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[183563_at]

Description F31D5.2 /REP_DB=WormBase Gene ID /WP=CE19826 /TR=Q19932 /GB=AAC71105.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 69      4grp_highest_8_8 > 07      4grp_high_8_8 > 21      6grp_highest_nonorm > 92      6grp_total > 89      4grp_total > 18      6grp_highest_sub > In      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 149.9
(-2.1)
P 		135.7
(-3)
P 		143.9
(-2.5)
P 		235.8
(3)
P 		100
B 147
(-1.9)
P 		189.7
(-0.5)
P 		112.4
(-3)
P 		296.8
(3)
P 		184
C 129
(-3)
P 		183
(0.2)
P 		164.6
(-0.9)
P 		231.5
(3)
P 		103
avr 142
(-2.9) 		169.5
(-1.5) 		140.3
(-3) 		254.7
(3) 		114

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 283
1 2 3 4 5 6 range
A 191.9
(-2.1)
P 		306.4
(0.9)
P 		174.1
(-2.6)
P 		159.2
(-3)
P 		378.1
(2.8)
P 		384.6
(3)
P 		225
B 185.7
(-1.8)
P 		267.6
(0.2)
P 		139.3
(-3)
P 		203.6
(-1.4)
P 		378.8
(3)
P 		350.8
(2.3)
P 		240
C 150.2
(-2.9)
P 		302.5
(0.4)
P 		143.4
(-3)
P 		185.9
(-2.1)
P 		276.8
(-0.1)
P 		421.9
(3)
P 		279
avr 175.9
(-2.4) 		292.2
(0.6) 		152.3
(-3) 		182.9
(-2.2) 		344.6
(1.9) 		385.8
(3) 		234
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 54.6
(3)
P 		29.5
(-3)
P 		37.6
(-1.1)
P 		34.5
(-1.8)
P 		25
B 46.9
(0.4)
P 		46.9
(0.4)
P 		26.1
(-3)
P 		63.2
(3)
P 		37
C 42.7
(-1.8)
P 		52.6
(1.4)
P 		57.4
(3)
P 		39
(-3)
P 		18
avr 48.1
(3) 		43
(-1) 		40.4
(-3) 		45.6
(1.1) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 120
1 2 3 4 5 6 range
A 35.4
(-3)
P 		109
(2.6)
P 		37.9
(-2.8)
P 		40.4
(-2.6)
P 		114.1
(3)
P 		93.6
(1.4)
P 		79
B 64.6
(-1.5)
P 		89.1
(-0.2)
P 		33.8
(-3)
P 		53.9
(-2)
P 		153.9
(3)
P 		130.8
(1.8)
P 		120
C 55.7
(-1.9)
P 		92.3
(0.2)
P 		36.5
(-3)
P 		37.4
(-2.9)
P 		89.6
(0)
P 		141.2
(3)
P 		105
avr 51.9
(-1.9) 		96.8
(1.2) 		36.1
(-3) 		43.9
(-2.5) 		119.2
(2.8) 		121.9
(3) 		86
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183563_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5142
Sequence Type Exemplar sequence
Representative Public ID F31D5.2 NCBI
Target Description F31D5.2 /REP_DB=WormBase Gene ID /WP=CE19826 /TR=Q19932 /GB=AAC71105.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062340(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062340 NCBI Caenorhabditis elegans F31D5.2 (F31D5.2) mRNA, complete cds. 11/11 None
SNAP00000018593 ENSEMBL cdna:SNAP chromosome:CEL140:II:4215612:4218173:1 11/11 None
GENEFINDER00000018597 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4215612:4218173:1 11/11 None
F31D5.2 ENSEMBL cdna:known chromosome:CEL140:II:4215612:4218202:1 gene:F31D5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4215610-4218172(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F31D5.2
Entrez Gene 173754 Entrez gene
SwissProt Q19932 EMBL-EBI
WormBase CE29782 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71105 Hypothetical protein F31D5.2 [Caenorhabditis elegans] ref|NP_494741.2| F31D5.2 [Caenorhabditis elegans] 0.0
blast CAE74273 Hypothetical protein CBG21964 [Caenorhabditis briggsae] 0.0
blast CAE74273 Hypothetical protein CBG21964 [Caenorhabditis briggsae] 1.0E-180
blast AAP13750 Hypothetical protein Y52E8A.6 [Caenorhabditis elegans] ref|NP_001022461.1| Y52E8A.6 [Caenorhabditis elegans] 1.0E-107
Protein Domains
Database ID Description E-Value
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 8.0E-106
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494741.2 12 12-31,51-73,80-97,101-120,140-162,177-196,228-250,265-287,299-318,338-360,373-392,396-418
F31D5.2 12 12-31,51-73,80-97,101-120,140-162,177-196,228-250,265-287,299-318,338-360,373-392,396-418
SNAP00000018593 12 12-31,51-73,80-97,101-120,140-162,177-196,226-248,277-299,311-330,350-372,385-404,408-430
GENEFINDER00000018597 12 12-31,51-73,80-97,101-120,140-162,177-196,245-267,282-304,316-335,355-377,390-409,413-435
Sequence
Target Sequence 
>C. ELEGANS:183563_AT
tgtattgatttatctgttcttcccatatgatgcaccactacgtcctacacagaatgttgg
gatttttgagccaaatgcctacattgcaatattctgctctgggcttcttggtttcggcga
tgcaattattcagactcaagtctactcgttcctctgtgacgggtactctgaagaatcgtc
tcacgcatttgctctattcaagttctacagtgccatatcctccacaatagctttcttcgt
atcaaaatactttaccttggccggtcatcttctactttatggagcttttgccattctatc
agccatcgcagcagtcatggctcaaaagctatatttccacaagacacggcatttctttca
agaaaacacaaaaatccaccctgccatcgaatctgagccaataa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTATTGATTTATCTGTTCTTCCCA 92 561 987 Antisense
TTGCAATATTCTGCTCTGGGCTTCT 286 703 1070 Antisense
GGTACTCTGAAGAATCGTCTCACGC 235 505 1148 Antisense
TCAAGTTCTACAGTGCCATATCCTC 301 633 1184 Antisense
CTCCACAATAGCTTTCTTCGTATCA 500 235 1206 Antisense
GTATCAAAATACTTTACCTTGGCCG 324 453 1225 Antisense
CCGGTCATCTTCTACTTTATGGAGC 655 263 1247 Antisense
AGCTTTTGCCATTCTATCAGCCATC 344 79 1269 Antisense
GCCATCGCAGCAGTCATGGCTCAAA 280 279 1288 Antisense
ATATTTCCACAAGACACGGCATTTC 477 19 1317 Antisense
CCTGCCATCGAATCTGAGCCAATAA 650 265 1366 Antisense
Sequence Source Affymetrix Proprietary Database