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[183599_at]

Description C48E7.5 /REP_DB=WormBase Gene ID /WP=CE08820 /TR=O02078 /GB=AAK21359.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 30      4grp_total > 53      6grp_cgc > 72      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 180
1 2 3 4 range
A 188.4
(3)
P 		106
(-0.8)
P 		72.8
(-2.3)
M 		57.2
(-3)
A 		131
B 172.6
(3)
P 		144.9
(2)
P 		136.7
(1.7)
P 		8
(-3)
A 		165
C 155.8
(3)
P 		155.4
(3)
P 		100.2
(-0.2)
P 		49.9
(-3)
A 		106
avr 172.3
(3) 		135.4
(1.3) 		103.2
(-0.1) 		38.4
(-3) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 205
1 2 3 4 5 6 range
A 129.7
(-0.6)
A 		228.7
(3)
P 		168.4
(0.8)
P 		204.2
(2.1)
P 		99.8
(-1.6)
A 		61.6
(-3)
A 		167
B 86.8
(-1.7)
A 		152.1
(0.9)
P 		206
(3)
P 		126.9
(-0.1)
M 		104.7
(-1)
A 		52.7
(-3)
A 		153
C 154.6
(1.4)
P 		201.3
(3)
P 		176.3
(2.2)
P 		145.8
(1.1)
P 		83.6
(-1)
P 		23.8
(-3)
A 		178
avr 123.7
(0.2) 		194
(3) 		183.6
(2.6) 		159
(1.6) 		96
(-1) 		46
(-3) 		148
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 68.6
(3)
P 		23
(-1.5)
P 		19
(-1.9)
M 		8.4
(-3)
A 		60
B 55.1
(3)
P 		35.8
(0.8)
P 		31.7
(0.4)
P 		1.7
(-3)
A 		53
C 51.6
(3)
P 		44.7
(2)
P 		34.9
(0.7)
P 		8.4
(-3)
A 		43
avr 58.4
(3) 		34.5
(0.3) 		28.5
(-0.4) 		6.2
(-3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 23.9
(-2.2)
A 		81.3
(3)
P 		36.7
(-1)
P 		51.8
(0.3)
P 		30.1
(-1.6)
A 		15
(-3)
A 		66
B 30.2
(-1)
A 		50.6
(3)
P 		50
(2.9)
P 		33.6
(-0.3)
M 		42.5
(1.4)
A 		19.7
(-3)
A 		31
C 57.3
(2.5)
P 		61.4
(3)
P 		44.9
(1.1)
P 		29.3
(-0.6)
P 		27.1
(-0.9)
P 		8
(-3)
A 		53
avr 37.1
(-0.3) 		64.4
(3) 		43.9
(0.5) 		38.2
(-0.1) 		33.2
(-0.7) 		14.2
(-3) 		50
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183599_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.886
Sequence Type Exemplar sequence
Representative Public ID C48E7.5 NCBI
Target Description C48E7.5 /REP_DB=WormBase Gene ID /WP=CE08820 /TR=O02078 /GB=AAK21359.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059403(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059403 NCBI Caenorhabditis elegans COlmedin (collagen plus olFactomedin) family member (cof-2) (cof-2) mRNA, complete cds. 9/11 None
GENEFINDER00000002353 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6258706:6261613:1 9/11 None
C48E7.5 ENSEMBL cdna:known chromosome:CEL140:I:6258706:6260956:1 gene:C48E7.5 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000002341 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6258359-6259605(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol cof-2
Ensembl C48E7.5
Entrez Gene 183582 Entrez gene
SwissProt Q8IG71 EMBL-EBI
WormBase CE36279 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1595073_AT similar to collomin cfa
CANINE:1591151_AT similar to collomin cfa
CANINE_2:CFAAFFX.23602.1.S1_S_AT similar to collomin cfa
CHICKEN:GGAAFFX.8464.1.S1_AT similar to VDLC9339 gga
HU35KSUBB:RC_H04768_AT gliomedin hs
U133_X3P:HS.30484.0.A1_3P_AT gliomedin hs
HG-U133_PLUS_2:230360_AT gliomedin hs
HG-U133B:230360_AT gliomedin hs
HG-U95C:64210_AT gliomedin hs
HU35KSUBC:RC_N51935_AT Gliomedin hs
U133_X3P:HS.47374.0.S1_3P_AT Gliomedin hs
HG-U133_PLUS_2:232649_AT Gliomedin hs
HG-U133B:232649_AT Gliomedin hs
HG-U95B:54371_AT Gliomedin hs
HG-U95B:45090_AT Gliomedin hs
HU35KSUBC:RC_R27788_AT Gliomedin hs
Protein Similarities
Method ID Description E-Value
blast AAN60526 Colmedin (collagen plus olfactomedin) family protein 2 [Caenorhabditis elegans] ref|NP_491804.2| COlmedin (collagen plus olFactomedin) family member (cof-2) [Caenorhabditis elegans] gb|AAR87492.1| collagen/olfactomedin domain containing protein 2 [Caenorhabditis elegans] 0.0
blast T15173 hypothetical protein C48E7.4 - Caenorhabditis elegans 1.0E-125
blast CAE67148 Hypothetical protein CBG12573 [Caenorhabditis briggsae] 1.0E-122
blast T15173 hypothetical protein C48E7.4 - Caenorhabditis elegans 1.0E-116
blast CAE67148 Hypothetical protein CBG12573 [Caenorhabditis briggsae] 1.0E-113
Protein Domains
Database ID Description E-Value
Pfam IPR003112 EMBL-EBI Olfactomedin-like 1.7E-34
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.3E-5
Pfam IPR003112 EMBL-EBI Olfactomedin-like 4.6E-33
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491804.2 1 7-29
C48E7.5 1 7-29
GENEFINDER00000002353 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:183599_AT
gttccaaacgtgatattcccctatcaagaggcaaaaatgcttcagacatcctgtggctaa
catccttgtcttctgtaaaaattggagagctaatgcacaaatgtactgaaattcatgaat
actgtagtgaacaagacgacgtgcgcgggaaaccgggccctgttggacctgtaggcccac
ctggaaagagtggacctgctggaccacaaggacgaagagggttaatgggaatcccagggc
accctggacctgtgggaccacctggaaatattggaaaagatgcggattgttcttcatgcc
cgattagagatgagctgttgatggaaagggagttcaaatgtcctactattgaaaatctgg
aatgcccgaacagagaagccattccaacaactccctggccttcagtcacacatcaaccaa
tgcctggattggttcagcaggtgacttcagtaataactttcagatccaaagcatattttc
agatcctttccaactacagtgaagtcgagggatgcgtgaaaatatgtttgaaaaactaca
ctctcgatgaagaaattgaagagaccattacgactccagttgcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCCAAACGTGATATTCCCCTATC 169 441 215 Antisense
ATGCTTCAGACATCCTGTGGCTAAC 583 41 251 Antisense
GCTAACATCCTTGTCTTCTGTAAAA 698 301 270 Antisense
ACCTGTAGGCCCACCTGGAAAGAGT 377 1 381 Antisense
TAATGGGAATCCCAGGGCACCCTGG 584 627 437 Antisense
TGGACCTGTGGGACCACCTGGAAAT 178 547 459 Antisense
GATGCGGATTGTTCTTCATGCCCGA 166 415 493 Antisense
CAGTCACACATCAACCAATGCCTGG 633 207 617 Antisense
GGATTGGTTCAGCAGGTGACTTCAG 289 509 640 Antisense
TTTTCAGATCCTTTCCAACTACAGT 172 677 690 Antisense
GAGACCATTACGACTCCAGTTGCAT 224 395 775 Antisense
Sequence Source Affymetrix Proprietary Database