEFINDER00000020341 1 15-37
Sequence
Target Sequence 
>C. ELEGANS:183646_AT
aacagttcgaagtacttgactacgcagcaccactcttcgttggagcagcttttgcagtga
ttgtattcatgctaacattcgttatcaactttgccttcatcagaagaaccgatgaagtca
ctgcttttgaaaagctaggagcaaagtacaatttgcgagttggaccacaccgtgtctctc
tggtcaaggaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACAGTTCGAAGTACTTGACTACGC 327 135 23 Antisense
CGAAGTACTTGACTACGCAGCACCA 210 253 30 Antisense
ACCACTCTTCGTTGGAGCAGCTTTT 539 85 51 Antisense
AGCAGCTTTTGCAGTGATTGTATTC 492 77 66 Antisense
GATTGTATTCATGCTAACATTCGTT 263 435 81 Antisense
AACATTCGTTATCAACTTTGCCTTC 5 137 96 Antisense
GTTATCAACTTTGCCTTCATCAGAA 506 447 103 Antisense
CTTTGCCTTCATCAGAAGAACCGAT 249 223 111 Antisense
AGAACCGATGAAGTCACTGCTTTTG 670 73 127 Antisense
ATTTGCGAGTTGGACCACACCGTGT 316 17 173 Antisense
CACCGTGTCTCTCTGGTCAAGGAGA 400 201 190 Antisense
Sequence Source Affymetrix Proprietary Database