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[183548_at]

Description C50F7.6 /REP_DB=WormBase Gene ID /WP=CE04244 /TR=Q18750 /GB=AAA83300.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 62      4grp_highest_8_8 > 08      4grp_high_8_8 > 44      6grp_highest_nonorm > 72      6grp_total > 62      4grp_total > 51      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 230
1 2 3 4 range
A 268.5
(0.6)
P 		330.7
(3)
P 		267.9
(0.6)
P 		175.8
(-3)
P 		155
B 303.9
(0.1)
P 		405.5
(3)
P 		313.2
(0.4)
P 		192.7
(-3)
P 		213
C 312.2
(0.7)
P 		333.5
(1.7)
P 		365.3
(3)
P 		223.8
(-3)
P 		142
avr 294.9
(0.7) 		356.6
(3) 		315.5
(1.5) 		197.4
(-3) 		159

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 271
1 2 3 4 5 6 range
A 295.1
(-3)
P 		358.5
(-0.8)
P 		464.9
(3)
P 		448.5
(2.4)
P 		349.6
(-1.1)
P 		328.7
(-1.8)
P 		170
B 270
(-3)
P 		327.6
(-1.4)
P 		482.8
(3)
P 		378.9
(0.1)
P 		386.3
(0.3)
P 		319.6
(-1.6)
P 		213
C 254.9
(-3)
P 		375
(-0.3)
P 		475.5
(1.9)
P 		525.8
(3)
P 		269.5
(-2.7)
P 		335.3
(-1.2)
P 		271
avr 273.3
(-3) 		353.7
(-0.6) 		474.4
(3) 		451.1
(2.3) 		335.1
(-1.2) 		327.9
(-1.4) 		201
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 97.8
(3)
P 		71.8
(0.8)
P 		70
(0.7)
P 		25.7
(-3)
P 		72
B 96.9
(2.7)
P 		100.2
(3)
P 		72.6
(0.2)
P 		41.1
(-3)
P 		59
C 103.4
(1.4)
P 		95.9
(0.9)
P 		127.3
(3)
P 		37.7
(-3)
P 		90
avr 99.4
(3) 		89.3
(2.1) 		90
(2.1) 		34.8
(-3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 54.4
(-3)
P 		127.5
(3)
P 		101.2
(0.8)
P 		113.7
(1.9)
P 		105.5
(1.2)
P 		80
(-0.9)
P 		73
B 94
(-3)
P 		109
(-1.6)
P 		117.2
(-0.8)
P 		100.4
(-2.4)
P 		156.9
(3)
P 		119.2
(-0.6)
P 		63
C 94.5
(-1.7)
P 		114.4
(1.8)
P 		121
(3)
P 		105.7
(0.3)
P 		87.2
(-3)
P 		112.2
(1.4)
P 		34
avr 81
(-3) 		117
(3) 		113.1
(2.4) 		106.6
(1.3) 		116.5
(2.9) 		103.8
(0.8) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183548_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10077
Sequence Type Exemplar sequence
Representative Public ID C50F7.6 NCBI
Target Description C50F7.6 /REP_DB=WormBase Gene ID /WP=CE04244 /TR=Q18750 /GB=AAA83300.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068861(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068861 NCBI Caenorhabditis elegans C50F7.6 (C50F7.6) mRNA, complete cds. 11/11 None
SNAP00000024185 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7720617:7723181:-1 11/11 None
GENEFINDER00000024198 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7720617:7723181:-1 11/11 None
C50F7.6 ENSEMBL cdna:known chromosome:CEL140:IV:7720559:7723183:-1 gene:C50F7.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7720622-7723187(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C50F7.6
Entrez Gene 177553 Entrez gene
SwissProt Q18750 EMBL-EBI
WormBase CE04244 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.15408.1.S1_AT similar to PDZ domain containing RING finger protein 4 (Ligand of Numb-protein X 4) (SEMACAP3-like protein) cfa
CANINE_2:CFAAFFX.15413.1.S1_S_AT similar to PDZ domain containing RING finger protein 4 (Ligand of Numb-protein X 4) (SEMACAP3-like protein) cfa
CANINE_2:CFAAFFX.15417.1.S1_AT similar to PDZ domain containing RING finger protein 4 (Ligand of Numb-protein X 4) (SEMACAP3-like protein) cfa
CHICKEN:GGA.19164.1.S1_AT PDZ domain containing RING finger 4 gga
HG-U133_PLUS_2:220595_AT PDZ domain containing RING finger 4 hs
HG-U133A:220595_AT PDZ domain containing RING finger 4 hs
HG-U133A_2:220595_AT PDZ domain containing RING finger 4 hs
HU35KSUBB:RC_R37079_AT PDZ domain containing RING finger 4 hs
U133_X3P:G7242145_3P_AT PDZ domain containing RING finger 4 hs
HG-U95C:64526_AT PDZ domain containing RING finger 4 hs
MOUSE430_2:1456512_AT PDZ domain containing RING finger 4 mm
MOE430B:1456512_AT PDZ domain containing RING finger 4 mm
MG-U74CV2:168462_AT PDZ domain containing RING finger 4 mm
RAE230B:1393685_AT PDZ domain containing RING finger 4 (predicted) rn
RAT230_2:1393685_AT PDZ domain containing RING finger 4 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAA83300 Hypothetical protein C50F7.6 [Caenorhabditis elegans] ref|NP_501262.1| C50F7.6 [Caenorhabditis elegans] 0.0
blast CAE60716 Hypothetical protein CBG04388 [Caenorhabditis briggsae] 0.0
blast AAH77302 Unknown (protein for MGC:80196) [Xenopus laevis] 2.0E-19
Sequence
Target Sequence 
>C. ELEGANS:183548_AT
gattccgaattccatcgtattcagttgtattgaaagaagaaaaacgaaaaagttctttcc
gaattccagttccgcattactttcgatcctcaaaagcgaaatcaaaagtatatcaagttg
atgctccagatgaagaaattgaagaagttattgatgaagagaaaatggctgcaaagactg
atttgggagacacaactgttcattacaaatggaaagttaaacgaagatgtgatggatcaa
gatatattgtcaaaagaccaattcgaagtcaaattttgaaaaaaagagaagcacaggtgt
atcgtgaacgagcaccaatttcaaccgatgatgatgcaatgagcgaattaaagcttggaa
ggtatcacacgaaggaagaaagaaaaaggatattggaaagagagaacacgaaaaaaatgt
tgaagcttcagcaaaaaatgatggaaaaggcacatccgtcggagcaggtaatatatcaaa
tgtcacaacaaaagcttgctcgccaaaaagacgcaatggtaatggatgaatttgtaacaa
caagagaagtactttcaagtcgaacacgcccagatggtatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCCGAATTCCATCGTATTCAGT 444 433 659 Antisense
CGAATTCCAGTTCCGCATTACTTTC 142 255 718 Antisense
GCATTACTTTCGATCCTCAAAAGCG 409 305 732 Antisense
GATTTGGGAGACACAACTGTTCATT 295 427 838 Antisense
AGCACAGGTGTATCGTGAACGAGCA 271 77 948 Antisense
AACGAGCACCAATTTCAACCGATGA 39 145 965 Antisense
AAGCTTGGAAGGTATCACACGAAGG 175 151 1009 Antisense
TGGAAAAGGCACATCCGTCGGAGCA 654 545 1100 Antisense
GTCACAACAAAAGCTTGCTCGCCAA 457 463 1140 Antisense
AGTACTTTCAAGTCGAACACGCCCA 681 61 1206 Antisense
AAGTCGAACACGCCCAGATGGTATT 661 157 1215 Antisense
Sequence Source Affymetrix Proprietary Database