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[183578_at]

Description M01G5.3 /REP_DB=WormBase Gene ID /WP=CE19537 /TR=O76687 /GB=AAC26943.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 71      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 64      6grp_total > 61      4grp_total > 62      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 509
1 2 3 4 range
A 1013.5
(3)
P 		786
(-0.4)
P 		914.9
(1.5)
P 		615.2
(-3)
P 		398
B 1077.1
(3)
P 		809.5
(-0.2)
P 		806.6
(-0.2)
P 		568
(-3)
P 		509
C 968.1
(3)
P 		847
(1.2)
P 		803.3
(0.5)
P 		567.8
(-3)
P 		400
avr 1019.6
(3) 		814.2
(0.2) 		841.6
(0.5) 		583.7
(-3) 		436

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 465
1 2 3 4 5 6 range
A 575.5
(-3)
P 		742.4
(-0.8)
P 		714.2
(-1.1)
P 		1024.5
(3)
P 		655.2
(-1.9)
P 		847.4
(0.6)
P 		449
B 579.4
(-2.6)
P 		704.9
(0.2)
P 		786
(2)
P 		742.6
(1.1)
P 		830.5
(3)
P 		559.2
(-3)
P 		271
C 974
(3)
P 		595.9
(-3)
P 		949.6
(2.6)
P 		737.8
(-0.7)
P 		811.5
(0.4)
P 		607.4
(-2.8)
P 		378
avr 709.6
(-1.6) 		681.1
(-2.6) 		816.6
(2.3) 		835
(3) 		765.7
(0.5) 		671.3
(-3) 		164
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 279
1 2 3 4 range
A 369
(3)
P 		170.6
(-1.3)
P 		239.2
(0.2)
P 		90.1
(-3)
P 		279
B 343.5
(3)
P 		199.9
(-0.9)
P 		186.9
(-1.2)
P 		121
(-3)
P 		223
C 320.5
(3)
P 		243.5
(0.9)
P 		280
(1.9)
P 		95.7
(-3)
P 		225
avr 344.3
(3) 		204.7
(-0.5) 		235.4
(0.3) 		102.3
(-3) 		242

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 255
1 2 3 4 5 6 range
A 106.1
(-3)
P 		264
(3)
P 		155.5
(-1.1)
P 		259.8
(2.8)
P 		197.7
(0.5)
P 		206.3
(0.8)
P 		158
B 201.6
(-2.6)
P 		234.6
(-1.2)
P 		190.9
(-3)
P 		196.7
(-2.8)
P 		337.4
(3)
P 		208.5
(-2.3)
P 		147
C 361
(3)
P 		181.8
(-2.1)
P 		241.7
(-0.4)
P 		148.4
(-3)
P 		262.6
(0.2)
P 		203.3
(-1.5)
P 		213
avr 222.9
(-0.7) 		226.8
(-0.4) 		196
(-3) 		201.6
(-2.5) 		265.9
(3) 		206
(-2.1) 		70
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183578_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8117
Sequence Type Exemplar sequence
Representative Public ID M01G5.3 NCBI
Target Description M01G5.3 /REP_DB=WormBase Gene ID /WP=CE19537 /TR=O76687 /GB=AAC26943.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065013(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065013 NCBI Caenorhabditis elegans M01G5.3 (M01G5.3) mRNA, complete cds. 11/11 None
GENEFINDER00000031213 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1509397:1517983:-1 11/11 None
M01G5.3 ENSEMBL cdna:known chromosome:CEL140:III:1509397:1517983:-1 gene:M01G5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1509484-1512949(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M01G5.3
Entrez Gene 175305 Entrez gene
SwissProt O76687 EMBL-EBI
WormBase CE29983 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:261566_AT expressed protein at
CANINE_2:CFAAFFX.13060.1.S1_AT similar to transmembrane protein induced by tumor necrosis factor alpha cfa
CHICKEN:GGAAFFX.12498.1.S1_S_AT similar to LOC330189 protein gga
HG-U133B:236667_AT hypothetical LOC144404 hs
HG-U133_PLUS_2:236667_AT hypothetical LOC144404 hs
HU35KSUBA:HUM_ALU_AT hypothetical LOC144404 hs
HU35KSUBB:HUM_ALU_AT hypothetical LOC144404 hs
HU35KSUBC:HUM_ALU_AT hypothetical LOC144404 hs
HU35KSUBD:HUM_ALU_AT hypothetical LOC144404 hs
HUGENEFL:HUM_ALU_AT hypothetical LOC144404 hs
HC-G110:HUM_ALU_AT hypothetical LOC144404 hs
U133_X3P:HS.129926.0.A1_3P_AT hypothetical LOC144404 hs
HG-U95D:77671_AT hypothetical LOC144404 hs
HG-U133_PLUS_2:219154_AT Hypothetical LOC144404 hs
HG-U133A:219154_AT Hypothetical LOC144404 hs
HG-U133A_2:219154_AT Hypothetical LOC144404 hs
HU35KSUBA:AA282978_AT Hypothetical LOC144404 hs
HU35KSUBC:RC_AA018993_AT Hypothetical LOC144404 hs
U133_X3P:G13376014_3P_AT Hypothetical LOC144404 hs
HG-U95C:58489_AT Hypothetical LOC144404 hs
HG-U95E:82836_AT Hypothetical LOC144404 hs
HG-U95D:82892_AT Hypothetical LOC144404 hs
MG-U74BV2:113599_AT similar to transmembrane protein induced by tumor necrosis factor alpha mm
MOE430A:1427324_AT similar to transmembrane protein induced by tumor necrosis factor alpha mm
MOUSE430A_2:1427324_AT similar to transmembrane protein induced by tumor necrosis factor alpha mm
MOUSE430_2:1427324_AT similar to transmembrane protein induced by tumor necrosis factor alpha mm
MOE430B:1441510_AT Similar to transmembrane protein induced by tumor necrosis factor alpha, mRNA (cDNA clone IMAGE:4038363) mm
MOUSE430_2:1441510_AT Similar to transmembrane protein induced by tumor necrosis factor alpha, mRNA (cDNA clone IMAGE:4038363) mm
MU19KSUBB:TC30827_AT Similar to transmembrane protein induced by tumor necrosis factor alpha, mRNA (cDNA clone IMAGE:4038363) mm
MU19KSUBB:TC30828_AT Similar to transmembrane protein induced by tumor necrosis factor alpha, mRNA (cDNA clone IMAGE:4038363) mm
MU19KSUBB:TC30828_G_AT Similar to transmembrane protein induced by tumor necrosis factor alpha, mRNA (cDNA clone IMAGE:4038363) mm
Protein Similarities
Method ID Description E-Value
blast NP_497414 M01G5.3 [Caenorhabditis elegans] gb|AAC26943.2| Hypothetical protein M01G5.3 [Caenorhabditis elegans] 0.0
blast CAE66444 Hypothetical protein CBG11719 [Caenorhabditis briggsae] 1.0E-178
blast CAE66444 Hypothetical protein CBG11719 [Caenorhabditis briggsae] 1.0E-164
blast T33424 hypothetical protein M01G5.4 - Caenorhabditis elegans 2.0E-70
blast T33424 hypothetical protein M01G5.4 - Caenorhabditis elegans 1.0E-60
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497414.2 4 141-160,164-186,276-298,313-335
M01G5.3 4 141-160,164-186,276-298,313-335
GENEFINDER00000031213 3 182-200,315-337,352-374
Sequence
Target Sequence 
>C. ELEGANS:183578_AT
atggtggttgtcacatcactacctgtcgtgtgctgtgcctggaattgtgctcacctggaa
agatgggttgtgttatcaggaatttcgtccctacttcctcatcttcacattctacatctc
gctcgtccagttggctcaaaatcagtatcaatcaggctgcctccgccgcctacattccct
cggacagggacatcaaatggatattactgtagagggattcacgtcgtggcaattcaaagg
attaacatttttgttgccatttttggcgtttggatatgtttggactctttccctgttgct
cgggctcatcgccggcggaaatatcgtcaccacttcgatggtttgtattcggaagttcaa
aacgtccacttcgtacaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGTGGTTGTCACATCACTACCTG 646 49 13 Antisense
ACATCACTACCTGTCGTGTGCTGTG 293 105 25 Antisense
GTGTGCTGTGCCTGGAATTGTGCTC 511 487 40 Antisense
TTATCAGGAATTTCGTCCCTACTTC 478 679 85 Antisense
ATCAGTATCAATCAGGCTGCCTCCG 450 27 153 Antisense
GCCTACATTCCCTCGGACAGGGACA 39 281 180 Antisense
CTGTAGAGGGATTCACGTCGTGGCA 362 239 219 Antisense
TGGATATGTTTGGACTCTTTCCCTG 357 551 283 Antisense
ATCGCCGGCGGAAATATCGTCACCA 124 37 320 Antisense
ATCGTCACCACTTCGATGGTTTGTA 607 35 335 Antisense
GTTCAAAACGTCCACTTCGTACACA 289 439 367 Antisense
Sequence Source Affymetrix Proprietary Database