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[183643_at]

Description Y50E8A.6 /REP_DB=WormBase Gene ID /WP=CE22308 /TR=Q9NAF2 /GB=CAB55049.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 33      4grp_total > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 14.9
(-3)
A 		14.8
(-3)
A 		34.2
(-1.6)
A 		95.2
(3)
A 		80
B 41.5
(3)
A 		13.2
(-3)
A 		15.7
(-2.4)
A 		13
(-3)
A 		29
C 12.4
(-2.7)
A 		14.8
(-2.4)
A 		9.4
(-3)
A 		68.2
(3)
A 		59
avr 22.9
(-1.8) 		14.3
(-3) 		19.8
(-2.3) 		58.8
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 12.7
(-2)
A 		10.4
(-2.3)
A 		56.4
(3)
A 		29.3
(-0.1)
A 		44.3
(1.6)
A 		4.4
(-3)
A 		52
B 62.4
(3)
A 		17.2
(-2.2)
A 		11.9
(-2.8)
A 		23.6
(-1.5)
A 		10.4
(-3)
A 		12.7
(-2.7)
A 		52
C 27.3
(1.5)
A 		25.3
(1.1)
A 		35.4
(3)
A 		14.6
(-1)
A 		3.9
(-3)
A 		8.5
(-2.1)
A 		32
avr 34.1
(2.9) 		17.6
(-0.9) 		34.6
(3) 		22.5
(0.2) 		19.5
(-0.5) 		8.5
(-3) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 5.4
(-1.8)
A 		3.2
(-3)
A 		8.9
(0.2)
A 		13.9
(3)
A 		11
B 13.2
(3)
A 		3.3
(-2.7)
A 		3.6
(-2.5)
A 		2.8
(-3)
A 		10
C 4.1
(-2.4)
A 		4.3
(-2.3)
A 		3.3
(-3)
A 		11.5
(3)
A 		8
avr 7.6
(1.1) 		3.6
(-3) 		5.3
(-1.2) 		9.4
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 2.3
(-2.4)
A 		3.7
(-1.7)
A 		12.3
(2.5)
A 		7.4
(0.1)
A 		13.4
(3)
A 		1.1
(-3)
A 		12
B 21.7
(3)
A 		5.7
(-2.1)
A 		2.9
(-3)
A 		6.2
(-1.9)
A 		4.2
(-2.6)
A 		4.7
(-2.4)
A 		19
C 10.1
(3)
A 		7.7
(1.4)
A 		9
(2.3)
A 		2.9
(-1.9)
A 		1.3
(-3)
A 		2.9
(-1.9)
A 		9
avr 11.4
(3) 		5.7
(-1) 		8.1
(0.7) 		5.5
(-1.2) 		6.3
(-0.6) 		2.9
(-3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183643_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14730
Sequence Type Exemplar sequence
Representative Public ID Y50E8A.6 NCBI
Target Description Y50E8A.6 /REP_DB=WormBase Gene ID /WP=CE22308 /TR=Q9NAF2 /GB=CAB55049.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074239(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074239 NCBI Caenorhabditis elegans Y50E8A.6 (Y50E8A.6) mRNA, complete cds. 11/11 None
SNAP00000008981 ENSEMBL cdna:SNAP chromosome:CEL140:V:14741171:14743915:-1 11/11 None
GENEFINDER00000009973 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14741171:14743915:-1 11/11 None
Y50E8A.6 ENSEMBL cdna:known chromosome:CEL140:V:14741171:14743915:-1 gene:Y50E8A.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14741170-14743915(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50E8A.6
Entrez Gene 190107 Entrez gene
SwissProt Q9NAF2 EMBL-EBI
WormBase CE22308 Wormbase
Functional Annotations
Ortholog
ID Title Organism
YEAST_2:1777556_AT Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6072 glycerol-3-phosphate metabolism inferred from electronic annotation QuickGO AmiGO
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
9331 glycerol-3-phosphate dehydrogenase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4368 glycerol-3-phosphate dehydrogenase activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB55049 Hypothetical protein Y50E8A.6 [Caenorhabditis elegans] ref|NP_506640.1| Y50E8A.6 [Caenorhabditis elegans] 0.0
blast CAE60843 Hypothetical protein CBG04552 [Caenorhabditis briggsae] 0.0
blast CAE66579 Hypothetical protein CBG11896 [Caenorhabditis briggsae] 1.0E-165
Protein Domains
Database ID Description E-Value
Pfam IPR006076 EMBL-EBI FAD dependent oxidoreductase 4.1E-89
Sequence
Target Sequence 
>C. ELEGANS:183643_AT
tatacttcaatgctctatcggaccatatctcaaaactatggaattgaagaacaagtcgcc
actcatttatgccaaacgtatggggataaagtctatgaagtactcaaactatgcaagtca
actggccagaaattcccagtcatcggacatcggctccatccagattttccatttttggag
gcagaagtaagatatgcagtgaaggaatatgcacggattccagctgatattctcgcacgt
cgtacacgcctttctttgttggatgctcgagctgcaagacaagttttgccacgtatcgtt
gcaataatggcggaggagttgaaatggagtccgagtgaacaaattttttattttgatgaa
ggaatgaaatttttgaaagtggaaaatgggagtgttcaatcttggaaaaaagagcatagt
ggagttgaattgcaatcattagaagatcagaacgtcaatattccacaggatgtactcgac
gatgttgttatggaagcaaatgaagggaataaatatgtatcgctggaagatttacaaaaa
atttttatggatagcaaaaacggttgtacgaaaggacgtcgcctgattgaccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATACTTCAATGCTCTATCGGACCA 635 659 1405 Antisense
GCTCTATCGGACCATATCTCAAAAC 244 301 1416 Antisense
GAACAAGTCGCCACTCATTTATGCC 350 353 1453 Antisense
GCAAGTCAACTGGCCAGAAATTCCC 487 321 1517 Antisense
AAATTCCCAGTCATCGGACATCGGC 506 113 1534 Antisense
AATATGCACGGATTCCAGCTGATAT 585 171 1610 Antisense
TACACGCCTTTCTTTGTTGGATGCT 65 643 1647 Antisense
GTTGGATGCTCGAGCTGCAAGACAA 6 437 1662 Antisense
GACAAGTTTTGCCACGTATCGTTGC 270 365 1682 Antisense
TATTCCACAGGATGTACTCGACGAT 490 645 1863 Antisense
GAAAGGACGTCGCCTGATTGACCAC 493 361 1974 Antisense
Sequence Source Affymetrix Proprietary Database